Effect of Electric-Field Intensity on Electropermeabilization and Electrosensitmty of Various Tumor-Cell Lines<i>In Vitro</i>

Ĉemazˆr, Maja; Jarm, Tomaž; Miklavčič, Damijan; Lebar, Alenka Maček; Ihan, Alojz; Kopitar, NataŜA Andreja; Serša, Gregor

doi:10.3109/15368379809022571

Cited by 66 publications

(44 citation statements)

References 16 publications

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“…Our in vitro results demonstrate that endothelial cells are electroporated at the same field intensity previously shown to be effective for tumour cytotoxicity in vivo (Sersa et al, 1995;Miklavcic et al, 1998). However, compared to the tumour cells in vitro they appeared to be slightly more resistant to electric pulses (Cemazar et al, 1998c). The reasons for this are currently unclear.…”

Section: Discussioncontrasting

confidence: 42%

Electroporation of human microvascular endothelial cells: evidence for an anti-vascular mechanism of electrochemotherapy

et al. 2001

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Recent studies have indicated that the antitumour effectiveness of electrochemotherapy, a combination of chemotherapeutic drugs with application of high voltage electric pulses applied to the tumour nodule (electroporation), result in a significant reduction in tumour blood flow and may therefore be mediated by an anti-vascular mechanism. The aim of this study was to evaluate the cytotoxicity of electroporation with bleomycin or cisplatin on cultured human microvascular endothelial cells (HMEC-1). The sensitivity of HMEC-1 cells to a 5 min treatment by electroporation with bleomycin or cisplatin (8 electric pulses, pulse duration 100 μs, frequency 1 Hz, electric field intensity 1400 V cm –1 ) was compared to the sensitivity of cells treated continuously for 3 days with drugs alone. HMEC-1 cells were moderately sensitive to continuous exposure to cisplatin, but showed greater sensitivity to bleomycin. Combination of a 5 min drug exposure with electric pulses increased cytotoxicity of cisplatin by ∼10-fold for cisplatin and ∼5000-fold for bleomycin. The electroporation of HMEC-1 cells with bleomycin for a 5 min exposure was ∼250-fold better than a continuous exposure to the drug alone. The results of this study indicate that the anti-tumour action of electrochemotherapy is likely to be due, in part, to the highly sensitive response of vascular endothelial cells. Further studies are necessary to identify the determinants of endothelial response and its relationship to the anti-vascular action of electrochemotherapy in vivo. © 2001 Cancer Research Campaign http://www.bjcancer.com

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Section: Discussioncontrasting

confidence: 42%

Electroporation of human microvascular endothelial cells: evidence for an anti-vascular mechanism of electrochemotherapy

et al. 2001

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“…As we and others have shown that different intrinsic sensitivities to electroporation exist between different cell types in vitro, this might also be the case in solid tumors in vivo. 37,38 It has already been shown that electroporation, in addition to the direct effect on cell membranes, also affects the cytoskeleton and chromosomal DNA, and therefore might trigger different cellular processes leading to apoptosis or necrosis. 39,40 Another factor, which should be taken into account for gene delivery in vivo, is the amount of hypoxia (low oxygen ) present in the solid tumors.…”

Section: Discussionmentioning

confidence: 99%

Effective gene transfer to solid tumors using different nonviral gene delivery techniques: Electroporation, liposomes, and integrin-targeted vector

et al. 2002

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In this study, we measured transfection efficiency in vitro and in vivo using the following nonviral approaches of gene delivery: injection of plasmid DNA, electroporation -assisted, liposome -enhanced, and integrin -targeted gene delivery, as well as the combination of these methods. Four histologically different tumor models were transfected with a plasmid encoding the green fluorescent protein ( GFP ) ( B16 mouse melanoma, P22 rat carcinosarcoma, SaF mouse sarcoma, and T24 human bladder carcinoma ) using adherent cells, dense cell suspensions, and solid tumors. Emphasis was placed on different electroporation conditions to optimise the duration and amplitude of the electric pulses, as well as on different DNA concentrations for effective gene delivery. In addition, transfection efficiency was correlated with cell density of the tumors. The major in vivo findings were: ( a ) electroporationassisted gene delivery with plasmid DNA, employing long electric pulses with low amplitude, yielded significantly better GFP expression than short electric pulses with high amplitude; ( b ) electroporation combined with liposome -DNA complexes yielded the highest percentage of transfected tumor area in B16F1 tumor ( 6% ); ( c ) transfection efficiency of electroporation -assisted plasmid DNA delivery was dependent on tumor type; ( d ) integrin -targeted vector, alone or combined with electroporation, was largely ineffective. In conclusion, our results demonstrate that some nonviral methods of gene delivery are feasible and efficient in transfecting solid tumors. Therefore, this makes nonviral methods attractive for further development.

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“…11,[17][18][19]24,25,[27][28][29][30][31][32][33][34][35][36][37][38] To validate the correlation, we compared experimental data to predictions of electroporation conditions that yield 50% viability, since this criterion is often used as a 'rule of thumb' to crudely identify optimal laboratory transfection conditions. [1][2][3][4] Overall, predictions for 50% viability were within an average of 29 ± 28% of experimentally determined values (eg Figure 2a,c).…”

Section: Mammalian Viability Predictionsmentioning

confidence: 99%

Prediction and optimization of gene transfection and drug delivery by electroporation

Canatella

Prausnitz

2001

Gene Ther

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Effect of Electric-Field Intensity on Electropermeabilization and Electrosensitmty of Various Tumor-Cell LinesIn Vitro

Cited by 66 publications

References 16 publications

Electroporation of human microvascular endothelial cells: evidence for an anti-vascular mechanism of electrochemotherapy

Electroporation of human microvascular endothelial cells: evidence for an anti-vascular mechanism of electrochemotherapy

Effective gene transfer to solid tumors using different nonviral gene delivery techniques: Electroporation, liposomes, and integrin-targeted vector

Prediction and optimization of gene transfection and drug delivery by electroporation

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