Effect of EGF on rat parotid gland secretory function

Purushotham, Karnam R.; Zelles, T; Blazsek, J.; Wang, Paoli; Paul, George A.; Kerr, Micah; Humphreys‐Beher, Michael G.

doi:10.1016/0742-8413(94)00083-m

Cited by 6 publications

(8 citation statements)

References 23 publications

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“…Incubation of parotid acinar cells with sodium orthovanadate not only prevented an increase in phosphatase activity measured by pNPP hydrolysis but also inhibited the amylase release that is stimulated by P-adrenergic agonist (Purushotham et al, 1995a). There are at least four proteins-identified with Mr 40, 45, 70, and 95 kDa-that undergo increased phosphorylations on tyrosine residues upon stimulation by orthovanadate.…”

Section: Mediation Of Secretionmentioning

confidence: 99%

“…The chronic activation of the phosphotyrosine second messenger pathway observed in human salivary neoplasms does appear to affect the transcription rate and subsequent presence of amylase activity in tumor cell lysates (Morley and Hodes, 1988). Short-term growth induced by EGF increases transcription and release of amylase from the gland into saliva (Purushotham et al, 1995a), but we do not know the consequence to gland function during chronic activation as in neoplasms.…”

Section: (V) Summary/future Directionsmentioning

confidence: 99%

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The Role of Phosphotyrosine Signaling Pathway in Parotid Gland Proliferation and Function

Purushotham

Humphreys‐Beher

1995

Critical Reviews in Oral Biology & Medicine

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Tyrosine phosphorylation and the intracellular signaling processes associated with it have been the focus of intense study due to its importance in the regulation of biological processes as diverse as cell proliferation and cell differentiation. While much of what we now understand has been derived from the study of cell lines and tumor cells, the salivary glands provide a model to examine the effects of tyrosine kinases and tyrosine phosphatases in a normal differentiated tissue. This review will focus, therefore, on the role tyrosine kinases and phosphatases play in inducing the transition from stasis to active proliferation and their potential role in mediating secretory function of the salivary glands.

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Section: Mediation Of Secretionmentioning

confidence: 99%

Section: (V) Summary/future Directionsmentioning

confidence: 99%

The Role of Phosphotyrosine Signaling Pathway in Parotid Gland Proliferation and Function

Purushotham

Humphreys‐Beher

1995

Critical Reviews in Oral Biology & Medicine

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“…Salivary glands are influenced by exogenous circulating epidermal growth factor (EGF), which stimulates their development and causes changes in their activity (Purushotham et al. 1995; Ohlsson et al.…”

Section: Introductionmentioning

confidence: 99%

Subcellular localization of epidermal growth factor receptor in human submandibular gland

et al. 2006

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The subcellular distribution of the epidermal growth factor receptor (EGFr) was demonstrated in the normal human submandibular gland by means of immunogold cytochemistry. EGFr labelling appeared in both acinar and ductal cells, where strong immunoreactivity was associated with a tubulovesicular system near the basolateral surfaces. In addition, groups of reactive vesicles were highlighted among secretory granules of both serous and mucous cells and at the apex of ductal cells. Basolateral vesicles were interpreted as being a result of EGFr internalization after activation by an exogenous ligand, although the functional meaning of those located apically remains unclear.

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“…This closeness suggests that EGFR could interact directly with granules, perhaps regulating the secretory process in an autocrine fashion. Moreover, amylase secretion is known to be affected by EGFR activation (Purushotham et al, 1995). Mucin secretion also is enhanced by EGFR activation (Nadel, 2001), but this action is ascribed to effects of the signal transduction on DNA.…”

Section: Introductionmentioning

confidence: 98%

Localisation of Epidermal Growth Factor Receptor in Mucous Cells of Human Salivary Glands

Piludu¹,

Ms²,

Isola³

et al. 2003

European Journal of Morphology

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EGFR activation has been related to an increase in synthesis and secretion of mucins in epithelial cells, so that the use of EGFR tyrosine kinase inhibitors has been proposed in the therapy of mucin hypersecretory diseases. In this paper, we describe the ultrastructural localisation of EGFR in the mucous elements of human major and minor salivary glands and relate it to mucin distribution. A post-embedding immunogold staining method has been applied to normal surgical samples of human submandibular, sublingual, and labial glands, using a mouse monoclonal antibody specific for the intracellular domain of human EGFR. In mucous cells of all the glands examined, specific reactivity was detected in the cytoplasmic basolateral portions and near the mucous droplets, but not on cell surfaces. Since this pattern of labelling must be related to the internalisation process of the ligand-GFR complex, our results support the hypothesis that EGFR activation takes place in mucous cells and affects mucin production in human salivary glands.

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Effect of EGF on rat parotid gland secretory function

Cited by 6 publications

References 23 publications

The Role of Phosphotyrosine Signaling Pathway in Parotid Gland Proliferation and Function

The Role of Phosphotyrosine Signaling Pathway in Parotid Gland Proliferation and Function

Subcellular localization of epidermal growth factor receptor in human submandibular gland

Localisation of Epidermal Growth Factor Receptor in Mucous Cells of Human Salivary Glands

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