Effect of Doxorubicin on Telomerase Activity and Apoptotic Gene Expression in Doxorubicin-Resistant and -Sensitive MCF-7 Cells

Eskiocak, Uğur; İşeri, Özlem Darcansoy; Kars, Meltem Demirel; Biçer, Atilla; Gündüz, Ufuk

doi:10.1159/000140464

Cited by 19 publications

(20 citation statements)

References 41 publications

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“…Telomerase is an attractive target for a mechanism-based therapeutic approach since its activation has been associated with sensitivity to chemotherapeutic agents in most types of cancer cells (12)(13)(14)(15)(16). However, little is known regarding the effect of biological response modifiers and chemotherapeutic agents on telomerase activity.…”

Section: Discussionmentioning

confidence: 99%

“…While the primary function of telomerase is the maintenance of structural integrity at the linear chromosome ends, recent studies have shown an association between telomerase activity and increased chemotherapeutic resistance consistent with poor prognosis in various types of cancer (12)(13)(14). Telomerase appears to mediate its protective effect by conferring resistance to apoptosis (15,16).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of telomerase activity in renal cell carcinoma cells by doxorubicin and anti-Fas monoclonal antibody

Jin¹

2009

Mol Med Rep

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Abstract. Telomerase, the ribonucleoprotein enzyme that maintains the telomeres of eukaryotic chromosomes, is an attractive target for a mechanism-based therapeutic approach as its activation has been associated with unlimited proliferation in most types of cancer cells. Here, we investigated the effect of chemotherapeutic or immunotherapeutic agents and anti-Fas monoclonal antibody (mab) on telomerase activity in renal cell carcinoma (rcc) cells. Primary rcc cells were surgically obtained from 24 patients with rcc. Telomerase activity and cytotoxicity were determined by the telomeric repeat amplification protocol and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, respectively. Telomerase activity was positive in 20 (83.3%) of the 24 primary rcc cell cultures. Treatment of acHn human rcc cells with anti-Fas mab in combination with vinblastine, interferon-· or interferon-Á did not affect telomerase activity. However, a combination of anti-Fas mab and doxorubicin resulted in marked down-regulation of telomerase activity in conjunction with a synergistic cytotoxicity. This inhibitory effect on telomerase activity was also observed in 16 telomerase-positive primary RCC cell cultures. These findings suggest that telomerase may be a promising molecular target for combination therapy using biological response modifiers and doxorubicin for rcc.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of telomerase activity in renal cell carcinoma cells by doxorubicin and anti-Fas monoclonal antibody

Jin¹

2009

Mol Med Rep

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“…The cellular toxicity of the extractson Vero cells were evaluated by means of the Cell Proliferation Kit (Biological Industries, Beit Haemek, Israel) in 96 well flat bottomed microtiter plates 19 .…”

Section: Cellular Toxicitymentioning

confidence: 99%

Untitled

2018

IJPSR

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ABSTRACT:To find new antiviral agents from natural sources, methanol and aqueous extracts of leaves and fruits of Ribes uvacrispa and Ribes multiflorum naturally grown in Turkey were investigated as in vitro to reveal their antiviral activities against Herpes simplex virus type 1. All experiments were performed in 96-well plates and results were measured with XTT-based colorimetric assay. Results demonstrated that all extracts has no cytotocic effect on vero cells at 10000 µg/mL, while they also exhibited anti-HSV-1 activity with different percentages of protection (varying between 2.65% -50.40%) in 10000 µg/mL which was at the highest concentration in Vero cells. 50% effective concentrations (EC 50 ) of the extracts which were determined having percentage of protection against HSV-1 at concentrations lower than 10000 µg/mL were calculated using GraphPad Prism Version 5.03 statistics program with non-linear regression analysis. These extracts were determined to have EC 50 values ranging between 9710 -70600 µg/mL and selectivity index (SI) are ranging between 0.14 -1.03. On the basis of these results we believe that it would be worthwhile expanding these studies to include additional species of Turkish plants.

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“…Traditionally, BL has been treated with chemotherapy, such as etoposide, doxorubicin or cytarabine, which are known to induce cancer cell death by apoptosis [12,13]. However, EBV reactivation has been observed following chemotherapy and may result either from a direct effect of the chemotherapeutic agent on the virus or an indirect effect on the cells.…”

Section: Introductionmentioning

confidence: 99%

Treatment of Akata EBV-Positive Cells with Doxorubicin Causes More EBV Reactivation than Treatment with Etoposide

Lima

Seca²,

Brás³

et al. 2011

Chemotherapy

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Background/Aims: EBV has been associated with Burkitt lymphoma (BL). It establishes a latent infection but its reactivation has been observed in patients receiving long-term chemotherapy. The effect of doxorubicin on virus reactivation has been described previously, but the effect of etoposide or cytarabine on EBV reactivation has not been reported in the literature. The aim of this work was to carry out such a study. Methods: Akata EBV-positive cell lines were treated with etoposide, doxorubicin or cytarabine. Viable cells were analyzed by trypan blue, programmed cell death by TUNEL assay, mRNA levels by RT-PCR and cellular or viral proteins by Western blot. Viruses were visualized by electron microscopy. Results: All of the studied drugs caused cell death by apoptosis. Comparing the effect of etoposide and doxorubicin (at their IC₅₀) in the EBV-positive cells, etoposide caused less EBV reactivation than doxorubicin. Cytarabine apparently did not reactivate EBV. Conclusion: When treating Akata EBV-positive cells with the respective IC₅₀ of the following drugs, etoposide induced less EBV reactivation than doxorubicin, and cytarabine apparently did not induce EBV reactivation.

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Effect of Doxorubicin on Telomerase Activity and Apoptotic Gene Expression in Doxorubicin-Resistant and -Sensitive MCF-7 Cells

Cited by 19 publications

References 41 publications

Inhibition of telomerase activity in renal cell carcinoma cells by doxorubicin and anti-Fas monoclonal antibody

Inhibition of telomerase activity in renal cell carcinoma cells by doxorubicin and anti-Fas monoclonal antibody

Untitled

Treatment of Akata EBV-Positive Cells with Doxorubicin Causes More EBV Reactivation than Treatment with Etoposide

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