“…Biological trials use different cell lines, mostly, mesenchymal stem cell [48,49], MG63 osteoblast cells [50,51], mouse osteoblast cell line [52], NIH-3T3 murinefibroblast cell [38], and human CCD-18Co fibroblasts cell lines [53]. Cell viability is quantitatively analyzed via dimethylthiazol-diphenyl tetrazolium bromide (MTT) assay [19,54,55], which has been widely used for appraising cytotoxicity and proliferation. Cell proliferation is commonly observed by using a fluorescent microscope [56].…”