Effect of Elevated Temperature on Cytotoxic Effector Cells

Singh, Vijay K.; Biswas, Soham; Pandey, Chandra M.; Agarwal, S. S.

doi:10.1159/000164029

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…Thus, in a first order approximation, the photochemical damage spectrum proposed initially by Liang et al19 for CHO cells can be assumed as universal. Photothermal damage takes place when, due to absorption, laser induced thermal loading drives the intracellular temperature up to the cytotoxic level (>43 °C) 20. At such temperatures irreversible protein denaturation takes place and the cell will die either by programmed cell death or immediately, due to trauma.…”

Section: Resultsmentioning

confidence: 99%

Quantum Dot‐Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells

Haro‐González

Ramsay

Maestro

et al. 2013

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Laser‐induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non‐localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 °C), thus, avoiding cell damage.

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Section: Resultsmentioning

confidence: 99%

Quantum Dot‐Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells

Haro‐González

Ramsay

Maestro

et al. 2013

Small

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“…In contrast with these results, some others have shown declining killing activity of NK cells to hyperthermia. In 1996 Singh.V showed reducing cytotoxic activity of NK cells at high temperature of 38.5°C [19] . Azocar J. et.al (1982) showed that heat-treated NK cells and K-562 target cells decreased cytotoxic activity of these cells at temperatures 38.5°C and 40°C [14] .…”

Section: Discussionmentioning

confidence: 91%

Analysis of Hyperthermia Effect on Peripheral Blood Natural Killer Cell Cytotoxicity against SW-872 Cell Line

Atashzar

Jalili

2015

GMJ

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Background: Natural killer (NK) cells are a type of cytotoxic lymphocyte. It is revealed that hyperthermia which is used in cancer treatment, increases natural killer cell activity. In this study, our aim was to analyze effects of in-vitro hyperthermia on human Natural Killer cytotoxicity against SW-872 cell line. Materials and Methods: In this study, we used SW-872 liposarcoma cell line as a target cell. Peripheral blood NK cells were isolated from normal individuals by MACS. NK cells were treated at 39°C for 1hr .The expression of CD69 on the surface of NK cells was examined by flow cytometry at different time points including 0, 6 and 12 hrs. NK cell cytotoxicity was measured by LDH assay 12hrs after co-culture with SW-872. The results were compared to the conditions that both NK cells and SW-872 cells were heat treated at 39°C for 12hrs. Results: Our results revealed that cell killing activity of NK cells treated with heating alone was increased 6hrs after heat treatment at 39°C compared with heat-treated NK-target cells co-culture. While in heat-treated NK-target cells co-culture the maximum cytotoxicity was observed 12hrs after heat treatment at 39°C. Conclusion: These results showed that thermotherapy could be a feasible method to stimulate immune response against tumor cells. [GMJ. 2015;4(3):75-81]

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“…Shen et al [16] showed that IL-2-activated cytotoxicities of nonadherent cells from adult blood and cord blood could be enhanced in the febrile range (^40°C), and that the activities were significantly decreased by exposure to heat for 60 min at 42°C. Singh et al [19] found that lymphokine-activated killer (LAK) cell cytotoxicity was significantly inhibited at 40°C incubation as compared with 37°C incubation for 5 days when natural killer (NK)-sensitive K562 cells were used as the target, or was not significantly changed at 40°C incubation when NK-resistant Raji cells were used.…”

Section: Discussionmentioning

confidence: 99%

Effect of Heat-Pretreatment on Interleukin-2-Activated Killer Cells for in vitro Purging

et al. 2000

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Objective: Lymphokine-activated killer activity is effective in purging residual malignant cells from autologous bone marrow (BM) or leukapheresis collections for peripheral blood progenitor cells (PBPC). In this study, the effect of additional heat pretreatment on IL-2-activated cytotoxicity was examined. Methods: Nonadherent mononuclear cells from peripheral blood (PB), PBPC collections or BM were exposed to the desired temperature for 1 h, and successively cultured for 7 days in the presence of 1,000 units/ml of recombinant human interleukin-2 (IL-2). Cytotoxicity of IL-2-activated cells was determined by flow cytometry, using PKH-26-labelled K-562 or Raji target cells, and the cell surface antigens were also analyzed. Results: IL-2-activated cells from all specimens showed a distinct generation of cytotoxic activities. The activities were significantly decreased by heat at 42°C, but not changed by heat pretreatment at 40°C. In the surface expression of IL-2-activated cells, the increase of CD56+ cells from PB, PBPC collections and the decrease of CD28 cells from PBPC collections were shown to be significant (p < 0.05), but these changes were not found when the cells were heat-pretreated at 42°C. Discussion: These results suggested that a tumor-killing therapeutic temperature (≧42°C) may lead to a disadvantage in posttransplantation immunity, and that this reduction of IL-2-activated cytotoxicity may partly be due to a lack of the enhancement of CD56+ cells or the reduction of CD28+ cells.

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Effect of Elevated Temperature on Cytotoxic Effector Cells

Cited by 5 publications

References 21 publications

Quantum Dot‐Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells

Quantum Dot‐Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells

Analysis of Hyperthermia Effect on Peripheral Blood Natural Killer Cell Cytotoxicity against SW-872 Cell Line

Effect of Heat-Pretreatment on Interleukin-2-Activated Killer Cells for in vitro Purging

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