This study quantified in vitro the root dentin moisture when 10% formalin (Group A), 3% sodium azide (Group B), and distilled water (Group C) were used as teeth storage media. The root dentin moisture of 66 extracted human mandibular single-rooted teeth was measured at baseline (day 0) and at 1, 3, 7, and 14 days using a digital grain moisture meter. The baseline dentin moisture value was used as a covariate in the generalized estimating equation (GEE) analysis. The mean dentin moisture values (%) ± standard deviation on days 0, 1, 3, 7, and 14 were 10.6±0.64, 14.3±0.71, 14.6±0.84, 14.4±0.64, and 14.7±0.75 (Group A); 11.4±0.94, 14.6±0.95, 14.6±0.76, 14.6±0.93, and 14.8±0.81 (Group B); and 10.2±0.95, 12.8±0.90, 13.3±0.95, 13.0±0.91, and 13.2±0.89 (Group C), respectively. The dentin moisture increased in all three groups; however, there was no overall significant difference in moisture between the formalin and sodium azide groups.