Effect of down-regulated miR-15b-5p expression on arrhythmia and myocardial apoptosis after myocardial ischemia reperfusion injury in mice

Niu, Siquan; Xu, Liang; Yang, Yuan; Yang, Shaohua; Ning, Hong-Jie; Qin, Xutan; Xin, Pengcheng; Yuan, Dandan; Jiao, Jingmei; Zhao, Yujie

doi:10.1016/j.bbrc.2020.06.111

Cited by 16 publications

(12 citation statements)

References 23 publications

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“…It has been documented that the increased expression of miR-30e, miR-193b, miR-135a, miR-138, miR-140, miR-145, miR-15b-5p, miR-483 and miR-590-3p could suppress cardiomyocyte apoptosis induced by MIRI. Similar findings were observed following the decreased expression of miR-1, miR-327, miR-128-1-5p, miR-129-5p, miR-489 and miR-421 (4,13,23,(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). Zhang et al (23) indicated that miR-193b overexpression may reduce apoptosis after MIRI and alleviate IR-induced myocardial injury by targeting mastermind-like 1, which is a transcriptional coactivator in the Notch signaling pathway.…”

Section: Role Of Mirnas In Mirisupporting

confidence: 53%

Involvement of non‑coding RNAs in the pathogenesis of myocardial ischemia/reperfusion injury (Review)

Fan

et al. 2021

Int J Mol Med

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Myocardial ischemia/reperfusion injury (MIRI) may cause myocardial stunning, reperfusion arrhythmia, no-reflow phenomenon and lethal reperfusion injury, which has a significant effect on the prognosis of patients undergoing thrombolytic agent therapy and percutaneous coronary intervention. Increasing evidence suggests that apoptosis, innate inflammation, oxidative stress, calcium overload and autophagy are involved in the pathogenesis of MIRI. Recent advancements in RNA sequencing technologies and genome-wide analyses led to the finding of small non-coding RNAs (ncRNAs). ncRNAs modulate cellular processes such as signal transduction, transcription, chromatin remodeling and post-transcriptional modification. The effects of ncRNAs on cellular biology is more considerable than initially expected, and thus ncRNAs have gained increasing attention and focus in modern medical research. There are several types of ncRNAs, such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), which have been shown to regulate gene expression at the transcription, post-transcription and epigenetic levels. dysregulation of ncRNAs, including miRNAs, lncRNAs and circRNAs, may participate in the molecular mechanisms of MIRI. The present review summarizes the characteristics and biological roles of miRNAs, lncRNAs and circRNAs, with particular emphasis on their role in MIRI, which show the novel complexity of ischemic hearts and may offer valuable insights into the pathogenesis of MIRI. Contents1. Introduction 2. Biological functions of microRNAs (miRs/miRNAs) 3. Role of miRNAs in myocardial ischemia/reperfusion injury (MIRI) 4. Biological functions of long non-coding RNAs (lncRNAs) 5. Role of lncRNAs in MIRI 6. Biological functions of circular RNAs (circRNAs) 7. Role of circRNAs in MIRI 8. conclusion

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Section: Role Of Mirnas In Mirisupporting

confidence: 53%

Involvement of non‑coding RNAs in the pathogenesis of myocardial ischemia/reperfusion injury (Review)

Fan

et al. 2021

Int J Mol Med

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“…This study also investigated the targets of 11 miRNAs by the miRNA target database and found that 152 genes were also confirmed as DEGs in GSE67308. miR-363-3p has been identified as the diagnostic biomarker for MI/R injury, and upregulated miR-15b-5p, miR-34a-5p, and miR-146a-5p have been also confirmed to play promoters roles in the progression of MI/R injury [ 18 – 20 ]. Moreover, miR-92a-3p and miR-27b-3p have been confirmed to have connection with I/R injury or myocardial injury.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the miRNA-mRNA Regulatory Network Reveals the Biomarker Genes in the Progression of Myocardial Ischemic Reperfusion

