Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

García, Elina V.; Miceli, Dora C.; Rizo, Gabriela; Valdecantos, Pablo A.; Barrera, Daniel

doi:10.1016/j.theriogenology.2015.04.018

Cited by 20 publications

(22 citation statements)

References 51 publications

(67 reference statements)

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“…Further specific to up‐regulated genes, the Q‐PCR analysis again showed that the expression of BMP5, FGFR2 and CD9 genes was significantly up‐regulated in the BCL2 group vs. the NC group (Figure 3D). Previous studies demonstrated that these three genes play a role in the maintenance of pluripotency and survival 32‐35 . The increased expression of FGFR2, which is one of the receptors of FGF2, can significantly amplify the effect of FGF2.…”

Section: Resultsmentioning

confidence: 99%

BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2

et al. 2020

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Objectives The establishment of porcine pluripotent stem cells (pPSCs) is still a critical topic. However, all pPSCs were failed to contribute to efficient chimeric pig and were extremely sensitive to changes of culture conditions. This study aimed to investigate the role of BCL2 in pPSCs and further explain the mechanism. Materials and Methods Porcine BCL2 gene was cloned and overexpressed in porcine induce pluripotent stem cells (piPSCs). Digital RNA‐seq was performed to explain the mechanism of anti‐apoptosis. Finally, the cells carrying BCL2 were injected into mouse early embryo to evaluate its chimeric ability. Results Here, we found that overexpression of porcine BCL2 gene significantly improved the survivability of piPSCs and the efficiency of embryonic chimerism, and did not wreck the pluripotency of piPSCs. Furthermore, the Digital RNA‐seq analysis revealed that BCL2, as a downstream gene of the PI3K signal pathway, enhanced the expression of PI3K signal pathway receptors, such as FGFR2, and further promoted oxidoreductases activity and lipid metabolism, thus maintaining the survival and pluripotency of piPSCs. Conclusion Our data not only suggested that porcine BCL2 gene could enhance the survivability and chimeric ability of pPSCs, but also explained the positive feedback mechanism in this process, providing strong support for the chimeric experiment of pPSCs.

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Section: Resultsmentioning

confidence: 99%

BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2

et al. 2020

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“…In reference to BMP receptors, previous studies demonstrated the expression of BMPR1B and BMPR2 in the bovine embryo, showing high abundance of their transcripts at the early embryonic stages (from 2-cell stage to 8-cell stage) (Garcia et al 2015). This temporal gene expression pattern of the receptors suggests that the embryo could respond to BMP ligands during the first developmental stages during transit through the oviduct.…”

Section: Figurementioning

confidence: 84%

“…It is well known that both BMP receptors, BMPR1B and BMPR2, play a critical role in preimplantation development and female fertility (Soyun et al 2001, Nagashima et al 2013, Graham et al 2014, de Mochel et al 2015. In addition, their transcript levels in the embryo are specifically elevated at early stages before EGA (Lee et al 2014, Garcia et al 2015). In the case of ID2, it is a target gene of BMP signaling and it acts as a transcription factor required for maintenance and differentiation of trophoblast cells (Janatpour et al 2000, Roberts et al 2004, Schiffmacher & Keefer 2013.…”

Section: Figurementioning

confidence: 99%

“…The presence of this factor in the oviductal fluid suggests that BMP ligands produced by the oviduct could have a paracrine action on the bovine embryo (Garcia et al 2014). In fact, the addition of BMP5 to the culture medium during the first two days after fertilization produces an increase in the blastocyst rate and the relative mRNA abundance of pluripotency-related genes (Garcia et al 2015). Interestingly, mRNA expression of BMP receptors is greater in early stage embryos (from 2-cell to 8-cell stage), suggesting that the embryo could be target of BMPs during its transit through the oviduct (Garcia et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the addition of BMP5 to the culture medium during the first two days after fertilization produces an increase in the blastocyst rate and the relative mRNA abundance of pluripotency-related genes (Garcia et al 2015). Interestingly, mRNA expression of BMP receptors is greater in early stage embryos (from 2-cell to 8-cell stage), suggesting that the embryo could be target of BMPs during its transit through the oviduct (Garcia et al 2015). Moreover, in a recent study by RNA sequencing, Maillo and coworkers (Maillo et al 2015) observed that the presence of multiple embryos induces changes in the expression levels of several genes in the bovine oviduct in vivo, included among them are various genes associated with the BMP signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

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Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling

et al. 2017

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Signaling components of bone morphogenetic proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA). Exposure to embryos, irrespective of the period, significantly reduced the relative abundance of, ,, and mRNAs in BOEC. In contrast, embryos that interacted with BOEC before EGA showed a significant increase in the relative abundance of mRNA at the 8-cell stage compared to embryos cultured without BOEC. Moreover, embryos at the 16-cell stage that interacted with BOEC during EGA showed a significant increase in, and mRNA. These results demonstrate that embryo-oviduct interaction induces specific changes in the transcriptional levels of BMP signaling, causing a bidirectional response that reduces the expression levels of this signaling in the oviductal cells while increases them in the early embryo. This suggests that BMP signaling pathway could be involved in an early cross talk between the bovine embryo and the oviduct during the first stages of development.

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Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus‐oocyte complexes: A biological and spectroscopic approach

Rizo

Barrera

Jimenez

et al. 2020

Molecular Reproduction Devel

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This study deals with the effect of plasminogen/plasmin on the in vitro maturation (IVM) of bovine cumulus‐oocyte complexes (COCs). Exogenous plasminogen activator streptokinase (SK) added to the IVM medium revealed similar values of cumulus expansion and oocyte nuclear maturation compared to controls (standard IVM medium). However, a decrease in both determinations was observed in COCs matured with the supplementation of ɛ‐aminocaproic acid (ɛ‐ACA), a specific plasmin inhibitor. After in vitro fertilization, no differences were observed in either cleavage or blastocyst rates between SK and control groups; however, ε‐ACA treatment caused a decrease in both developmental rates. Zona pellucida (ZP) digestion time decreased in the SK group while it increased in the ε‐ACA group. Raman microspectroscopy revealed an increase in the intensity of the band corresponding to the glycerol group of sialic acid in the ZP of oocytes matured with SK, whereas ZP spectra of oocytes treated with ɛ‐ACA presented similarities with immature oocytes. The results indicate that although treatment with SK did not alter oocyte developmental competence, it induced modifications in the ZP of oocytes that could modify the folding of glycoproteins. Plasmin inhibition impairs oocyte maturation and has an impact on embryo development, thus evidencing the importance of this protease during IVM.

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Effect of early addition of bone morphogenetic protein 5 (BMP5) to embryo culture medium on in vitro development and expression of developmentally important genes in bovine preimplantation embryos

Cited by 20 publications

References 51 publications

BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2

BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2

Bovine embryo-oviduct interaction in vitro reveals an early cross talk mediated by BMP signaling

Exogenous activation and inhibition of plasminogen/plasmin activity during in vitro maturation of bovine cumulus‐oocyte complexes: A biological and spectroscopic approach

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