Effect of edible oils on quercetin, kaempferol and galangin transport and conjugation in the intestinal Caco-2/HT29-MTX co-culture model

Flavonoid interactions during digestion, absorption, distribution and metabolism: a sequential structure–activity/property relationship-based approach in the study of bioavailability and bioactivity

“…Therefore, it can be said that flavonoids interact with fats by means of hydrophobic interactions, which are rather weak. Using a Caco-2/HT29-MTX co-culture model, Jailani & Williamson (2014) found that co-administration of flavonoids with commonly used dietary oils altered the absorption behavior of flavonoids depending on their hydrophobicity. For instance, addition of oil significantly increased the production of quercetin and kaempferol conjugates (especially sulfates) at the basolateral compartment by up to three and four times, respectively.…”

Review on the Use of Cell Cultures to Study Metabolism, Transport, and Accumulation of Flavonoids: From Mono‐Cultures to Co‐Culture Systems

“…Recently, a Caco‐2/HT29‐MTX co‐culture system was used to investigate the effect of edible oils on quercetin, kaempferol, and galangin transport. Results suggested that aglycones and conjugates were transported to the basolateral compartment of the co‐culture and that metabolism was enhanced with the addition of oils, except for galangin (Jailani and Williamson ). It must be noted, however, that HT29‐MTX cells mainly produce gastric‐type mucins, MUC5AC, which are different from those that are produced in the intestines, MUC2 and MUC3 (Lesuffleur and others ; Hennebicq‐Reig and others ).…”

Assessing the bioavailability of polyphenols and antioxidant properties of extra virgin argan oil by simulated digestion and Caco-2 cell assays. Comparative study with extra virgin olive oil

“…Studies have also shown that significant amounts of bioactive compounds may remain in the residues after in vitro digestion (Pastoriza et al, 2011), usually ignored. In recent decades, in vitro methods have been improved by incorporating enterocyte-like cell cultures, such as the Caco-2 cell line (Jailani & Williamson, 2014;Mesías, Seiquer, & Navarro, 2009). In culture, this cell line exhibits many properties of the normal intestinal epithelium, and it has been used as a suitable model to study the absorption of phenolics (Soler et al, 2010) and antioxidant cellular response (Ruiz-Roca, Delgado-Andrade, Navarro, & Seiquer, 2011).…”

“…In vitro methods are a good alternative and generally consist of a simulation of gastrointestinal digestion prior to analyte determination. This method has been widely used to evaluate mineral bioaccessibility (Cabrera-Vique & Bouzas, 2009;Seiquer, Delgado-Andrade, Haro, & Navarro, 2010) and bioactive compounds such as polyphenols (Jailani & Williamson, 2014), and also in testing the antioxidant properties of different http://dx.doi.org/10.1016/j.foodchem.2015.05.006 0308-8146/Ó 2015 Elsevier Ltd. All rights reserved. foods after the simulated digestion process (Chen et al, 2014;Dinnela, Minichino, D'Andrea, & Monteleone, 2007;Pastoriza, Delgado-Andrade, Haro, & Rufián-Henares, 2011).…”