Effect of emptying the vasculature before performing regional limb perfusion with amikacin in horses

Abstract

Emptying the vasculature with an Esmarch bandage before IV-RLP can improve amikacin concentrations in the metacarpophalangeal joint of horses with no effect on the levels of amikacin in the radiocarpal joint.

Evaluation of 10‐minute versus 30‐minute tourniquet time for intravenous regional limb perfusion with amikacin sulfate in standing sedated horses

“…AS concentrations (μg/ml) were determined by use of a fluorescence polarisation immunoassay (Roche Kinetic Interaction of Microparticles in Solution –KIMS screening assay. Cobas c311 analyzer, Roche Diagnostics, Mannheim, Germany) (kinetic interaction of microparticles in solution as measured by changes in light transmission) as previously reported (Parra-Sanchez and others 2006, Mahne and others 2014, Sole and others 2015). Testing was performed at the Biochemical Laboratory of the William R Pritchard Veterinary Medical Teaching Hospital.…”

Regional limb perfusion in horses

“…Furthermore, using a vein proximal to the carpus or tarsus is useful when the distal limb suffers from trauma since it allows for immobilisation of the region with a cast when indicated (Kelmer and others 2012). Recently, most RLP studies have used the cephalic vein and it appears that using the proximal vein is the preferred site for catheter placement (Beccar-Varela and others 2011, Zantingh and others 2014, Aristizabal and others 2016, Kilcoyne and others 2016, Oreff and others 2016, Sole and others 2016). …”

“…The reasoning behind this is to decrease antimicrobial dilution in blood, improve penetration of the tissue since the blood vessels are empty and avoid leakage under the tourniquet by decreasing the intravascular pressure (Sole and others 2016). …”

Pharmacokinetics of ceftazidime after regional limb perfusion in standing horses

“…The constant battle against resistant bacteria creates a growing need for the development of more efficient treatments. As a result, significant research effort has recently focused on RLP, such as investigating combinations of antimicrobials, the use of additional types of antimicrobials, and improving the technique …”