Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase II in spinal cord injury rats

Min, You-jiang; Ding, Li-Li-Qiang; Cheng, Lihong; Xiao, Weiping; He, Xing-wei; Zhang, Hui; Min, Zhi-yun; Pei, Jia

doi:10.4103/1673-5374.200811

Cited by 9 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rats were fed with standard fodder and with food and water freely available in a controlled environment with a constant temperature of 20~25°C and a 12/12-hr light/dark cycle. 28 Following a 3-day adaptation, all rats were begun to experiment. 28 All procedures were conducted in accordance with guidelines reviewed and approved by the Institutional Animal Care and Use Committee of Jiangxi University of Traditional Chinese Medicine, China 28 .…”

Section: Experimental Animalsmentioning

confidence: 99%

“…28 Following a 3-day adaptation, all rats were begun to experiment. 28 All procedures were conducted in accordance with guidelines reviewed and approved by the Institutional Animal Care and Use Committee of Jiangxi University of Traditional Chinese Medicine, China 28 . Female animals are routinely used in SCI studies because female animals allow for easier manual expression of bladders after SCI, less urinary tract infection, and less mortality.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…The BBB Scale locomotor tests of all rats were blindly performed before treatment and on the 7th and 14th day when treatment began by 2 trained observers lacking knowledge of the experimental groups, according to a method published previously. 28 Before assessment, the bladder of each rat was emptied to avoid abnormal behavior caused by a full bladder. Briefly, rats were placed in an open field and observed for 3 mins.…”

Section: Behavioral Assessmentsmentioning

confidence: 99%

“…Western blot analysis was performed as described previously with minor modifications. 28 All spinal cord tissues obtained in each group were homogenized in lysis buffer (JRDUN Biotechnology, Shanghai, China). Equal amounts of proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then the resolved proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA).…”

Section: Western Blot Assaymentioning

confidence: 99%

“…24,25 Previous studies indicate that acupuncture could treat SCI by regulating the gene and protein expression of Nogo-A, NgR and/or RhoA, ROCK II. [26][27][28] Does EA influence the Nogo/NgR and Rho/ROCK signal pathway after SCI? How does EA treat SCI by affecting the Nogo/ NgR and Rho/ROCK signaling pathway?…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

<p>Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway</p>

Xiao¹,

Ding²,

Min³

et al. 2019

NDT

Self Cite

View full text Add to dashboard Cite

To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI. Methods: Allen's method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods. Results: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632 treatment alone (P < 0.01 or <0.05). Conclusion: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.

show abstract

Section: Experimental Animalsmentioning

confidence: 99%