Effect of EGF and bFGF on Fibroblast Proliferation and Angiogenic Cytokine Production from Cultured Dermal Substitutes

Abstract

Growth factors accelerate wound healing but the underlying mechanisms remain poorly understood. The aim of this study was to investigate the effect of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) on fibroblast proliferation and production of angiogenic factors from cultured dermal substitutes (CDS). In the first experiment, fibroblasts were seeded into a flask at a density of 1 × 10(4) cells/cm(2).Cell proliferation was assessed after culturing in media containing EGF or bFGF at concentrations ranging from 2 to 50 μg. The number of fibroblasts increased significantly in the presence of EGF or bFGF, but fibroblasts detached from the flasks in the presence of 50 μg bFGF. In the second experiment, CDS were prepared by incorporating fibroblasts into collagen gels. To make a wound surface model, the CDS was elevated to the air-liquid interface, on which a spongy sheet of hyaluronic acid (HA) containing EGF or bFGF was placed. The amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) released from the CDS after 1 week of cultivation was measured by ELISA. When the CDS was covered with a HA sponge containing EGF (Group 1), fibroblasts released 3.5-times more VEGF compared with a HA-alone sponge (control group). When covered with a HA sponge containing bFGF (Group 2), 8.7-times more VEGF was released compared with the control group. Fibroblasts in Groups 1 and 2 released 9.6- and 9.3-times more HGF, respectively, compared with the control group. Thus, EGF stimulates fibroblasts to produce VEGF and HGF, in addition to its ability to enhance epidermal cell proliferation.

PLGA/gelatin hybrid nanofibrous scaffolds encapsulating EGF for skin regeneration

“…The effect of EGF on increased cell proliferation and mitosis has been reported. 9,14,39 Also, gelatin increased cell proliferation in the hybrid scaffolds as mentioned before. Therefore, the PLGA and hybrid scaffold were selected for the microscopic and histological analyses.…”

<i>In Vitro</i> Analysis of VEGF and HGF Production by Fibroblast in Cultured Dermal Substitute Combined with EGF-Incorporating Top Dressing

“…In a previous study [28], we reported that fibroblast proliferation was facilitated in a flask containing culture medium with bFGF ranging from 2 to 10 µg/15mL, but failed to facilitate proliferation at 50 µg/15mL. On the other hand, fibroblast proliferation was facilitated in a flask containing culture medium with EGF ranging from 2 to 50 µg/15mL.…”

Development of anti-adhesive spongy sheet composed of hyaluronic acid and collagen containing epidermal growth factor

“…[16] Briefly, fibroblasts (cell line derived from human dermis) were suspended in double concentrated Dulbecco's modified Eagle's medium supplemented with 20% fetal bovine serum at a density of 80 × 10 5 cells/12 mL. Fibroblast suspension (12 mL) was mixed with sterilized 1% Col solution (12 mL) at a temperature below 10°C.…”

“…[1][2][3][4][5][6][7][8][9][10][11][12][13] We have developed several types of wound dressing composed of hyaluronic acid (HA) and collagen (Col) spongy sheet containing epidermal growth factor (EGF). [14][15][16][17] Both HA and Col have the potential to facilitate wound healing. In the development of anti-adhesive products, researchers have focused on preventing surgically excised tissue from adhering to surrounding tissues using mechanical barriers such as membranes and spongy sheets.…”