Effect of eccentric contraction on satellite cell activation in human vastus lateralis muscle

Imaoka, Yoko; Kawai, Minako; Mori, Futoshi; Miyata, Hirofumi

doi:10.1007/s12576-015-0385-4

Cited by 12 publications

(9 citation statements)

References 42 publications

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“…In fact, previous studies have shown a greater muscle damage in VI when compared to other vastii muscles after eccentric exercise using magnetic resonance imaging in human subjects . It should be noted that many of the studies in which histological changes induced by eccentric knee extensor exercise were investigated, muscle biopsy samples were obtained from VL only . It is possible that histological changes observed in VL underestimated global muscle damage.…”

Section: Perspectivementioning

confidence: 98%

Vastus intermedius vs vastus lateralis fascicle behaviors during maximal concentric and eccentric contractions

Ando

Nosaka

Tomita

et al. 2017

Scandinavian Med Sci Sports

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Vastus intermedius (VI) plays a major role in knee extension, but its fascicle behaviors during dynamic contractions are not well understood. This study aimed to compare VI and vastus lateralis (VL) fascicle behaviors during singular maximal concentric and eccentric contractions. Thirteen men (27.1 ± 3.4 years) performed maximal isokinetic concentric contractions through knee joint angles of 105° to 35° (0° = full extension) and eccentric contractions from 35° to 105° at an angular velocity of 30°/s. Longitudinal VI and VL sonographic images were simultaneously recorded at 30 Hz, and muscle fascicle lengths at the knee joint angles of 40° and 100° were measured to compare the magnitudes of fascicle length change between the muscles. During concentric contractions, VI and VL fascicle lengths at 100° were 108 ± 12 mm and 104 ± 12 mm, respectively, and shortened by 36 ± 12 mm for VI and 28 ± 13 mm for VL (not statistically different; P = .13) at 40°. During eccentric contractions, VI and VL fascicle lengths at 40° were 72 ± 7 mm and 75 ± 8 mm, respectively, but lengthened by 35 ± 9 mm for VI and 24 ± 5 mm for VL at 100°, with a significant difference between the muscles (P = .01). These results indicate that VI fascicles are lengthened 1.4 times more than VL fascicles during eccentric contractions, whereas VI and VL fascicles shorten similarly during concentric contractions. This suggests a possibility that a greater mechanical strain is imposed to VI than VL during eccentric contractions.

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Section: Perspectivementioning

confidence: 98%

Vastus intermedius vs vastus lateralis fascicle behaviors during maximal concentric and eccentric contractions

Ando

Nosaka

Tomita

et al. 2017

Scandinavian Med Sci Sports

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“…The number of satellite cells in disused muscle is known to be reduced, caused by impaired proliferation (Zammit et al 2006;Li et al 2016). The mechanical stretch causes satellite cell activation (Imaoka et al 2015). In this study, the satellite cells from disused muscle retained both their original proportions and myogenic potential as much as that from the intact muscle.…”

Section: Discussionmentioning

confidence: 53%

Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration

Koide

Hagiwara

Tsuchiya

et al. 2018

Tohoku J. Exp. Med.

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Rotator cuff tears (RCTs) are a common shoulder problem in the elderly that can lead to both muscle atrophy and fatty infiltration due to less physical load. Satellite cells, quiescent cells under the basal lamina of skeletal muscle fibers, play a major role in muscle regeneration. However, the myogenic potency of human satellite cells in muscles with fatty infiltration is unclear due to the difficulty in isolating from small samples, and the mechanism of the progression of fatty infiltration has not been elucidated. The purpose of this study was to analyze the population of myogenic and adipogenic cells in disused supraspinatus (SSP) and intact subscapularis (SSC) muscles of the RCTs from the same patients using fluorescenceactivated cell sorting. The microstructure of the muscle with fatty infiltration was observed as a whole mount condition under multi-photon microscopy. Myogenic differentiation potential and gene expression were evaluated in satellite cells. The results showed that the SSP muscle with greater fatty infiltration surrounded by collagen fibers compared with the SSC muscle under multi-photon microscopy. A positive correlation was observed between the ratio of muscle volume to fat volume and the ratio of myogenic precursor to adipogenic precursor. Although no difference was observed in the myogenic potential between the two groups in cell culture, satellite cells in the disused SSP muscle showed higher intrinsic myogenic gene expression than those in the intact SSC muscle. Our results indicate that satellite cells from the disused SSP retain sufficient potential of muscle growth despite the fatty infiltration.

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“…As stated above, TRPC1 likely plays an important role in satellite cell activation. Consistent with this, TRPC1 expression was significantly increased in satellite cells of the left VL muscle, suggesting that eccentric but not concentric exercise activates satellite cells in a TRPC1-dependent manner [21].…”

Section: Trpc1mentioning

confidence: 63%

“…As a signal mediator, Ca 2+ also plays a critical role in the activation of Nox proteins. Increased activity of TRPC channels has been reported in skeletal muscle after physical activity [21, 66]. In skeletal muscle, acute exercise increases Nox2 protein activity.…”

Section: Perspectivementioning

confidence: 99%

TRPC channels in exercise-mimetic therapy

Numaga‐Tomita

Oda

Nishiyama

et al. 2018

Pflugers Arch - Eur J Physiol

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Physical exercise yields beneficial effects on all types of muscle cells, which are essential for the maintenance of cardiovascular homeostasis and good blood circulation. Daily moderate exercise increases systemic antioxidative capacity, which can lead to the prevention of the onset and progression of oxidative stress-related diseases. Therefore, exercise is now widely accepted as one of the best therapeutic strategies for the treatment of ischemic (hypoxic) diseases. Canonical transient receptor potential (TRPC) proteins are non-selective cation channels activated by mechanical stress and/or stimulation of phospholipase C-coupled surface receptors. TRPC channels, especially diacylglycerol-activated TRPC channels (TRPC3 and TRPC6; TRPC3/6), play a key role in the development of cardiovascular remodeling. We have recently found that physical interaction between TRPC3 and NADPH oxidase (Nox) 2 under hypoxic stress promotes Nox2-dependent reactive oxygen species (ROS) production and mediates rodent cardiac plasticity, and inhibition of the TRPC3-Nox2 protein complex results in enhancement of myocardial compliance and flexibility similar to that observed in exercise-treated hearts. In this review, we describe current understanding of the roles of TRPC channels in striated muscle (patho)physiology and propose that targeting TRPC-based protein complexes could be a new strategy to imitate exercise therapy.

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Effect of eccentric contraction on satellite cell activation in human vastus lateralis muscle

Cited by 12 publications

References 42 publications

Vastus intermedius vs vastus lateralis fascicle behaviors during maximal concentric and eccentric contractions

Vastus intermedius vs vastus lateralis fascicle behaviors during maximal concentric and eccentric contractions

Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration

TRPC channels in exercise-mimetic therapy

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