Effect of Egg Yolk on Cryopreservation of Rhesus Monkey Ejaculated and Epididymal Sperm

Dong, Qiaoxiang; VandeVoort, Catherine A.

doi:10.2164/jandrol.108.006395

Cited by 14 publications

(10 citation statements)

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“…However, similar to results achieved with ram [26] and rhesus monkey [27] spermatozoa, the post-thaw motility of sperm samples did not decrease when the EY concentration was lowered. This detrimental effect on SV could be related to either the largest proportion of lactose in the extender or the poor cryoprotective ability of the lowered EY concentration with respect to the lactose proportion.…”

Section: Discussionsupporting

confidence: 82%

Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl β-Cyclodextrin or Cholesterol-loaded Cyclodextrin

et al. 2014

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Egg yolk (EY) and glycerol are common constituents of extenders used for sperm cryopreservation. It has been demonstrated that using cholesterol-loaded cyclodextrins (CLC) improves sperm cryosurvival in several species. However, standard freezing extenders might not be the most appropriate for CLC-treated sperm. This study evaluated the EY and glycerol requirements for freezing CLC-treated boar spermatozoa. Semen samples from 34 ejaculates coming from 4 boars were used. Each ejaculate was split into three aliquots: one was used untreated (control), and the other two were treated with 1 mg of CLC or methyl-β-cyclodextrin/120 × 106 sperm for 15 min at 22 C prior to cryopreservation. Our results indicated that reducing the concentration of EY was detrimental for sperm viability after thawing (31.57 ± 2 vs. 19.89% ± 2 for 20 and 10% EY, respectively; P <0.05), even in semen treated with CLC. On the other hand, it was observed that the traditional concentration of glycerol (3%) was not the appropriate for freezing CLC-treated sperm (61.10 ± 3 vs. 47.87% ± 3 viable sperm for control and CLC-treated sperm, respectively; P <0.05). Thus, CLC-treated sperm showed a higher tolerance to high glycerol concentrations (5%) in terms of sperm viability (59.19% ± 3) than non-treated sperm (45.58% ± 3; P<0.05). Therefore, it could be necessary to modify the freezing extenders for CLC-treated sperm. Nevertheless, additional studies will be needed to evaluate alternative cryoprotectants and to determine the effect of high glycerol concentrations on sperm functionality.

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Section: Discussionsupporting

confidence: 82%

Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl β-Cyclodextrin or Cholesterol-loaded Cyclodextrin

et al. 2014

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“…Though the exact means by which egg yolk helps sperm survive the cryopreservation process is unknown, it has generally been assumed that egg yolk stabilizes the sperm plasma membrane either directly, by forming a protective film [22] or replacing lost phospholipids [11,12,30], or indirectly by reducing the deleterious effect of seminal plasma proteins on the sperm membrane [2]. Yet, in the rhesus, a direct association of egg yolk with the sperm membrane seems to be the more likely explanation as egg yolk has been found to be equally necessary for protecting epididymal sperm as it is for ejaculated sperm [7]. …”

Section: Discussionmentioning

confidence: 99%

Interactions among pre-cooling, cryoprotectant, cooling, and thawing for sperm cryopreservation in rhesus monkeys

Dong¹,

Hill²,

VandeVoort³

2009

Cryobiology

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The present study evaluated the interactions among pre-cooling, cryoprotectant, cooling, and thawing for rhesus monkey sperm using a four-way factorial design. Specifically, pre-cooling and thawing were evaluated for two conditions: slow vs. fast. Cooling was evaluated at four rates of 5, 29, 200, and 400 °C/min. The types of cryoprotectant involved combinations of egg yolk and glycerol, egg yolk and ethylene glycol, and egg yolk alone without permeable cryoprotectants or buffer alone with glycerol but without egg yolk. Our findings showed strong interactions among cryoprotectants, cooling, and thawing rates, but not pre-cooling rate, on post-thaw motility and forward progression. The optimal combination of cooling and thawing for maximum post-thaw survival depended on the types of cryoprotectant. When glycerol was used as a permeable cryoprotectant in the presence of egg yolk, slow thawing yielded similar success as fast thawing in some males. However, when glycerol was replaced with ethylene glycol for the same treatment, post-thaw motility was significantly lower in samples that were thawed slowly than those that were thawed rapidly. In the absence of permeable cryoprotectant but the presence of egg yolk, fast cooling was always favorable. On the contrary, in the absence of egg yolk but the presence of permeable cryoprotectant (glycerol), post-thaw motility was significantly reduced especially when samples were thawed slowly. Generally, fast thawing was superior to slow thawing regardless of the types of cryoprotectant or cooling rates, and glycerol in the presence of egg yolk yielded the highest post-thaw motility in all treatment groups.

