Effect of electrohydraulic and extracorporeal shock waves on gastrointestinal cancer cells and their response to cytotoxic agents.

Warlters, Andrea; Dl, Morris; Cameron-Strange, A; Lynch, William R.

doi:10.1136/gut.33.6.791

Cited by 10 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other mechanisms might be active as well since a slightly enhanced effect of another drug was noted even when it was added 24 hours after shock wave administration (Oosterhof et al 1989). A similar effect was found by another group, however in only one out of a number of cell lines (Warlters et al 1992). …”

Section: In Vitro Actionmentioning

confidence: 59%

Medical applications and bioeffects of extracorporeal shock waves

Delius¹

1994

Shock Waves

163

View full text Add to dashboard Cite

Abstract. Lithotripter shock waves are pressure pulses of microsecond duration with peak pressures of 35-120MPa fotlowed by a tensile wave. They are an established treatment modality for kidney and gallstone disease. Further applications are pancreatic and salivary stones, as well as delayed fracture healing. The latter are either on their way to become established treatments or are currently under investigation. Shock waves generate tissue damage as a side effect which has been extensively investigated in the kidney, the liver, and the gallbladder. The primary adverse effects are local destruction of blood vessels, bleedings, and formation of blood clots in vessels. Investigations on the mechanism of shock wave action revealed that lithotripters generate cavitation both in vitro and in vivo. An increase in tissue damage at higher pulse administration rates, and also at shock wave application with concomitant gas bubble injection suggested that cavitation is a major mechanism of tissue damage. Disturbances of the heart rhythm and excitation of nerves are further biological effects of shock waves; both are probably also mediated by cavitation. On the cellular level, shock waves induce damage to cell organelles; its extent is related to their energy density. They also cause a transient increase in membrane permeability which does not lead to celJ death. Administered either alone or in combination with drugs, shock waves have been shown to delay the growth of small animal tumors and even induce tumor remissions. While the role of cavitation in biological effects is widely accepted, the mechanism of stone fragmentation by shock waves is still controversial. Cavitation is detected around the stone and hyperbaric pressure suppresses fragmentation; yet major cracks are formed early before cavitation bubble collapse is observed. The latter has been regarded as evidence for a direct shock wave effect.

show abstract

Section: In Vitro Actionmentioning

confidence: 59%

Medical applications and bioeffects of extracorporeal shock waves

Delius¹

1994

Shock Waves

163

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…The in vitro effects of shock waves are due to a suppression of cell proliferation correlated with an apoptotic cell death process; moreover, HESW treatment has been shown to cause a transient increase in cell membrane permeability by opening micropores (such as electroporation), allowing higher intracellular drug concentration (12-16). This effect has been shown as being capable of increasing the cytotoxicity of various chemotherapeutic agents on different tumour types, allowing treatments with lower doses of drugs in vitro (17)(18)(19), in vivo in animal models (12,20) and in one patient with prostate cancer (21).This evidence led us to study the effects of HESW on OS cell lines and how to enhance cytotoxicity with a combined therapy with HESW and doxorubicin (DOXO) or methotrexate (MTX). These cytotoxic agents have been chosen since their well-known activity against OS and their routine use in clinical protocols for the treatment of OS patients (22)(23)(24)(25)(26)(27).…”

mentioning

confidence: 99%

High energy shock waves enhance the cytotoxic effect of doxorubicin and methotrexate to human osteosarcoma cell lines

Palmero

Berger²,

Venturi³

et al. 2006

Oncol Rep

View full text Add to dashboard Cite

Abstract. Despite recent advances, the prognosis of relapsed osteosarcoma patients remains very poor. Application of high energy shock waves may change the tumour cell growth and increase the cytotoxic effect of in vivo and in vitro chemotherapeutic agents. We studied the effect of their association with doxorubicin or methotrexate on three in vitro osteosarcoma cell lines. The effect of these combinations on SJSA-1, MG-63 and HOS human osteosarcoma cell lines were evaluated through incubation with doxorubicin or methotrexate and high energy shock wave treatment with 1000 shots at 0.22 mJ/mm 2 and an evaluation of the cell number, cell proliferation and apoptosis at days 1, 3 and 6 from the start of treatment. The combination of high energy shock waves and doxorubicin induced a statistically significant advantage in terms of a decrease in cell number on the SJSA-1 and HOS cell lines, a decrease of cell proliferation on all three cell lines and an increase of apoptosis only on the SJSA-1 cell line. The combination of high energy shock waves with methotrexate induced a decrease of the cell number only in the SJSA-1 and in the HOS cell lines, of the cell proliferation in the SJSA-1 and in the MG-63 cell lines, and an increase of apoptosis only on the SJSA-1 cell line. In conclusion, these experiments show an interesting effect of high energy shock waves on osteosarcoma cell lines, which could lead to future studies of the in vivo effects of high energy shock waves in the murine model as well. IntroductionOsteosarcoma (OS) is the most frequent bone tumour and is most common between the ages of 15 and 25. A high number of patients have a high-grade tumour, which prognosis has dramatically improved from 10-15% to 65-70% in the last 25 years, thanks to the use of adjuvant and neoadjuvant chemotherapy (1,2). Nevertheless, despite continuing scientific research into the treatment, the prognosis of the patients with OS in metastatic relapse, especially to bones (3), is still very poor; even with a recently concluded Italian and Scandinavian phase II protocol including high-dose chemotherapy the 3-year overall survival and the 3-year disease free-survival rates were only 20% and 12% respectively (4).The search of new treatments for improving the survival of the patients without increasing the collateral effects of the chemotherapy has led us to study new ways to increase the chemo-sensitivity of the OS cells.High energy shock waves (HESW) are routinely used in clinical practise in orthopaedic and in urology to treat a number of different diseases, such as urinary stones, non-unions of the bone fractures, tennis elbow, calcifying tendonitis, pseudoarthrosis and necrosis of the femoral head (5,6).In the past, many studies of HESW were conducted on different human and animal tumour cell lines, describing an alteration of the cell growth (7-11). The in vitro effects of shock waves are due to a suppression of cell proliferation correlated with an apoptotic cell death process; moreover, HESW treatment has been shown to cause a t...

