Effect of efficient pectinolytic bacterial isolates on retting and fibre quality of jute

Biochemical characterization and Biolog based identification of efficient Jute retting bacterial isolates from retting water

“…The result clearly indicates that during the early stage of retting water complex Pectinolytic compound breaks down to simple form as PG acid through several enzymes like PLN which later breaks down by PG. Another thing which may conclude from this view ( Table 2) that in jute retting microorganisms PG activity and PLN activity both are independent of each other because we have not get any microbial strains having both of high PLN and high PG activity at 48 and 72 h. Similar results obtained by Das et al (2012a). where microbial strains having higher PG activity failed to show higher PLN activity.…”

“…This is because a jute retting microbe is supposed to be highly efficient which is having high Pectinolytic and Xylanase (EC 3.2.1.8) (Collins et al, 2005) activity with no or less Cellulase (EC 3.2.1.4) (Sarrouh et al, 2012) activity. During retting, degradation of pectin carried out by Pectinase mainly Polygalacturonase (PG) and pectin lyase (PLN) (EC 4.2.2.10) (Duvetter et al, 2009) activity of retting microbes while partial degradation of Xylan (major component of hemicellulose) done by Xylanase to make the fiber soft in nature (Ahmed and Akhter, 2001;Das et al, 2012a). Application of mixed enzyme (Cellulase, Xylanase and Pectinase) on jute shown significant positive changes on physical characteristics of jute fibers in terms of whiteness index, brightness index (Vigneswaran and Jayapriya, 2009).…”

“…Application of mixed enzyme (Cellulase, Xylanase and Pectinase) on jute shown significant positive changes on physical characteristics of jute fibers in terms of whiteness index, brightness index (Vigneswaran and Jayapriya, 2009). Until now there were several attempts that has been taken to test the pectinolytic activity of microorganisms like fungi (Haque et al,2001b), bacteria (Das et al, 2012a) for retting and pilling of jute (Banik and Ghosh, 2008) and identified through technique like 16S rDNA analysis (Das et al, 2012a;Das et al, 2011;Munshi and Chattoo, 2008) or through Bergey's manual (Banik and Ghosh, 2008;Ali, 1958;Jalaluddin, 1965) and assessment of bacterial community done through biolog ecoplates under different stages of jute retting at different location (Das et al, 2013). Until now there is no report available on the identification of retting microorganisms through using BIOLOG Microplate system from retting water.…”

Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting

“…Microbial consortium 1 (MC 1) consists of 4 pectinolytic bacteria of Bacillus sp.L6, Bacillus pumilus EK 17, B. pumilus Geo-03-422 and B. pumilus IK-MB12-518 F (Das et al, 2012), microbial consortium 2 (MC2) consists of three different strains of Bacillus pumilus and one strain of Bacillus subtilis (Srivastava et al, 2012) and microbial consortium 3 (MC 3) consists of Bacillus pumilus IMAU80221, B. pumilus GVC11 and B. pumilus SYBC-W (Majumdar et al, 2009;Das et al, 2015). The microbes of MC1 & MC2 have cellulolytic activities whereas; the microbes of MC3 do not have any cellulolytic activities.…”

“…secreted by the retting microbes. Uses of pectinolytic bacterial consortium for quick retting of jute have been reported by several researchers (Banik et al, 2007;Majumdar et al, 2009;Das et al, 2012;Das et al, 2015). The microbes of microbial consortium in useful association release pectinolytic enzymes (polygalacturonase, pectin lyase) and xylanase, which in turn degrade the pectin and xylan present as cementing materials.…”