Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers

Erdem, Hüseyin; Karaşahi̇n, Tahir; Alkan, Hasan; Dursun, Şükrü; Satılmış, Fatma; Güler, Mehmet

doi:10.1007/s11250-020-02287-6

Cited by 22 publications

(21 citation statements)

References 31 publications

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“…The newer studies confirm this correlation in both dairy and meat cattle (Tab. 1) [12][13][14]. The probability of conception after the transfer of poor quality embryos was even 31% lower than in the case of 1st class embryos [9].…”

Section: Factors Associated With the Embryo Sourcingmentioning

confidence: 89%

“…The percentage of pregnant females after application of frozen embryos in the morula stage was 54.3% and in the early blastocyst stage 59.7% [19]. In the newest studies by Ferraz et al [6] and Erdem et al [14] a significantly higher probability of pregnancies after embryo transfer in the expanding blastocyst stage than in the blastocyst, early blastocyst and morula stage were recorded (Tab. 1).…”

Section: Factors Associated With the Embryo Sourcingmentioning

confidence: 91%

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Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Wieczorkiewicz

Jaśkowski

Wichtowska

et al. 2021

Medical Journal of Cell Biology

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Multiple Ovulation Embryo Transfer is a biotech method with more than 50 years of history and an established position in cattle breeding. This procedure is beneficial in many ways, but it also carries a risk of failure. The study presents the overview of the most important risk factors that may affect conception rates in the course of embryo transfer, including the factors associated with the embryo sourcing (embryo production method, embryo quality, development stage and breed, embryo storage method), embryo transfer procedure (synchrony/asynchrony, embryo transfer difficulty, the time of the transcervical insemination gun passage, depth of embryo deposition, localization and structure of the corpus luteum relative to the follicle and both individual characteristics of donors and recipients (level of concentration of progesterone, the state of health of the udder, lactation level, body condition score and age) and some environmental factors.

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Section: Factors Associated With the Embryo Sourcingmentioning

confidence: 89%

Section: Factors Associated With the Embryo Sourcingmentioning

confidence: 91%

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Wieczorkiewicz

Jaśkowski

Wichtowska

et al. 2021

Medical Journal of Cell Biology

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“…Recipient candidates on the 7th day of the oestrous cycle were examined by rectal palpation and real‐time ultrasonography. Accordingly, animals with ≥15 mm corpus luteum in the ovary were chosen to be recipients (Alkan et al., 2020; Erdem et al., 2020). ET was performed to the anterior 1/3 of the uterine horn at the side of the corpus luteum after administering upper epidural anaesthesia.…”

Section: Methodsmentioning

confidence: 99%

“…In studies, it was stated that pregnancy rates ranged between 30% and 60% after transferable embryos were transferred to suitable recipients in cattle (Map letoft & Bo, 2016; Vasconcelos et al., 2011). The factors affecting pregnancy rates after ET include the transfer side, transfer location, cervix transfer score, type and diameter of the corpus luteum, embryonic stage and quality, season, age, fresh or frozen transfer, nutrition, stress and the difference in oestrous synchrony between donor and recipients (Alkan et al., 2020; Erdem et al., 2020; Ferraz et al., 2016; Julon et al., 2018; Rodrigues et al., 2018; Roper et al., 2018; Vieira et al., 2014). Moreover, cattle breeds selected as recipients in ET are known to have an effect on the pregnancy rate (Arreseigor et al., 2016; Hasler, 2004; Looney et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of flunixin meglumine treatment during and after embryo transfer on the pregnancy rate in cattle

Karaşahi̇n

Alkan

Satılmış

et al. 2021

Reprod Domestic Animals

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Contents This study aimed to determine the effect of flunixin meglumine treatment during and after the transfer of in vivo produced embryos to Angus (cows) and Holstein (cows and heifers) breeds of cattle on pregnancy rate. Holstein cows were used as donors in the study. A double dose of prostaglandin F2α was administered to the recipient animals for synchronization. Uterine flushing was performed in donors on day 7 after artificial insemination. A total of 295 transferable embryos were obtained. These embryos were transferred to Angus cows (n = 85), Holstein heifers (n = 80) and Holstein cows (n = 130). After the transfer, these animals were divided into three subgroups. The first subgroup (TI) was administered flunixin meglumine during embryo transfer, and the second subgroup (TII) was administered flunixin meglumine both during embryo transfer and on days 8 and 9 after the transfer. The third subgroup (TIII) was not administered anything and it was considered the control group. Pregnancy examination of the recipients was performed on days 30–35 after the transfer using real‐time ultrasonography. The pregnancy rates after embryo transfer were found to be 43.52% in Angus cows, 42.5% in Holstein heifers, and 24.61% in Holstein cows (p < .05). When the animals were not classified according to breed, the pregnancy rates in subgroups TI, TII and TIII were found to be 29.29%, 45.10% and 29.79%, respectively (p < .05). In addition, the pregnancy rates were higher in TII and TIII subgroups of Angus cows and Holstein heifers compared to that of Holstein cows (p < .05). As a result, the pregnancy rates obtained after embryo transfer in Angus cows and Holstein heifers were found to be higher than that in Holstein cows. In addition, it was concluded that the administration of flunixin meglumine during and during/after embryo transfer has a positive effect on pregnancy rates in Angus cows and Holstein heifers.

