Effect of EFA deficiency on lipid transport from liver

Abstract

Studies are reported of the effect of an essential fatty acid (EFA) deficiency on synthesis of triglycerides (TG) and phospholipids (PL) and secretion of these compounds by livers of male Sprague-Dawley rats. Animals were fed a semipurified diet containing corn oil or hydrogenated coconut oil (HCO) as the sole source of fat or no fat from weaning to 20 weeks of age. Liver function of the animals in each group was compared by an isolated liver perfusion technique with perfusates containing erythrocytes and linoleate, and in vivo experiments via tail vein injection of palmitate-3H. Perfusion experiments showed that an EFA deficiency reduced the ability of the liver to secrete TG and PL. Accumulation of TG in the liver and its diminished secretion into the blood of EFA deficient animals were demonstrated by in vivo experiments with palmitate-3H. The rate of conversion of linoleate to arachidonate and synthesis of PL was greater in livers of EFA deficient rats than in the control, corn oil fed animals. The results suggest a relationship of EFA metabolism to lipid transport.

Effects of crude rapeseed oil on lipid composition in Arctic charr Salvelinus alpinus

“…Earlier studies have reported that salmonids fed diets low or deficient in EFA tend to develop swollen and pale livers with high lipid content (Henderson & Tocher, 1987). Similar results have been seen in rats and are assumed to be the effect of impairment of lipoprotein metabolism (Fukuzawa et al ., 1971). No visual effects were distinguished in this study that would indicate an abnormal or dysfunctional liver.…”

Medaka as a model for human nonalcoholic steatohepatitis

“…Therefore, when PUFAs are deficient, fatty acid oxidation declines and livers accumulate fat (Fukazawa et al, 1971). In our medaka NASH model, expression of the mRNA encoding the lipogenic transcription factor SREBP-1c was induced, and expression levels of the fatty acid synthesis pathway enzymes ACC1 and FAS were increased.…”

Effect of calorie to protein ratio on the degree of fatness in broilers fed on practical diets1

“…Donaldson et al, (1958) reported that increasing the amount of dietary fat increased carcass fat content while decreasing its protein content. On the other hand, Edwards and Hart (1971) did not observe any change in total carcass composition when all the nonprotein energy was derived from various oils and Fukazawa et al (1971) reported on fat accumulation in livers of rats fed on a diet deficient in essential fatty acids. Leveille and Sauberlich (1961) and Keyser et al (1968) observed a close relationship between dietary and serum protein concentrations.…”