Effect of Emdogain^® on human periodontal fibroblasts in an in vitro wound‐healing model

Abstract

Therefore, EMD appears to exert an influence on cells that is compatible with improved wound healing.

Emdogain® in carcinogenesis: a systematic review of in vitro studies

“…It has been shown that EMD has no effect on ALP activity (59); however, EMD both decreases (57) and increases (62,73,74) ALP activity in PDL fibroblasts. The production of osteopontin and osteoprotegerin has been shown to be increased by EMD (60,74). In addition, although EMD enhances bone-like nodule formation, it does not induce osteoblastic differentiation of PDL or gingival fibroblasts (23,55,59,62).…”

The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization

“…Recent clinical studies have demonstrated that the EMP matrix supports recolonization with mesenchymal cells [4]. In vitro, periodontal ligament cells derived from the mesenchyme, when cultured on EMP, increased their attachment rate, growth rate and metabolism, and subsequently released several growth factors and extracellular matrix (ECM) molecules including fibronectin at elevated levels into the medium [5][6][7][8][9][10][11]. Periodontal ligament cells have the ability to migrate, proliferate and differentiate into osteoblast-like and cementoblast-like cells, thereby promoting tissue regeneration.…”

Effect of Smoking on Attachment of Human Periodontal Ligament Cells to Periodontally Involved Root Surfaces Following Enamel Matrix Derivative Application

“…Gamal et al (12) observed significantly higher cell adherence in the healthy control group than in the periodontally-diseased treated control, suggesting that cultured human PDL fibroblasts prefer not to adhere to periodontally diseased treated root surfaces. Despite a number of reports on the role of EMD in periodontal regeneration (14,23,24), especially on PDL cells (8,14,20,28,38) and wound healing (31), different phenotypes of PDL fibroblasts can be isolated and may respond differently to regeneration attempts through biomimetics, which may explain the seemingly contradictory results in published studies regarding the in vitro effects of EMD (7). Chong et al (9) showed that EMD alone has a limited effect on PDL fibroblast proliferation.…”

“…Many in vitro studies have focused on the mechanism of action of EMD in periodontal ligament (PDL) cells during periodontal regeneration. In vitro studies demonstrate that EMD treatment of PDL fibroblasts stimulates proliferation, protein and collagen synthesis, and also induces mineralization (8,14,20,23,24,28,31,33,38).…”