Effect of emodin on pancreatic fibrosis in rats

Abstract

AIM:To establish the rats model of chronic fibrosing pancreatitis and to prove the anti-fibrotic effect of emodin in chronic pancreatitis with fibrosis. METHODS:Fifty rats were randomly divided into five groups, 10 rats in each group. Trinitrobenzene sulfonic acid (TNBS) was infused into the pancreatic duct to induce chronic pancreatitis in rats (except for normal group). Emodin-treated rats were fed with different doses of emodin (20, 40 and 80 mg/kg body weight) for 28 d, while normal group and control group received 0.9% sodium chloride solution. Serum levels of hyaluronic acid (HA) and laminin (LN) were determined by radioimmunoassay. Histopathological alterations were studied by optical microscopy. Expression of collagen was also examined while transforming growth factorbeta-1 (TGF-β1) was localized by immunochemistry. RESULTS:In emodin-treated rats, the serum levels of HA and LN were decreased significantly (HA, 62.2 ± 19.3 µg/L vs 112.7 ± 26.5 µg/L, P < 0.05; LN 44.3 ± 10.4 µg/L vs 86.2 ± 16.5 µg/L, P < 0.05); the degree of fibrosis was ameliorated observably; the expression of collagen in pancreatic tissue was reduced especially in high-dose emodin-treated group (36% ± 5% vs 42% ± 6%, P < 0.05); with the increased doses of emodin, the expression of TGF-β1 was declined, compared with those in control group. CONCLUSION:Emodin has an anti-fibrotic effect on pancreatic fibrosis in rats. Because of its anti-fibrotic effect, it could be a potential herb for the treatment of chronic pancreatitis.

Emodin Regulates Glucose Utilization by Activating AMP-activated Protein Kinase*

“…As a result of this screening, emodin (1,3,8-trihydroxy-6-methylanthraquinone), a natural active compound in the herb Rheum palmatum L. was shown to stimulate the AMPK pathway. Some previous studies have suggested that emodin has an beneficial effect on energy metabolism, including adipocyte differentiation (14), anti-fibrotic effect on pancreatic fibrosis (15), and liver (16). However, validation of the molecular mechanism and the anti-diabetic effects of this compound has not been fully examined.…”

Evaluation of Phytoconstituents of Bryonopsis laciniosa fruit by UV-Visible Spectroscopy and FTIR analysis

“…26 Emodins has anti angiogenetic effect 27 Plenty of pharmaceutical studies indicates that emodins have anti inflammatory, anticancer and antimicrobial activity anti-cancer, anti-microbial and antiinflammatory effects. 28 The UV-Visible spectra were performed to identify the compounds containing σ-bonds, π-bonds, and lone pair of electrons, chromophores and aromatic rings The profile showed the peaks at 208.04, 271.94 and 881.96 nm with the absorption 2.76,0.72 and 0.04 respectively Table 3 and Figure 1. The result confirms the occurrence of peaks at 208-881 nm reveals that the absorption bands are due to the presence of flavonoids, phenol and its derivatives in the bryonopsis laciniosa fruit.…”

Emodin inhibits extracellular matrix synthesis by suppressing p38 and ERK1/2 pathways in TGF-β1-stimulated NRK-49F cells

“…It has been demonstrated to inhibit the fibrosis process of various organs, including the liver, pancreas, peritoneum and kidney (1)(2)(3). Emodin suppresses p38 phosphorylation to inhibit cell proliferation and fibronectin expression in mesangial cells cultured in high-glucose solution (4).…”