Effect of endogenous purines on electrically evoked <scp>ACh</scp> release at the mouse neuromuscular junction

Sanabria, Javier González; Paso, Maximiliano Hurtado; Frontera, Tamara; Losavio, Adriana S.

doi:10.1002/jnr.25107

Cited by 3 publications

(1 citation statement)

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“…Much less is known about action of purines on the muscles of warm-blooded animals. Experiments on synapses in the rat diaphragm showed a presynaptic potentiating effect of activation of P2 receptors leading to the facilitation of the release of acetylcholine [11], and later the inhibitory effect of adenosine and ATP on end plate potentials was demonstrated at the same object [12]. It is clear that further evaluation of the effects of these purines on other skeletal muscles of warm-blooded animals is needed.…”

Section: Introductionmentioning

confidence: 99%

Presynaptic Purinergic Modulation of the Rat Neuro-Muscular Transmission

Khairullin,

Grishin,

Ziganshin

2023

CIMB

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ATP, being a well-known universal high-energy compound, plays an important role as a signaling molecule and together with its metabolite adenosine they both attenuate the release of acetylcholine in the neuro-muscular synapse acting through membrane P2 and P1 receptors, respectively. In this work, using a mechanomyographic method, we analyzed the presynaptic mechanisms by which ATP and adenosine can modulate the transduction in the rat m. soleus and m. extensor digitorum longus. N-ethylmaleimide, a G-protein antagonist, prevents the modulating effects of both ATP and adenosine. The action of ATP is abolished by chelerythrin, a specific phospholipase C inhibitor, while the inhibitory effect of adenosine is slightly increased by Rp-cAMPS, an inhibitor of protein kinase A, and by nitrendipine, a blocker of L-type Ca2+ channels. The addition of DPCPX, an A1 receptor antagonist, fully prevents the inhibitory action of adenosine in both muscles. Our data indicate that the inhibitory action of ATP involves metabotropic P2Y receptors and is mediated by phospholipase C dependent processes in rat motor neuron terminals. We suggest that the presynaptic effect of adenosine consists of negative and positive actions. The negative action occurs by stimulation of adenosine A1 receptors while the positive action is associated with the stimulation of adenosine A2A receptors, activation of protein kinase A and opening of L-type calcium channels. The combined mechanism of the modulating action of ATP and adenosine provides fine tuning of the synapse to fast changing conditions in the skeletal muscles.

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Section: Introductionmentioning

confidence: 99%