Effect of Early Antibiotic Treatment on the Antibody Response to Cytoplasmic Proteins of
            <i>Brucella melitensis</i>
            in Mice

Bowden, Robert A.; Racaro, Graciela C.; Baldi, Pablo C.

doi:10.1128/cdli.6.3.440-443.1999

Cited by 11 publications

(8 citation statements)

References 14 publications

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“…The most commonly used tests are the Serum Agglutination Test (SAT), the Coombs anti-Brucella test, the Rose Bengal Test (RBT) and complement fixation. During the last decade, radioimmunoassay [7,8] and enzyme immunoassay [9][10] tests have also been used. These present technical difficulties since they require skilled personnel and high-cost material.…”

Section: Introductionmentioning

confidence: 99%

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Salmani¹,

Siadat²,

Fallahian³

et al. 2009

American J. of Infectious Diseases

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Problem statement: Brucellosis is a globally found infectious disease and there is no licensed vaccine against human brucellosis. The present study carried-out to evaluate the potency of our modified extracted lipopolysaccharide (LPS) of B. abortus to elicit specific anti-Brucella antibodies in animal model (Rabbit) as a part of a candidate vaccine for brucellosis. Lipopolysaccharide is one of the main virulence factors and the most immunogenic structure of smooth strains of Brucella. Approach: Lipopolysaccharide of B. abortus S99 (S-LPS) initially extracted through an optimized method as described previously. After biochemical and pyrogenicity evaluations of the extracted S-LPS humoral immune response against the extracted LPS analyzed in animal model through serological assays such as Rose Bengal assay, Rapid agglutination (Rapid Wright) test and Standard agglutination test (SAT or Wright test) to demonstrate the specific elicited antibodies against the injected LPS. In addition, the interaction of LPS and anti-LPS antibodies was demonstrated by Agarose Gel Immunodiffusion (AGID) assay. Results: Higher doses of B. abortus S99 LPS caused less or equal body temperature increase in comparison to E. coli LPS doses. Sera of immunized animals had been reported positive by RBT because of B. abortus LPS immunogenicity which we extracted through our optimized method. The highest titer of anti-Brucella antibodies detected two weeks after the third immunization (assayed by rapid slide agglutination and standard agglutination tests). Anti-Brucella antibodies of immunized animals reacted more specifically with the LPS of B. abortus in comparison with E. coli LPS and precipitation lines between B. abortus LPS and immune sera appeared after 30 min while detected after three hours for E. coli LPS. Conclusions/Recommendations: The properties of B. abortus S99 LPS concluded from the present study results, suggest the possible use of this component as a carrier or a part of a sub-unit or conjugated vaccine for human brucellosis.

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Section: Introductionmentioning

confidence: 99%

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Salmani¹,

Siadat²,

Fallahian³

et al. 2009

American J. of Infectious Diseases

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“…The retention of the implant and degree of debridement used in this study may have allowed the establishment of a biofilm with subpopulations of bacteria that were unaffected by the specific antibiotic used, and which were able to proliferate with increased time from debridement and completion of antibiotic therapy. In addition, besides killing or inhibiting the growth of microorganisms, antibiotics may blunt the host immune response to infection, similar to the way it impairs antibody response to some infections [2,20,33]. For example, there is evidence that antibiotics may affect the metabolism of bacteria [12], and may interfere with phagocyte function [10,14] and cytokine production [l].…”

mentioning

confidence: 99%

Characterization of a chronic infection in an internally‐stabilized segmental defect in the rat femur

