Effect of electrolytes on proteins physisorption on ordered mesoporous silica materials

Salis, Andrea; Medda, Luca; Cugia, Francesca; Monduzzi, Maura

doi:10.1016/j.colsurfb.2015.04.068

Cited by 34 publications

(27 citation statements)

References 104 publications

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“…In phosphate buffer at pH 7.4 all functionalized MSNs display negative values of , being the  values increasingly negative according to the following sequence: -NH2 < -HAS < -HAM < -HAL. Comparing these values to those measured in water a significant adsorption of the H2PO4and HPO4 2anions at the solid surface can be suggested not only for MSN-NH2 (that is expected as a result of strong electrostatic interactions induced particularly by divalent HPO4 2anions), but also for HA-grafted MSNs (see  values in Table S1): in the latter case the adsorption of buffer anions is likely due to specific interactions originated by van der Waals (non-electrostatic) forces [80][81][82]. Turning the attention to the particles' size dH determined through DLS (see Fig.…”

Section: Figurementioning

confidence: 84%

Mesoporous silica nanoparticles functionalized with hyaluronic acid. Effect of the biopolymer chain length on cell internalization

Nairi¹,

Magnolia²,

Piludu³

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Mesoporous silica nanoparticles (MSNs) were functionalized with amino groups (MSN-NH) and then with hyaluronic acid, a biocompatible biopolymer which can be recognized by CD44 receptors in tumor cells, to obtain a targeting drug delivery system. To this purpose, three hyaluronic acid samples differing for the molecular weight, namely HA (8-15 kDa), HA (30-50 kDa) and HA (90-130 kDa), were used. The MSN-HA, MSN-HA, and MSN-HA materials were characterized through zeta potential and dynamic light scattering measurements at pH = 7.4 and T = 37 °C to simulate physiological conditions. While zeta potential showed an increasing negative value with the increase of the HA chain length, an anomalous value of the hydrodynamic diameter was observed for MSN-HA, which was smaller than that of MSN-HA and MSN-HA samples. The cellular uptake of MSN-HA samples on HeLa cells at 37 °C was studied by optical and electron microscopy. HA chain length affected significantly the cellular uptake that occurred at a higher extent for MSN-NH and MSN-HA than for MSN-HA and MSN-HA samples. Cellular uptake experiments carried out at 4 °C showed that the internalization process was inhibited for MSN-HA samples but not for MSN-NH. This suggests the occurrence of two different mechanisms of internalization. For MSN-NH the uptake is mainly driven by the attractive electrostatic interaction with membrane phospholipids, while MSN-HA internalization involves CD44 receptors overexpressed in HeLa cells.

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Section: Figurementioning

confidence: 84%

Mesoporous silica nanoparticles functionalized with hyaluronic acid. Effect of the biopolymer chain length on cell internalization

Nairi¹,

Magnolia²,

Piludu³

et al. 2018

Colloids and Surfaces B: Biointerfaces

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“…Ordered mesoporous silica (OMS) materials have widely been investigated for several applications. Since their discovery in the 1990s, 20 they have been used for catalysis, 21,22 biocatalysis, 23−26 adsorption, 27,28 and as drug delivery carriers. 29−32 The main goal of these materials is due to their large specific surface area and highly uniform pore size.…”

Section: Introductionmentioning

confidence: 99%

Glassy Carbon Electrodes Modified with Ordered Mesoporous Silica for the Electrochemical Detection of Cadmium Ions

et al. 2019

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Four different samples of ordered mesoporous silica powders (MCM-41 and SBA-15) and amino-functionalized mesoporous silica (MCM-41-NH 2 and SBA-15-NH 2 ) were used to prepare modified glassy carbon electrodes coated with ion-exchange polymer Nafion to be used for the electrochemical detection of Cd(II). The mesoporous silica samples were characterized through transmission electron microscopy, small-angle X-ray scattering, and N 2 -adsorption/desorption isotherms. The electrodes were characterized by using square wave anodic stripping voltammetry. The effect of pH and of the silica type on the electrodes’ response was investigated. The influence of amino functional groups grafted on the silica surface toward Cd(II) ion detection was also examined. The detection limits determined with the new silica-modified electrodes [between 0.36 and 1.68 μM Cd(II)] are slightly higher than those reported in the literature, but they are lower than those stipulated in the European legislation [45 μM Cd(II)] and, consequently, the electrodes could be successfully used to detect Cd(II) in aqueous solutions.

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“…54 From a physicochemical view point, the therapeutic can be adsorbed in the surface of mesoporous nanosilica forming both ionic interactions and hydrogen bonds. 55,56 The "burst effect" observed in the release of Dex from collagen alone suggests that Dex does not have strong interactions with the collagen or that diffusivity in the collagen network is high (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

ECM–oligourethene–silica hydrogels as a local drug release system of dexamethasone for stimulating macrophages

et al. 2017

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Effect of electrolytes on proteins physisorption on ordered mesoporous silica materials

Cited by 34 publications

References 104 publications

Mesoporous silica nanoparticles functionalized with hyaluronic acid. Effect of the biopolymer chain length on cell internalization

Mesoporous silica nanoparticles functionalized with hyaluronic acid. Effect of the biopolymer chain length on cell internalization

Glassy Carbon Electrodes Modified with Ordered Mesoporous Silica for the Electrochemical Detection of Cadmium Ions

ECM–oligourethene–silica hydrogels as a local drug release system of dexamethasone for stimulating macrophages

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