Effect of EMS on Induced Morphological Mutants and Chromosomal Variation in Cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp)

Gnanamurthy, S.; Dhanavel, D.

doi:10.56431/p-i0xny2

Cited by 7 publications

(7 citation statements)

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“…The disparities, such as plant height and habit, leaf morphology, and pod variation, were considered monogenic recessive. Tall and dwarf mutants were observed, which were also earlier observed by Gnanamurthy and Dhanavel (2014) in cowpea and Shahwar et al. (2019) in lentil.…”

Section: Discussionsupporting

confidence: 76%

“…Variations in the seed attributes of mutagenized fenugreek were recorded. Several workers have reported variations in crops such as cowpea ( Gnanamurthy and Dhanavel, 2014 ), black gram ( Goyal et al., 2020 ), and linseed ( Jahan et al., 2020 ). These variations in seeds may be attributed to the mutagen-induced disruption of one or few genes linked with these characteristics.…”

Section: Discussionmentioning

confidence: 99%

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Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Naaz

Choudhary²,

Sharma³

et al. 2023

Front. Plant Sci.

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Trigonella foenum graecum L. (Fenugreek) is a valuable medicinal plant cultivated for decades for its therapeutic characteristics. Still no pronounced improvement concerning wild form was accomplished as it is a self-pollinating crop. Induced mutagenesis is encouraged as a remarkable tool on this plant to circumvent the genetic bottleneck of cultivated germplasms. As a result, novel allelomorphic combinations for short-term agronomic attributes were developed. Fenugreek cultivar Pusa Early Bunching, selected for the present experiment, was mutagenized with five doses (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) of caffeine and sodium azide (SA) to evaluate its impact on the qualitative and quantitative traits of M1 and M2 generation conducted in a Complete Randomized Block Design (CRBD), replicated five times during 2019–2020 and 2020–2021, respectively. The frequency of induced phenotypic variations was assessed in M2 progenies, resulting in the identification and isolation of a broad spectrum of mutants with altered phenotypes. Mutagenic effectiveness and efficiency were found to be maximum at lower concentrations of the mutagen treatments and highest in SA, followed by caffeine. Various morphological mutants with modified characters were observed at different concentrations in M2 generation. The spectrum of mutations was wider in SA than in caffeine, as caffeine produced 51 while SA produced 54 individual mutants under seven major categories. The maximum frequency of morphological mutants was associated with leaf, followed by plant size, plant growth habit, pod, seed size, seed shape, and seed color. Morphological and structural variations in the guard cells of stomata and seeds were observed through scanning electron microscopy. The variations created in the economically important traits may enrich the genetic diversity of this plant species. Moreover, these morphological mutants may serve as a source of elite genes in further breeding programs of fenugreek.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Naaz

Choudhary²,

Sharma³

et al. 2023

Front. Plant Sci.

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“…Similar results were recorded by Maryam (2017); and Maryam and Hussaini (2023). Numerous crops, including cluster beans (Velu et al, 2007), cowpea (Gnanamurthy et al 2013)…”

Section: Journal Of Basics and Applied Sciences Researchmentioning

confidence: 99%

Stimulating Genetic Variability on Growth And Yield of Okra (Abelmoschus esculentus) Using Sodium Azide

Ati,

Chiroma,

Ahmed

2024

jobasr

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As interest in mutant breeding grows, so does the amount of study being done on employing mutagens to induce mutations for genetic variety. As a result, breeders' only means of producing variety is through mutation breeding. An experiment was conducted to induce genetic variability using sodium azide on varieties of okra (Ablemoschus esculentus). Okra seeds were treated with different sodium azide concentrations (0mM, 2mM, 4mM, and 6mM). The research was carried out at the Department of Agronomy Federal University Dutsin-ma. The objectives of the research were to evaluate the effect of sodium azide (NaN3) on the growth and yield of okra, identify the mutagen that induces beneficial mutation with minimum lethality, select the variety of okra that produce the best mutants in terms of yield and agronomic traits. The pre-soaking of okra seed in distilled water, treatment with mutagen, seed germination test, and germination percentage were done in the laboratory. The treated and the control seeds were planted in nylon bags on the field. Each treatment was in three replications. The field layout was Completely Randomized design (CRD). The result revealed that plots treated with 2mM, 4mM, and 6mM of sodium azide recorded decreased growth, yield, and yield components with an increase in concentrations of the mutagens in okra varieties studied in comparison with untreated plots. However, treatment with 2mM and 4mM performed better than those treated with 6mM. Therefore, treatment with a low concentration of Sodium Azide is recommended for the okra varieties used in this research

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“…By causing guanine to get alkylated at either its N7 or O6 position, EMS replaces the base pair of cytosine with a pair of thymine [5]. High gene mutation frequencies and low chromosome aberration frequencies are reported by EMS [6].…”

Section: Induction Of Mutation In the Bacillus Subtilis Strain (Mtcc ...mentioning

confidence: 99%

Induction of mutation in the Bacillus subtilis strain (MTCC 6808) through chemical mutagens

Singh

Chaudhary

Misra

et al. 2023

ijhs

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Through the creation of greater genetic variety in already existing varieties, induced mutagenesis has been an essential component in the process of improving variations and facilitating the development of superior types. The generation of genetic variants can be accomplished in a strong manner by causing mutations in bacteria via chemical and physical mutagenesis. Chemical mutagens are typical tools to originate mutations in a number of plants and microbes. Mutagenesis is the process of altering the genetic makeup of an organism by introducing a new mutation. Consequently, we have utilized chemical mutagen in order to induce mutation in the Bacillus subtilis (MTCC 6808) strain. During this investigation, a mutagen solution is prepared from EMS in varying concentrations. After incubation, we compared the samples with controls to determine whether the mutation had taken place. Therefore, it is possible, all things considered, to induce a certain type of mutation by making use of the proper mutagen and bacterial strain.

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Effect of EMS on Induced Morphological Mutants and Chromosomal Variation in Cowpea (<i>Vigna unguiculata</i> (L.) Walp)

Cited by 7 publications

References 0 publications

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Stimulating Genetic Variability on Growth And Yield of Okra (Abelmoschus esculentus) Using Sodium Azide

Induction of mutation in the Bacillus subtilis strain (MTCC 6808) through chemical mutagens

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