Chen

Tsukayama

Kidder

et al. 2005

Journal Orthopaedic Research

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The aim of this study was to characterize a new model of chronic osteomyelitis with clinically relevant features. A segmental defect of critical size was surgically created in the rat femur, stabilized with a polyacetyl plate and Kirschner wires, and contaminated with bacteria. The animals were allowed to recover while the contamination progressed to a chronic infection. At a later point in time, the defect was surgically debrided without removing the implant. Further treatments of interest, such as antibiotic therapy or application of an osteogenic agent, could be introduced at this time. To implement this model, an initial experiment was performed to determine the bacterial inoculum and time from contamination that would reliably result in an infected defect without causing excessive bone damage by the time debridement surgery was performed. The number of recovered bacteria, degree of radiographic bony lysis, and torsional stiffness of the defect fixation were measured in 192 rats as a function of 4 inocula of Staphylococcus aureus (lo', lo4, 10' or lo6 CFUs) and 4 times from contamination (1,2, 3 or 4 weeks). A lo4 CFU inoculum over 2 weeks was found to consistently create an infection without severe lysis and loss of fixation stability. Based on these values, a second experiment was performed in 96 rats to characterize the debrided defect over time (2,4,8 and 12 weeks after debridement), with and without 4 weeks of the antibiotic ceftriaxone, in terms of the same outcome variables. Infection was persistent in all animals in spite of debridement and antibiotic therapy. Antibiotic therapy did not reduce the degree of bony lysis. Compared with animals not given antibiotic, bacterial counts significantly decreased during the period of antibiotic therapy, but then rebounded to significantly higher levels at 12 weeks. This model allows us to perform further studies on differing regimens of antibiotic therapy and their relationship to surgical dtbridement, and on the efficacy of osteogenic agents in the presence of infection.

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“…They identified in mice that commencement of doxycycline at seven days post vaccination (for Brucella melitensis) resulted in complete reduction of IgG specific www.nature.com/scientificreports www.nature.com/scientificreports/ antibodies for greater than 50 days. Conversely, if antibiotics were commenced 28 days post vaccination, IgG specific antibodies were observed to remain, although at lower levels, compared to no antibiotic treatment 32 . In the current koala trial, all animals had pre-existing Chlamydia infections that had persisted long enough to cause signs of cystitis.…”

Section: Discussionmentioning

confidence: 88%

“…The lasting effect that antibiotics have on the humoral immune response has been demonstrated to depend on the antibiotic used, the dose schedule and the antigen used to vaccinate 30 . Bowden et al (1998) identified that the time between vaccination and commencement of antibiotics can significantly affect the development of antigen specific IgG antibodies 32 . They identified in mice that commencement of doxycycline at seven days post vaccination (for Brucella melitensis) resulted in complete reduction of IgG specific www.nature.com/scientificreports www.nature.com/scientificreports/ antibodies for greater than 50 days.…”

Section: Discussionmentioning

confidence: 99%

Vaccination of koalas during antibiotic treatment for Chlamydia-induced cystitis induces an improved antibody response to Chlamydia pecorum

Phillips

Chaban

Olagoke

et al. 2020

Sci Rep

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Chlamydia infection and disease are endemic in free-ranging koalas. Antibiotics remain the front line treatment for Chlamydia in koalas, despite their rates of treatment failure and adverse gut dysbiosis outcomes. A Chlamydia vaccine for koalas has shown promise for replacing antibiotic treatment in mild ocular Chlamydia disease. in more severe disease presentations that require antibiotic intervention, the effect of vaccinating during antibiotic use is not currently known. This study investigated whether a productive immune response could be induced by vaccinating koalas during antibiotic treatment for Chlamydia-induced cystitis. plasma igG antibody levels against the C. pecorum major outer membrane protein (MoMp) dropped during antibiotic treatment in both vaccinated and unvaccinated koalas. post-treatment, igG levels recovered. the igG antibodies from naturally-infected, vaccinated koalas recognised a greater proportion of the MoMp protein compared to their naturally-infected, unvaccinated counterparts. furthermore, peripheral blood mononuclear cell gene expression revealed an up-regulation in genes related to neutrophil degranulation in vaccinated koalas during the first month post-vaccination. These findings show that vaccination of koalas while they are being treated with antibiotics for cystitis can result in the generation of a productive immune response, in the form of increased and expanded igG production and host response through neutrophil degranulation.

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Effect of Early Antibiotic Treatment on the Antibody Response to Cytoplasmic Proteins of Brucella melitensis in Mice

Cited by 11 publications

References 14 publications

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Characterization of a chronic infection in an internally‐stabilized segmental defect in the rat femur

Vaccination of koalas during antibiotic treatment for Chlamydia-induced cystitis induces an improved antibody response to Chlamydia pecorum

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