show abstract

“…Several research groups have found some toxicity of shockwaves to tumours in animal models (Oosterhof et al, 1991;Russo et al, 1986;1987). Others have shown that shockwaves could enhance cytotoxicity when cells were shortly incubated with the drugs during shock wave exposure (Gambihler & Delius, 1992;Warlters et al, 1992). In previous works, we showed that the combination of conventional shockwaves with gas microbubbles could: (1) induce a blockade of the growth of human colon cells in culture and eradicate cell proliferation from solid colic tumours treated in vitro (Prat et al, 1991b); (2) potentiate the cytotoxicity of FUra in HT-29 cells (Prat et al, personal communication).…”

Section: Animalsmentioning

confidence: 99%

In vivo effects of cavitation alone or in combination with chemotherapy in a peritoneal carcinomatosis in the rat

Prat

Chapelon²,

Fadil³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

Summary Cavitation (volume oscillations and collapse of gas bubbles), as generated by a co-administration of shockwaves (SW) and microbubbles (SWB) Group, 1984;Calabresi & Chabner, 1990;Valeriote & Santelli, 1984) and only surgery is able to improve survival in less than 10% of the patients with liver metastasis (Nakamura et al., 1992;Registry of Hepatic Metastases, 1988). New therapeutic instruments are thus clearly needed to improve the prognosis of metastasis from bowel malignancies. Despite disappointing results from radiotherapy and conventional hyperthermia, physical methods may be interesting as an alternative approach to, or in combination with, chemotherapy. Biological effects of acoustic waves, like high intensity ultrasound and shock waves have been studied for many years (Flynn, 1964;Church & Miller, 1983); while direct cytotoxic effects may play a minor role, several studies have shown that damage is caused to microvessels and endothelial cells, causing vascular disruption as well as the production of free radicals, leading to hypoxia and indirect toxicity to the affected tissue (Miller, 1987). It has also been demonstrated that the cytotoxicity of shock waves was obtained mostly through acoustic cavitation, which is the transitory volume oscillations of gas microbubbles induced by rapidly varying pressure waves, eventually resulting in the collapse of bubbles (Dear et al., 1988). When a bubble collapses near an interface with a cell membrane, such dramatic damage is inflicted to the cell as to induce cell death (Delius et al., 1989;Miller, 1987;Miller et al., 1991). However, the therapeutic potentialities of cavitation have so far remained confined to in vitro experimental studies and have not evolved toward clinical applications for two reasons: (1) ultrasound was not able to induce cytotoxicity unless generated in vitro in specific experimental conditions (Church & Miller, 1983); (2) (Prat et al., 199 la). Further experiments with HT-29 cells in suspension and viable rat colon peritoneal metastases treated in vitro showed that cavitation could hinder cell proliferation and induce complete tissue necrosis (Prat et al., 1991b). In a more recent study (Prat et al., personal communication), the cytotoxicity of FUra to HT-29 cells was greatly enhanced by a preliminary exposure of the cells to cavitation, through potentially synergistic mechanisms.In the present study, we aimed at investigating the relevance of cavitation to the treatment of a disseminated digestive tumour in vivo. Materials and methodsCells DHD K12 PROb cells (a gift from Pr Martin, INSERM U252, Dijon, France) originated from a dimethyl-hydrazineinduced rat colon carcinoma were cultured in Dulbecco's modified Eagle's medium supplemented with 8% fetal calf serum under 8% CO2. The cells were cultured to confluence in 75 cm2 flasks and detached after 12 days of culture by 2.5/1000 trypsin/0.2/1000 EDTA. Cell viability was assessed before each use by trypan blue exclusion; it was always over 90%. AnimalsMale and female BD IX rats, syng...

show abstract

Effect of electrohydraulic and extracorporeal shock waves on gastrointestinal cancer cells and their response to cytotoxic agents.

Cited by 10 publications

References 11 publications

Medical applications and bioeffects of extracorporeal shock waves

Medical applications and bioeffects of extracorporeal shock waves

High energy shock waves enhance the cytotoxic effect of doxorubicin and methotrexate to human osteosarcoma cell lines

In vivo effects of cavitation alone or in combination with chemotherapy in a peritoneal carcinomatosis in the rat

Contact Info

Product

Resources

About