Effect of drying on the viability of the probiotic bacteriumLactobacillus plantarumLab9 (CPQBA 144-09 DRM 03) impregnated in the feed for tilapia (Oreochromissp.)

Abstract

Drying experiments were conducted at different temperatures and air flux velocities to determine the proper drying conditions for reducing moisture in commercial fish feed impregnated with probiotic lactic acid bacteria and to assess the effect on bacteria viability over time. At temperatures of 45°C, the drying time was shorter, without the air flux velocity under study having a relevant influence. The drying conditions influenced the viability of the bacteria in the feed; the least loss of viability was obtained with a velocity of 0.8 m s À1 and a temperature of 45°C during 15 min. Using these drying conditions, 5 kg of feed was dried and stored for a month at temperature of 26°C and relative humidity of 75%. The viability of the bacteria and the moisture of the feed were measured every 3 days during the storage period. Loss of viability followed first order kinetics, with a constant k of 0.112 days À1 . Thus, the viability of the bacteria in the feed is less than 10 6 CFU g À1 after 43 days.

"Evaluation of Bacterial Isolates from Seaweed Sargassum oligocystum Montagne as Probiotic Supplements in Commercial Fish Feeds"

“…Feeds.The duration of viability of Bacillus cereus complex isolates in commercial fish feed was determined for 24 days at a 3-day interval with a population count between 10 6 CFU/ml to 10 9 CFU/ml per sampling as the accepted viable count. This was supported by the studies conducted on halibut, H. hippoglosus (Ottensen & Olafsen, 2000), crab, P. pelagicus larvae and Lactobacillus based probiotics in teleost models (Uma et al, 1999;Dash et al, 2014;Londoño, Toro & Bolivar, 2015).…”

“…It was further added with 100 ml water per 100 g mixture to uniformly distribute the mixture. The 100 g bacterial culture was mixed thoroughly on feeds at two different formulations (Londoño, Toro, & Bolivar, 2015).…”

Current Applications and Future Trends of Dehydrated Lactic Acid Bacteria for Incorporation in Animal Feed Products

“…Furthermore, air heat-drying (AHD), employing various methods such as convective drying or traditional oven drying, has been employed as a simple and cost-effective approach to incorporate dried probiotic microorganisms directly into animal feed [58,63,92,93].…”