Effect of drought and rewatering on the cellular status and antioxidant response of<i>Medicago truncatula</i>plants

Filippou, Panagiota; Antoniou, Chrystalla; Fotopoulos, Vasileios

doi:10.4161/psb.6.2.14633

Cited by 109 publications

(69 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6E). Finally, we checked the expression of some antioxidant genes that are known to be altered in M. truncatula in response to drought stress (Filippou et al, 2011). We found that expression of the drought stressresponsive genes ALTERNATIVE OXIDASE (AOX; Medtr5g026620) and GLUTATHIONE S-TRANSFER-ASE (GST; Medtr1g026140) was also altered in latd Figure 4.…”

Section: Decreasing Ros Levels Can Increase Cell Elongation In Latd Rmentioning

confidence: 99%

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

2014

View full text Add to dashboard Cite

Abscisic acid (ABA) modulates root growth in plants grown under normal and stress conditions and can rescue the root growth defects of the Medicago truncatula lateral root-organ defective (latd) mutant. Here, we demonstrate that reactive oxygen species (ROS) function downstream of ABA in the regulation of root growth by controlling cell elongation. We also show that the MtLATD/NUMEROUS INFECTIONS AND POLYPHENOLICS (NIP) nitrate transporter is required for ROS homeostasis and cell elongation in roots and that this balance is perturbed in latd mutants, leading to an excess of superoxide and hydrogen peroxide and a corresponding decrease in cell elongation. We found that expression of the superoxide-generating NADPH oxidase genes, MtRbohA and MtRbohC (for respiratory burst oxidase homologs), is increased in latd roots and that inhibition of NADPH oxidase activity pharmacologically can both reduce latd root ROS levels and increase cell length, implicating NADPH oxidase function in latd root growth defects. Finally, we demonstrate that ABA treatment alleviates ectopic ROS accumulation in latd roots, restores MtRbohC expression to wild-type levels, and promotes an increase in cell length. Reducing the expression of MtRbohC using RNA interference leads to increased root elongation in both wild-type and latd roots. These results reveal a mechanism by which the MtLATD/NIP nitrate transporter and ABA modulate root elongation via superoxide generation by the MtRbohC NADPH oxidase.

show abstract

Section: Decreasing Ros Levels Can Increase Cell Elongation In Latd Rmentioning

confidence: 99%

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

2014

View full text Add to dashboard Cite

show abstract

“…An overview of changes in CAT activity at different stages indicates that levels of CAT decrease in advanced stages of plant growth; this could mean a decrease in CAT ability to scavenge ROS. Filippou et al (2011) also stated that the implementation of drought stress for 14 days induced CAT activity, compared to control. PPO activity also increased with increasing drought stress severity, but changes were different at various growth stages. )…”

Section: Discussionmentioning

confidence: 99%

Changes in enzymatic and nonenzymatic antioxidant defense mechanisms of canola seedlings at different drought stress and nitrogen levels

Ahmadi¹,

Ebadi²,

Jahanbakhsh³

et al. 2015

Turk J Agric For

View full text Add to dashboard Cite

To evaluate the responses of canola seedlings to different levels of drought stress and nitrogen at different growth stages, a factorial experiment was conducted in a completely randomized design with three replications at the experimental greenhouse of Mohaghegh Ardabili University in 2013. Treatments included three levels of drought stress (30%, 50%, and 70% of FC) and five levels of nitrogen (control, based on soil test results; 25% less than the recommended level; 50% less than the recommended level; 25% more than the recommended level; and 50% more than the recommended level). The recommended level of nitrogen, based on soil test results, was 0.09 g of nitrogen per kilogram of soil. Results showed that drought stress, nitrogen, and their interaction significantly affected the enzymatic activity of antioxidant catalase (CAT), polyphenol oxidase (PPO), and peroxidase (PO) at various stages of growth. Proline was also affected by drought stress and nitrogen. Maximum CAT activity (794.04 OD mg protein min -1 ) was observed at the 4-6 leaf stage under mild drought stress conditions (50% of FC) and a nitrogen application rate 50% less than the recommended level. Increased rates of nitrogen enhanced the PPO activity at the 4-6 and 6-8 leaf stages. PO had a negative response to increased rates of nitrogen application. The highest rate of increase in proline was at the 8-10 leaf stage: 205% and 207% higher under mild drought stress (50% of FC) and severe drought stress (30% of FC) conditions, respectively, compared to favorable moisture conditions (70% of FC). Increased nitrogen application led to an increase in proline production at all stages of sampling (4-6, 6-8, and 8-10 leaf stages).

show abstract

“…Identification of drought responsive genes and validation of their association with drought tolerance are pre-requisites to establish if they can be employed in crop improvement programmes. Even though reports on gene response to drought stress and subsequent re-watering are available in other plants (Filippou et al 2011), no such studies were reported in Hevea. Hence this study was conducted in Hevea with an aim to assess the molecular response to drought stress and re-watering with reference to a specific drought responsive gene viz NAC tf.…”

Section: Introductionmentioning

confidence: 95%

Expression of NAC transcription factor is altered under intermittent drought stress and re-watered conditions in Hevea brasiliensis

et al. 2017

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Drought stress is one of the important factors that restrict the expansion of Hevea brasiliensis cultivation to nontraditional regions experiencing extreme weather conditions. Plants respond to drought stress by triggering expression of several drought responsive genes including transcription factors which in turn trigger expression of various downstream signalling pathways and adaptive networks. Expression of such drought responsive genes may revert back to their original level upon re-watering. However, no reports are available on such phenomenon in Hevea and hence, this study was initiated. For this purpose, NAC transcription factor (NAC tf) was chosen as candidate gene. Its expression levels were monitored under intermittent drought as well as irrigated conditions in two clones (RRII 105 and RRIM 600) of H. brasiliensis with contrasting tolerance level. Copy number of NAC tf was found similar in both the clones. Expression of NAC tf was found highly up-regulated in RRIM 600 (a relatively drought tolerant clone) than in RRII 105 (a relatively drought susceptible clone) throughout the drought incidences which upon re-watering, reached back to its original levels in both the clones. The study indicated the existence of an association between expression of NAC tf and drought tolerance trait exhibited by the tolerant clone RRIM 600. The study also proves the influence of drought and re-watering on the leaf photosynthesis and expression of NAC tf in H. brasiliensis.

show abstract

Effect of drought and rewatering on the cellular status and antioxidant response ofMedicago truncatulaplants

Cited by 109 publications

References 64 publications

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

Abscisic Acid and LATERAL ROOT ORGAN DEFECTIVE/NUMEROUS INFECTIONS AND POLYPHENOLICS Modulate Root Elongation via Reactive Oxygen Species in Medicago truncatula

Changes in enzymatic and nonenzymatic antioxidant defense mechanisms of canola seedlings at different drought stress and nitrogen levels

Expression of NAC transcription factor is altered under intermittent drought stress and re-watered conditions in Hevea brasiliensis

Contact Info

Product

Resources

About