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“…Our results indicated that reducing the concentration of EY from 20 to 10% in a lactose-based diluent was detrimental for SV after thawing in all the sperm samples, even those treated with CLC prior freezing. However, and similarly to results achieved with ram (Marco-Jiménez et al, 2004) and rhesus monkey (Dong and Vandevoort, 2009) spermatozoa, the post-thaw motility of sperm samples did not decrease when the EY concentration was lowered. This detrimental effect in SV could be related to either the largest proportion of lactose in the extender or the poor cryoprotective ability of the lowered EY concentration with respect to the lactose proportion.…”

Section: Discussionsupporting

confidence: 81%

Treating boar sperm with cholesterol-loaded cyclodextrins or cyclodextrins prior to cryopreservation: effects on post-thaw in vitro sperm quality of sperm cryopreserved in different freezing extenders.

Torres¹

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ACKNOWLEDGEMENTSA mi directora de tesis, la Dra. Eva Mocé, por toda la dedicación, la orientación y por todo lo aprendido a lo largo de estos años.A mi "compi" Cristina Tomás, porque después de ella no ha habido compi que la supere, por todos los buenos ratos que hemos pasado juntas, por todas las comidas a contrarreloj (maldito timer) y porque siempre nos quedará nuestro rinconcito a 5 ºC… A Mar y la Gwen porque son tan grandes como nuestro despacho, a Carmela por los ratos de confesiones y risas, a Bego, Ire, Aran, Sofi, Alba, Paula, Mariam y a todos los compañeros del CITA por haber hecho que mi paso por el centro sea uno de mis mejores recuerdos… espero que nunca terminen las cerveCITAs.A todos las personas que conocí durante mi estancia en Murcia, en especial a Marta Hernández, por hacerme ver la luz tras el túnel de la citometría y al Dr Jordi Roca por toda su paciencia y dedicación. A las granjas Los Golliznos y El Toscal y al Centro de inseminación CIAR-GrupoArcoIris por facilitarnos los eyaculados necesarios para los experimentos. A Chori y compañía, los verdaderos protagonistas.A Mon y Ros, a los Dalmatillas de veterinaria, porque sé que siempre estarán ahí, en especial a Dave y Pati, my personal reviewers… Y, por supuesto, a mi familia, en especial a mis padres, a mi hermana y a Molli, por la insistencia, por creer en mi más que nadie (más que yo), por darme el apoyo necesario para no abandonar y conseguir mi objetivo. A Lourdes, Abelardo y resto de Almendraditos, porque un plato estrella y un flan siempre te ayudan a remontar. A Ángel (¿lo sabes o qué?), a Kachap por estar "literalmente" pegado a mi mientras esta tesis tomaba forma y a Garban, porque al final ha sido él quién que me ha obligado a dar el último empujón.Gracias a todos los que habéis ayudado a que esta tesis haya sido posible.

show abstract

Effect of Egg Yolk on Cryopreservation of Rhesus Monkey Ejaculated and Epididymal Sperm

Cited by 14 publications

References 50 publications

Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl β-Cyclodextrin or Cholesterol-loaded Cyclodextrin

Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl β-Cyclodextrin or Cholesterol-loaded Cyclodextrin

Interactions among pre-cooling, cryoprotectant, cooling, and thawing for sperm cryopreservation in rhesus monkeys

Treating boar sperm with cholesterol-loaded cyclodextrins or cyclodextrins prior to cryopreservation: effects on post-thaw in vitro sperm quality of sperm cryopreserved in different freezing extenders.

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