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“…A study by Madureira et al (2019) reported that cows with greater intensity of estrous expression have greater concentration of P4 post-AI and greater pregnancy per AI. Greater pregnancy per ET are associated when embryo transfer occurs with excellent and good quality embryos (Ferraz et al, 2016) and embryos in later stages of embryonic development (Erdem et al, 2020). However, the association of stage of embryo development and the quality of the transferred embryo with the occurrence and intensity of estrous behavior has yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and greater intensity of estrus in recipient lactating dairy cows improve pregnancy per embryo transfer

Madureira

Burnett

Marques

et al. 2022

Journal of Dairy Science

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The aim of this study was to determine the association between occurrence and intensity of estrous expression with pregnancy success in recipient lactating dairy cows subjected to embryo transfer (ET). Two observational studies were conducted. Holstein cows were synchronized using the same timed ET protocol, based on estradiol and progesterone in both experiments. At 9 d after the end of the timed ET protocol only animals that had ovulated were implanted with a 7-d embryo [experiment 1 (Exp. 1); n = 1,401 ET events from 1,045 cows, and experiment 2 (Exp. 2); n = 1,147 ET events from 657 cows]. Embryos were produced in vivo (Exp. 1 and Exp. 2) and in vitro (only Exp. 2), then transferred to recipient cows as fresh or frozen-thawed. Pregnancy was confirmed at 29 and 58 d after the end of timed ET protocol. In Exp. 1, animals had their estrous expression monitored through a tail chalk applied on the tail head of the cows and evaluated daily for chalk removal (no estrus: 100% of chalk remaining; estrus: <50% of chalk remaining). In Exp. 2, cows were continuously monitored by a leg-mounted automated activity monitor. Estrous expression was quantified using the relative increase in physical activity at estrus in relation to the days before estrus. Estrous expression was classified as no estrus [<100% relative increase in activity (RI)], weak intensity (100-299% RI), and strong intensity (≥300% RI). Data were analyzed by analysis of variance using mixed linear regression models (GLIMMIX) in SAS (SAS Institute Inc.). A total of 65.2% (914/1,401) and 89.2% (1,019/1,142) of cows from Exp. 1 and Exp.2, respectively, displayed estrus at the end of the ovulation synchronization protocol. In Exp. 1, cows expressing estrus before to ET had greater pregnancy per ET than those that did not [41.0 ± 2.3% (381/914) vs. 31.5 ± 2.9% (151/487), respectively]. Similarly, in Exp. 2, cows classified in the strong intensity group had greater pregnancy per ET compared with cows in the weak intensity and no estrus groups [41.3 ± 2.2% (213/571) vs. 32.7 ± 2.7% (115/353) vs. 11.3 ± 3.5% (26/218), respectively]. There was no effect of ET type on pregnancy per ET in Exp. 1. However, in Exp. 2, cows that received an in vivo-produced embryo, either fresh or frozen, had greater pregnancy per ET compared with cows that received in vitro-produced embryo. Cows receiving embryos in the early blastocyst and blastocyst stage had greater fertility compared with cows receiving embryos in the morula stage. There was an interaction between the occurrence of estrus and the stage of embryo development on pregnancy per ET, cows which displayed estrus and received a morula or early blastocyst had greater pregnancy per ET than cows that did not display estrus. In conclusion, the occurrence and the intensity of estrous expression improved pregnancy per ET in recipient lactating dairy cows and thus could be used as a tool to assist in the decision making of reproduction strategies in dairy farms.

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Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers

Cited by 22 publications

References 31 publications

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Effect of flunixin meglumine treatment during and after embryo transfer on the pregnancy rate in cattle

Occurrence and greater intensity of estrus in recipient lactating dairy cows improve pregnancy per embryo transfer

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