Effect of Ejaculation Intervals on Semen Quality in Cats.

Tanaka, Akihiro; Kuwabara, Shigeru; Takagi, Yuuko; Nakagawa, Kiyoshi; Fujimoto, Youko; Murai, Mayumi; Tsutsui, Toshihiko

doi:10.1292/jvms.62.1157

Cited by 34 publications

(39 citation statements)

References 8 publications

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“…Semen collection and examination of semen quality: Semen for AI was collected with an artificial vagina (AV) for cats, as we previously reported [9].…”

Section: Animalsmentioning

confidence: 99%

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Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

Tsutsui

Tanaka

Takagi

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. The sperm count required were investigated to obtain a conception rate of 80% by unilateral intrauterine insemination (UIUI) of fresh semen in cats. The conception rates obtained by insemination before and after ovulation were also examined. Thirty-six female cats aged 1-7 years were used in the experiments, and the number of experimental cases was 44. Seven male cats aged 2-12 years from which semen could be collected by the artificial vagina method were used. In artificial insemination, 100 iu × 2 or 250 iu of hCG was administered on days 2-4 of estrus, and sperm were introduced into the uterine horn with a greater number of ovulations (or mature follicles) 15, 20 and 30 hr after hCG administration by laparotomy. The inseminated sperm counts were 2 × 10 6 (Exp. 1), 4 × 10 6 (Exp. 2), and 8 × 10 6 (Exp. 3). As a result, ovulation was induced in 42 of 44 cases (induction rate: 95.5%) regardless of the dosage of hCG. Conception was obtained by UIUI in two of 16 animals (conception rate: 12.5%) in the Exp. 1, five of 16 animals (31.3%) in Exp. 2, and eight of 10 animals (80.0%) in Exp. 3. Regarding the relationship between the ovulation state at insemination and conception, the conception rate obtained by insemination before ovulation was clearly higher than that obtained by insemination after ovulation (p<0.05). Regarding the number of kits compared to the number of ovulations on the inseminated side, the percentages of cases in which the number of kits exceeded the number of ovulations on the inseminated side were similar in all groups inseminated with a different number of sperm. It is therefore necessary to investigate conception rates obtained by bilateral insemination to increase the fertility rate. Based on the above findings, it was shown that the sperm count required for fertilization by UIUI is 8 × 10 6 . KEY WORDS: feline, fresh semen, intrauterine insemination.

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“…Semen collection and examination of semen quality: Semen for AI was collected with an artificial vagina (AV) for cats, as we previously reported [9].…”

Section: Animalsmentioning

confidence: 99%

“…The semen was evaluated by a general test for semen quality as we previously reported [9]. The semen specimens with a sperm motility of 80% or greater were used in AI.…”

Section: Animalsmentioning

confidence: 99%

Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

Tsutsui

Tanaka

Takagi

et al. 2000

The Journal of Veterinary Medical Science

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“…Semen collection and examination of semen quality: Semen was collected using an AV for cats that was previously reported [5].…”

Section: Animalsmentioning

confidence: 99%

“…We previously performed intravaginal insemination (IVI) of fresh semen and clarified that a number of sperms, 80 × 10 6 , are required for fertilization [6]. This sperm count is equal to the number of sperm in two ejaculations in cats [5]. We also clarified the sperm count required for fertilization by unilateral intrauterine horn insemination (UIUI) of fresh semen to be 8 × 10 6 in cats, which is 1/10 of that by IVI [7].…”

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confidence: 99%

“…Male cats were individually kept in cages measuring 60 × 90 × 120 (h) cm, and female cats were kept in a group in a room measuring 4 × 7 m. The animals were supplied dry food (Hill's Feline Maintenance, U.S.A.) and drinking water ad libitum. Females were placed with males every day in the morning and evening to observe estrus.Semen collection and examination of semen quality: Semen was collected using an AV for cats that was previously reported [5].While a male cat mounted an estrous female cat and thrust, the penis was advanced into an AV held between the forefinger and middle finger, and ejaculation was induced. Using…”

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Unilateral Intrauterine Horn Insemination of Frozen Semen in Cats.

Tsutsui

Tanaka

Takagi

et al. 2000

The Journal of Veterinary Medical Science

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ABSTRACT. Frozen feline semen was prepared using two types of extenders, egg yolk Tris-fructose citric acid (EYT-FC) and egg yolk sodium citrate solution (EYC), and the semen qualities after thawing and the conception rates obtained by unilateral intrauterine horn insemination (UIUI) were investigated. Cats used in the experiment were six males and 11 females aged 2-12 years (the number of experimental cases was 17). For preparation of frozen semen, semen collected by the artificial vagina method was adjusted to 1 × 10 8 sperm/ml and 7% glycerol, put in 250 µl straws, and then frozen using a cell freezer. The mean sperm motility after thawing was 30.0 ± 9.7 (SE) % in the semen prepared with EYT-FC and 30.0 ± 3.3% in the semen prepared with EYC. Four of seven animals were fertilized by UIUI using two straws in both extenders, and the conception rate was 57.1%. The mean ratios of number of kits to the number of ovulations in the inseminated side were 61.1 ± 24.5% and 30.5 ± 3.4% for EYT-FC and EYC, respectively, showing that the ratio tended to be higher in the semen prepared with EYT-FC. The above findings, comparing the two extenders for preparation of frozen feline semen, showed that EYT-FC is slightly superior to EYC. To increase conception and fertility rates, it may be important to increase the sperm count for insemination and to inseminate both uterine horns. KEY WORDS: feline, frozen semen, intrauterine insemination, straw method.J. Vet. Med. Sci. 62(12): 1247-1251 Feline artificial insemination (AI) using frozen semen has been reported only by Platz et al. in 1978 [3]. In their study, 50-100 × 10 6 viable sperms were inseminated into the vagina. Although only six of 56 animals were fertilized, obtaining a conception rate of 10.7%, these were the first cases of fertilization by frozen semen in cats. We previously performed intravaginal insemination (IVI) of fresh semen and clarified that a number of sperms, 80 × 10 6 , are required for fertilization [6]. This sperm count is equal to the number of sperm in two ejaculations in cats [5]. We also clarified the sperm count required for fertilization by unilateral intrauterine horn insemination (UIUI) of fresh semen to be 8 × 10 6 in cats, which is 1/10 of that by IVI [7]. Freezing markedly reduces motility and viability of sperms. Accordingly, it is important to develop a technique of fertilizaiton using a small number of sperm. Therefore, intrauterine insemination (IUI) may be appropriate for feline AI using frozen semen rather than IVI.The method using frozen feline semen reported by Platz et al. was the pellet method, which may pose difficulties for storage and use [3]. Therefore, we investigated freezing of feline semen by the straw method.Platz et al. used egg yolk lactose as the extender for frozen feline semen [3]. However, the semen qualities after thawing were not reported, and thus, we could not evaluate the extender used in their study. Therefore, we prepared frozen feline semen using egg yolk fructose citric acid (EYT-FC) [6,7], which we use as an e...

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Assisted Reproduction in the Male Cat

Johnson

2018

Veterinary Clinics of North America: Small Animal Practice

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Effect of Ejaculation Intervals on Semen Quality in Cats.

Cited by 34 publications

References 8 publications

Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

Unilateral Intrauterine Horn Insemination of Fresh Semen in Cats.

Unilateral Intrauterine Horn Insemination of Frozen Semen in Cats.

Assisted Reproduction in the Male Cat

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