Effect of egg turning and incubation time on carbonic anhydrase gene expression in the blastoderm of the Japanese quail (<b><i>Coturnix c. japonica</i></b>)

Winter, Patricia de; Sugden, David; Baggott, Glenn K.

doi:10.1080/00071660802294558

Cited by 6 publications

(4 citation statements)

References 27 publications

(38 reference statements)

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“…The identification of multiple reference genes has been performed in various animal species including pig [23], cattle [24,25,26], dog [27] and avian species (in particular, chicken) [28,29,30,31]. Concerning quails, although a RT-qPCR normalization based on several genes was already published, this study was limited to an embryonic tissue, the blastoderm [32]. Thus, there is a lack of information regarding suitable reference genes in adult quails.…”

Section: Introductionmentioning

confidence: 99%

Identification of Reference Genes for Quantitative Gene Expression Studies in Three Tissues of Japanese Quail

Carvalho

Couroussé

Crochet

et al. 2019

Genes

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RT-qPCR is the gold standard for candidate gene expression analysis. However, the interpretation of RT-qPCR results depends on the proper use of internal controls, i.e., reference genes. Japanese quail is an agronomic species also used as a laboratory model, but little is known about RT-qPCR reference genes for this species. Thus, we investigated 10 putative reference genes (ACTB, GAPDH, PGK1, RPS7, RPS8, RPL19, RPL32, SDHA, TBP and YWHAZ) in three different female and male quail tissues (liver, brain and pectoral muscle). Gene expression stability was evaluated with three different algorithms: geNorm, NormFinder and BestKeeper. For each tissue, a suitable set of reference genes was defined and validated by a differential analysis of gene expression between females and males (CCNH in brain and RPL19 in pectoral muscle). Collectively, our study led to the identification of suitable reference genes in liver, brain and pectoral muscle for Japanese quail, along with recommendations for the identification of reference gene sets for this species.

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Section: Introductionmentioning

confidence: 99%

Identification of Reference Genes for Quantitative Gene Expression Studies in Three Tissues of Japanese Quail

Carvalho

Couroussé

Crochet

et al. 2019

Genes

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“…The tools available to compare those less genetically amenable bird species are highly limited, one problem being the lack of sequence information to design PCR-primers. To date, avian reference genes have been published only for the domestic chicken [33], [34], great tit ( Parus major ) [32], Japanese quail ( Coturnix c. japonica ) [35] and domestic pigeon ( Columba livia f. domestica ) [36].…”

Section: Introductionmentioning

confidence: 99%

Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species

et al. 2014

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Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data. In recent years, an increasing number of avian species other than the chicken has been investigated molecularly, highlighting the need for an experimentally validated pan-avian primer set for reference genes. Here we report testing a set for 14 candidate reference genes (18S, ABL, GAPDH, GUSB, HMBS, HPRT, PGK1, RPL13, RPL19, RPS7, SDHA, TFRC, VIM, YWHAZ) on different tissues of the mallard (Anas platyrhynchos), domestic chicken (Gallus gallus domesticus), common crane (Grus grus), white-tailed eagle (Haliaeetus albicilla), domestic turkey (Meleagris gallopavo f. domestica), cockatiel (Nymphicus hollandicus), Humboldt penguin (Sphenicus humboldti), ostrich (Struthio camelus) and zebra finch (Taeniopygia guttata), spanning a broad range of the phylogenetic tree of birds. Primer pairs for six to 11 genes were successfully established for each of the nine species. As a proof of principle, we analyzed expression levels of 10 candidate reference genes as well as FOXP2 and the immediate early genes, EGR1 and CFOS, known to be rapidly induced by singing in the avian basal ganglia. We extracted RNA from microbiopsies of the striatal song nucleus Area X of adult male zebra finches after they had sang or remained silent. Using three different statistical algorithms, we identified five genes (18S, PGK1, RPS7, TFRC, YWHAZ) that were stably expressed within each group and also between the singing and silent conditions, establishing them as suitable reference genes. In conclusion, the newly developed pan-avian primer set allows accurate normalization and quantification of gene expression levels in multiple avian species.

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“…In order to identify the specific isoenzyme responsible for the histochemical staining, we compared the distributional patterns of CA activity with those produced by immunohistochemistry. However, despite evidence for CA reactive sites, the present immunohistochemical findings failed to visualize the occurrence, in the lingual gland secretory cells, of the cytosolic CAII isoenzyme, known to be widely expressed in avian tissues 17,18 . The reliability of such a result is based on the cross-reactivity that the anti-chicken CAII showed in quail tissues by the Western blotting analysis (Figure 2) as well as on recent works which documented 100% similarity between quail and chicken amplicons for CAII 18 .…”

Section: Discussionmentioning

confidence: 39%

Carbonic anhydrase in minor salivary glands of quail: histochemistry versus immunohistochemistry

Gabrielli

Tomassoni

2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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Studies on the mechanisms of saliva secretion have indicated that carbonic anhydrase (CA) is expressed in mammalian salivary glands. The enzyme is present in the saliva as the only known secretory isoenzyme, CAVI; its activity has been related to the modulation of taste and caries development. Unlike mammals, in birds, saliva is produced by the so-called minor salivary glands, mostly concentrated in the tongue. The involvement of CA has never been explored in avian salivary secretion. Thus, we aimed here to ascertain the enzyme occurrence in the quail lingual glands by a parallel investigation of the distributional patterns of CA activity sites, as visualized by histochemistry, and the immunohistochemical patterns of cytosolic CAII and secretory CAVI. The comparative evaluation of our findings does not rule out that some CA isoforms, associated to basolateral borders of the secretory cells and antigenically different from cytosolic CAII and secretory CAVI, may be involved in the salivary secretion in the quail lingual glands.

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Effect of egg turning and incubation time on carbonic anhydrase gene expression in the blastoderm of the Japanese quail (Coturnix c. japonica)

Abstract: 1. The gene expression of carbonic anhydrase,

Cited by 6 publications

References 27 publications

Identification of Reference Genes for Quantitative Gene Expression Studies in Three Tissues of Japanese Quail

Identification of Reference Genes for Quantitative Gene Expression Studies in Three Tissues of Japanese Quail

Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species

Carbonic anhydrase in minor salivary glands of quail: histochemistry versus immunohistochemistry

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