Effect of ELOVL6 on the lipid metabolism of bovine adipocytes

Junjvlieke, Zainaguli; Khan, Rajwali; Mei, Chugang; Chen, Gong; Wang, Sihu; Raza, Sayed Haidar Abbas; Hong, Jie; Wang, Xiaoyu; Yang, Wenguang; Zan, Linsen

doi:10.1016/j.ygeno.2019.12.024

Cited by 31 publications

(21 citation statements)

References 37 publications

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“…After 48 h of transfection, we used the RNAiso Plus Kit (Trizol, Takara, Beijing, China) to extract RNA. The specific extraction method can refer to the reference (Junjvlieke et al, 2020). After we removed the culture medium from the cells, we washed the cells with PBS 2∼3 times in each well, then added 1 mL Trizol, placed the mixture at 4 • for 4 min, and transferred the mixture to a 1.5 mL centrifuge tube.…”

Section: Differential Expression and Pathway Enrichment Analysesmentioning

confidence: 99%

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Chen

Raza

et al. 2021

Front. Genet.

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Micro RNA (miR) are recognized for their important roles in biological processes, particularly in regulatory componentization. Among the miR, miR-150 has been the focus of intense scrutiny, mostly due to its role in malignant tumors. A comparison between steer and bull adipose tissues identified bta-miR-150 as one of the nine downregulated miRNAs, although its function remains unknown (GEO:GSE75063). The present study aimed to further characterize the role of bta-miR-150 in cattle. bta-miR-150 has a negative regulatory effect on the differentiation of bovine adipocytes and promotes proliferation. Overexpression of bta-miR-150 can promote mRNA and protein expression of the marker genes CDK1, CDK2, and PCNA, increase the number of EdU-stained cells, promote adipocyte proliferation, inhibit adipocyte differentiation, and reduce lipid droplet formation. Results of RNA-seq and WGCNA analyses showed that the mammalian target of the rapamycin signaling pathway, which plays a major regulatory role, is dysregulated by the overexpression and inhibition of miR-150. We found that the target gene of bta-miR-150 is AKT1 and that bta-miR-150 affects AKT1 phosphorylation levels. These results showed that bta-miR-150 plays a role in adipogenic differentiation and might therefore have applications in the beef industry.

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Section: Differential Expression and Pathway Enrichment Analysesmentioning

confidence: 99%

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Chen

Raza

et al. 2021

Front. Genet.

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“…All these pathways have proven roles in adipogenesis. Previously, it has been reported that PI3K-Akt signaling pathway plays an important role in adipose tissue function and energy metabolism through regulation of the genes expressed in lipid metabolism [ 74 , 75 , 76 ]. Previous literature has shown the association of FoxO signaling, PI3K-Akt signaling, and cAMP signaling pathways with lipid metabolism [ 77 , 78 , 79 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Reveals Function of Bta-miR-149-5p in the Regulation of Bovine Adipocyte Differentiation

Guo

Khan

Raza

et al. 2021

Animals

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Intramuscular fat is a real challenge for the experts of animal science to improve meat quality traits. Research on the mechanism of adipogenesis provides invaluable information for the improvement of meat quality traits. This study investigated the effect of bta-miR-149-5p and its underlying mechanism on lipid metabolism in bovine adipocytes. Bovine adipocytes were differentiated and transfected with bta-miR-149-5p mimics or its negative control (NC). A total of 115 DEGs including 72 upregulated and 43 downregulated genes were identified in bovine adipocytes. The unigenes and GO term biological processes were the most annotated unigene contributor parts at 80.08%, followed by cellular component at 13.4% and molecular function at 6.7%. The KEGG pathways regulated by the DEGs were PI3K-Akt signaling pathway, calcium signaling pathway, pathways in cancer, MAPK signaling pathway, lipid metabolism/metabolic pathway, PPAR signaling pathway, AMPK signaling pathway, TGF-beta signaling pathway, cAMP signaling pathway, cholesterol metabolism, Wnt signaling pathway, and FoxO signaling pathway. In addition to this, the most important reactome enrichment pathways were R−BTA−373813 receptor CXCR2 binding ligands CXCL1 to 7, R−BTA−373791 receptor CXCR1 binding CXCL6 and CXCL8 ligands, R−BTA−210991 basigin interactions, R−BTA−380108 chemokine receptors binding chemokines, R−BTA−445704 calcium binding caldesmon, and R−BTA−5669034 TNFs binding their physiological receptors. Furthermore, the expression trend of the DEGs in these pathways were also exploited. Moreover, the bta-miR-149-5p significantly (p < 0.01) downregulated the mRNA levels of adipogenic marker genes such as CCND2, KLF6, ACSL1, Cdk2, SCD, SIK2, and ZEB1 in bovine adipocytes. In conclusion, our results suggest that bta-miR-149-5p regulates lipid metabolism in bovine adipocytes. The results of this study provide a basis for studying the function and molecular mechanism of the bta-miR-149-5p in regulating bovine adipogenesis.

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“…Further analysis of pathway enrichment showed that PPAR signaling was upregulated significantly, and some metabolism-related pathways were regulated differentially ( Khan et al, 2019 , 2020a ; Chen et al, 2020 ). Among them, HSL and PLIN1 were significantly enriched during the regulation of lipolysis in adipocytes and in the AMPK signaling pathway, the adipocytokine signaling pathway and the PPAR signaling pathway, which are involved in the regulation of multiple fat metabolism pathways; therefore, HSL and plin1 were selected as important candidate marker genes for beef fat decomposition ( Junjvlieke et al, 2020 ; Khan et al, 2020b ). Combined with the gene network of fat metabolism constructed in this study, there are five fat metabolism pathways: the excitatory G protein-coupled receptor pathway, inhibitory G protein-coupled receptor pathway, Gq/PLC/PKC pathway, tyrosine kinase receptor pathway and JAK/STAT pathway.…”

Section: Discussionmentioning

confidence: 99%

A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

Liu

Bai

et al. 2021

Front. Genet.

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Fat metabolism is closely related to the economic characteristics of beef cattle. Therefore, regulating fat deposition and increasing intramuscular fat deposition are among the main goals of breeders. In this study, we aim to explore the regulatory role of CB1 gene on PPARγ2/PLIN1/HSL pathway in fat metabolism, and to further explore the differential expression of regulatory factors of this pathway in Shandong black cattle and Luxi cattle. In this study, CB1 overexpression stimulated lipid synthesis in adipocytes to some extent by increasing the levels of FASN and ACSL1. CB1 inhibitors reduce the lipid content in adipocytes and reduce the expression of GLUT1 and Insig1. In addition, overexpression of CB1 decreased the expression of PPARγ2 and led to an increase in PLIN1 expression and a decrease in HSL expression in adipocytes. We also found that the CB1/PPARγ2/PLIN1/HSL was differentially expressed in the different breeds of cattle and was involved in the regulation of fat metabolism, which affected the fatty acid content in the longissimus dorsi muscle of the two breeds. In short, CB1 participates in lipid metabolism by regulating HSL in the PPARγ2 and PLIN1 pathways, and improves lipid formation in adipocytes. In conclusion, CB1/PPARγ2/PLIN1/HSL pathway may be involved in the regulation of lipid metabolism.

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Effect of ELOVL6 on the lipid metabolism of bovine adipocytes

Cited by 31 publications

References 37 publications

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling

RNA-Seq Reveals Function of Bta-miR-149-5p in the Regulation of Bovine Adipocyte Differentiation

A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

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