Effect of enclosing terpenoids-rich Boswellia Carterri ethyl acetate extract in binary cyclodextrin based oligomer nano-complex for improving its activity via counteracting ICAM-1, Ilβ4 and LTB4 pathways in respiratory distressed rats

Abstract

Boswellia carterii (BC) resins plants have a long historical background as a treatment for inflammation, as indicated by information originating from multiple countries. Twenty-seven diterpenoids have been identified in ethylacetate and total methanol BC, comprising seventeen boscartins of the cembrane-type diterpenoids and ten boscartols of the prenylaromadendrane-type diterpenoids. Moreover, twenty-one known triterpenoids have also been found, encompassing nine tirucallane-type, six ursane-type, four oleanane-type, and two lupane-type. The cembrane-type diterpenoids hold a significant position in pharmaceutical chemistry and related industries due to their captivating biological characteristics and promising pharmacological potentials. Extraction of BC, creation and assessment of nano sponges loaded with either B. Carterii plant extract or DEX, are the subjects of our current investigation. With the use of ultrasound-assisted synthesis, nano sponges were produced. The entrapment efficiency (EE%) of medications in Nano sponges was examined using spectrophotometry. Nano sponges were characterized using a number of methods. Within Nano sponges, the EE% of medicines varied between 98.52± 0.07 and 99.64± 1.40%. The nano sponges' particle sizes varied from 105.9±15.9 to 166.8±26.3 nm. Drugs released from Nano sponges using the Korsmeyer-Peppas concept. In respiratory distressed rats, the effects of BC plant extract, DEX salt and their nano formulations (D1, D5, P1 and P1), were tested. Treatment significantly reduced ICAM-1, LTB4, and ILβ4 levels and improved histopathologic profiles, when compared to the positive control group. Boswellia extract and its Nano sponge formulation P1 showed promising therapeutic effects. The effect of P1 may be due to synergism between both the extract and the formulation. This effect was achieved by blocking both ICAM-1 and LTB4 pathways, therefore counteracting the effects of talc powder.