Effect of electrolyzed water on enzyme activities of triticale malt during germination

Abstract

Triticale malt can be used as a source of enzymes or as a raw material for the production of functional foods. In this study, triticale malt was produced by soaking triticale seeds either in tap water (TW) or slightly acidic electrolyzed water (SAEW) and then rinsing with TW, SAEW, or alkaline electrolyzed water (AEW). We determined the length of the hypocotyl of triticale malt and the activities of a-amylase, phytase, proteases, and lipase during 4 days of germination. The electrolyzed water (EW) treatments promoted the growth of triticale malt. On the 4th day of germination, the hypocotyl length of triticale malt soaked in TW and watered with SAEW was 24.57% longer than that of triticale malt soaked and watered with TW. The a-amylase, phytase, acidic protease, and lipase activities of triticale malt soaked in SAEW and watered with AEW were high on the 4th germination day (0.11, 1.24 9 10 -4 , 0.62, and 0.51 units/mg protein, respectively). The main finding of this study is that the use of EW, especially during the soaking procedure, may be a promising way to obtain triticale malt with high enzyme activity for use in the production of functional foods.

Electrolyzed water: Effect on seed decontamination and seed quality

“…lycopersici) and also reduced the number of diseased fruits/plant, number of spots/fruit; and increased the number of healthy fruits/plant and total fruit yield/plant (Abbasi and Lazarovits, 2006) [63] . Yu and Liu, (2019) [64] found that electrolyzed water soaking improved the α-amylase activity, protease activity, phytase activity and lipase activity in triticale during seed germination.…”

Gas chromatography-mass spectrometry analysis of variations in the essential leaf oil of 6 Eucalyptus Species and allelopathy of mechanism 1,8-cineole

“…Previous studies have demonstrated the antibacterial potential of 1,8-cineole (SIMSEK & DUMAN, 2017). The germination of seeds is mainly related to amylase, lipase, and protease enzymes, which hydrolyze the starch, fat, and protein stored in the seeds to provide energy for germination (CENTENO et al, 2001;FASHUI et al, 2003;SIBIAN et al, 2017;yU & LIU, 2019). The starch content in the wheat seeds is high (KUMAR et al, 2018).…”

Tendencias recientes en aplicaciones de agua electrolizada: áreas de estudio y perspectivas

“…Diversos autores, al estudiar la capacidad del AEN para desinfectar las semillas de la alfalfa, el brócoli, el tomate, la lechuga y el arroz, encontraron que aumentaba el porcentaje de germinación y la calidad de las plantas en desarrollo(Abbasi & Lazarovitz, 2006;Kim, Feng, Kushad & Fan, 2006;Liu et al, 2013;Zhang et al, 2019;Guerra, Villalba, Contreras, Debasis & Sandoval, 2022). Los autoresYu & Liu (2019), concluyeron que al emplear el AEN en los pretratamientos, se incrementaba la actividad de la α-amilasa, de las proteasas, de las fitasas y las lipasas en el cereal triticale. Asimismo,Li et al (2018), indicaron que al aplicar el AEN a 40 mg/L durante rutina, que son los metabolitos secundarios generados durante el proceso germinativo y desarrollo de las plántulas.…”