Li¹,

Chen²

2022

Journal of Healthcare Engineering

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Objective. Cardiac injury induced by myocardial ischemic reperfusion (MI/R) is still an intractable question in clinical, and it has been confirmed as a major reason for the development of cardiovascular disease. Bioinformatics analysis has been widely used for revealing the pathogenic mechanism of diseases. This study attempted to identify the biomarkers and reveal the regulation mechanism of MI/R injury via bioinformatics analysis. Methods. The GSE67308 and GSE74951 were obtained from the GEO database. The datasets were analyzed with GEO2R tool, and the genes with |logFC| > 2 and p value <0.05 were identified as the differentially expressed genes (DEGs). The enrichment analysis of the DEGs was performed with the DAVID database and R language. Moreover, the protein-protein interaction (PPI) network of DEGs was performed with the STRING database and then visualized with Cytoscape. Result. The results showed that 195 downregulated mRNAs and 240 downregulated mRNAs were found in GSE67308, and 11 miRNAs were found in GSE7495. 152 common genes were screened in DEGs of GSE67308 and the targets of 11 miRNAs in GSE7495. Moreover, the enrichment analysis showed that the common genes were related with inflammatory response, immune response, PI3K/AKT, NF-κB, and TNF pathways. Besides, mmu-miR-92a-3p and mmu-miR-27b-3p were identified as the hubs miRNAs, and TNF, IL1B, and IFG1 were screened as the key nodes. Conclusion. This study established a miRNA-mRNA network for cardiac injury induced by MI/R and provided the evidence concerning the molecular mechanism of MI/R injury, which provided some reference for MI/R treatment.

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“…In addition, its main active components, such as triterpenoids and polysaccharides, have been proven to play an important role in the treatment of arrhythmia (Wu et al, 2014;Li et al, 2015). Studies have indicated that cardiomyocyte apoptosis plays an important role in arrhythmia, and the severity of apoptosis is positively correlated with the arrhythmia score (Niu et al, 2020). In addition, zebrafish heart congestion, an extended SV-BA distance, pericardial oedema and other cardiac morphological changes can cause heart damage and arrhythmia.…”

Section: Discussionmentioning

confidence: 99%

Poria cum Radix Pini Rescues Barium Chloride-Induced Arrhythmia by Regulating the cGMP-PKG Signalling Pathway Involving ADORA1 in Zebrafish

et al. 2021

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The traditional Chinese medicine Poria cum Radix Pini (PRP) is a fungal medicinal material that has been proven to play an important role in the treatment of arrhythmia. However, the mechanism of its effect on arrhythmia is still unclear. In this study, network pharmacology and metabolomics correlation analysis methods were used to determine the key targets, metabolites and potential pathways involved in the effects of PRP on arrhythmia. The results showed that PRP can significantly improve cardiac congestion, shorten the SV-BA interval and reduce the apoptosis of myocardial cells induced by barium chloride in zebrafish. By upregulating the expression of the ADORA1 protein and the levels of adenosine and cGMP metabolites in the cGMP-PKG signalling pathway, PRP can participate in ameliorating arrhythmia. Therefore, we believe that PRP shows great potential for the treatment of arrhythmia.

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Effect of down-regulated miR-15b-5p expression on arrhythmia and myocardial apoptosis after myocardial ischemia reperfusion injury in mice

Cited by 16 publications

References 23 publications

Involvement of non‑coding RNAs in the pathogenesis of myocardial ischemia/reperfusion injury (Review)

Involvement of non‑coding RNAs in the pathogenesis of myocardial ischemia/reperfusion injury (Review)

Analysis of the miRNA-mRNA Regulatory Network Reveals the Biomarker Genes in the Progression of Myocardial Ischemic Reperfusion

Poria cum Radix Pini Rescues Barium Chloride-Induced Arrhythmia by Regulating the cGMP-PKG Signalling Pathway Involving ADORA1 in Zebrafish

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