Effect of DNA methylation profile on OATP3A1 and OATP4A1 transcript levels in colorectal cancer

Rawłuszko-Wieczorek, Agnieszka Anna; Horst, Nikodem; Horbacka, Karolina; Bandura, Artur Szymon; Świderska, Monika; Krokowicz, Piotr; Jagodzińśki, Paweł P.

doi:10.1016/j.biopha.2015.08.026

Cited by 16 publications

(7 citation statements)

References 43 publications

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“…In this study, also the effect of the DNA methylation inhibitor, decitabine on the methylation and expression of PPP1CA , BTG2 , and PTEN genes, in association with changes in miR‐125b, miR‐17, and miR‐181b levels, was investigated. Some previous studies reported these miRNAs play important roles in this type of leukemia …”

Section: Discussionmentioning

confidence: 97%

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The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR‐125b, miR‐17, and miR‐181b in NALM6 cell line

Vafadar

Mokaram

Erfani

et al. 2019

J of Cellular Biochemistry

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Precursor B-cell acute lymphoblastic leukemia (B-ALL) is the most prevalent pediatric cancer. DNA methylation and changes in the microRNAs (miRNAs) expression are known to be important causes of B-ALL. Decitabine as a DNA methyltransferase inhibitor agent is able to induce hypomethylation in several tumor suppressor genes. Much evidence has proven BTG2, PPP1CA, and PTEN act as tumor suppressor genes in many malignancies. In this case control study, the messenger RNA (mRNA) expression of PPP1CA, BTG2, and PTEN genes using quantitative real-time polymerase chain reaction (rRT-PCR) in Nalm6 cell line and five patients suffer from ALL with mean age 5.6 years were determined in compare with seven normal healthy donors age and sex matched. qRT-PCR analysis revealed that the expression levels of PPP1CA, BTG2, and PTEN genes were significantly decreased in Nalm6 ([FC] = 0.46, [FC] = 0.046, [FC] = 0.54) and according to the Methylation-specific PCR (MSP) analysis, these genes were hypermethylated in Nalm6. In next step, the effects of decitabine treatment on the methylation and expression of these genes in association with changes in miR-125b, miR-17, and miR-181b expression levels were evaluated in optimal concentration 2.5 µM of decitabine. Our data showed that decitabine is able to restore the expression levels of aforementioned genes and downregulate expression levels of oncomiRs; including miR-125b, miR-17, and miR-181b in Nalm6 cell line. Therefore, it seems that decitabine can be used as a potential drug for the first line treatment of patients with B-ALL, but further in vivo investigation is necessary. K E Y W O R D S B-ALL, decitabine, DNA methylation, microRNA, NALM6 J Cell Biochem. 2019;120:13156-13167. wileyonlinelibrary.com/journal/jcb 13156 |

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Section: Discussionmentioning

confidence: 97%

“…Some previous studies reported these miRNAs play important roles in this type of leukemia. 28,30,31 The effect of decitabine on cell proliferation was carried out using the MTT assay. The IC-50 of decitabine was 2.5 μM in Nalm6 cells, upon exposure for 96 hours.…”

Section: Discussionmentioning

confidence: 99%

The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR‐125b, miR‐17, and miR‐181b in NALM6 cell line

Vafadar

Mokaram

Erfani

et al. 2019

J of Cellular Biochemistry

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“…In addition, the expression of SLCO4A1 is strongly increased in ovarian cancers [ 16 ]. Interestingly, organic anion transporting polypeptides, uptake transporters of the solute carrier family, are found to be elevated in diverse cancers, such as colorectal cancer and pancreatic cancer [ 17 , 18 ]. Moreover, the study showed that SLCO4A1 was downregulated by miR-150-3p, which can inhibit colon cancer stem cell progression.…”

Section: Discussionmentioning

confidence: 99%

LncRNA SLCO4A1-AS1 modulates colon cancer stem cell properties by binding to miR-150-3p and positively regulating SLCO4A1

Song

et al. 2021

Laboratory Investigation

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Long non-coding RNAs (lncRNAs) play important roles in a range of different human cancers. However, the role of lncRNA solute carrier organic anion transporter family member 4A1-AS1 (SLCO4A1-AS1) in colon cancer remains enigmatic. Hence, we aimed to explore the specific role of SLCO4A1-AS1 in colon cancer stem cells. Colon cancer-related differentially expressed lncRNA and mRNA were screened using microarray-based analysis, and the expression of SLCO4A1-AS1 and SLCO4A1 in colon cancer tissues was determined using reverse transcription quantitative polymerase chain reaction and western blot analysis. The interaction among SLCO4A1-AS1, microRNA-150-3p (miR-150-3p) and SLCO4A1 was verified using dual-luciferase reporter assay, RNA immunoprecipitation and RNA pull-down. Moreover, SLCO4A1-AS1, miR-150-3p and/or SLCO4A1 were overexpressed or depleted in colon cancer cells to detect their effects on migration, invasion, sphere formation, apoptosis and tumorigenesis abilities of colon cancer stem CD133+CD44+ cells using both in vitro and in vivo assays. SLCO4A1-AS1 and SLCO4A1 were screened as the differentially expressed lncRNA and mRNA in colon cancer tissues. SLCO4A1-AS1 was confirmed to competitively bind to miR-150-3p to elevate SLCO4A1 expression. Moreover, knockdown of SLCO4A1-AS1 decreased SLCO4A1 expression, thus inhibiting cell migration, invasion, sphere formation, and tumorigenesis abilities and enhancing the apoptosis of CD133+CD44+ cells. Collectively, these findings provide evidence demonstrating that depleting SLCO4A1-AS1 competitively binds to miR-150-3p, which downregulates SLCO4A1 expression, thus hindering colon cancer progression.

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“… 36 DNA hypermethylation in the SLCO3A1 promoter region was detected in a small subset of colorectal cancer patients and in HCT116 and Caco-2 cell lines. 37 Higher methylation level of SLCO3A1 (also known as OATP3A1) was associated with shorter post-treatment survival of patients with chronic lymphocytic leukaemia. 38 Univariate Cox regression and Kaplan–Meier survival analyses showed that high methylation located in NTRK3 gene was significantly associated with poor prognosis in patients with esophageal squamous cell carcinoma (HR = 1.79).…”

Section: Discussionmentioning

confidence: 99%

Signature Panel of 11 Methylated mRNAs and 3 Methylated lncRNAs for Prediction of Recurrence-Free Survival in Prostate Cancer Patients

Cai

Yang

Chen

et al. 2021

PGPM

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Background: Radical prostatectomy is the main treatment for prostate cancer (PCa), a common cancer type among men. Recurrence frequently occurs in a proportion of patients. Therefore, there is a great need to early screen those patients to specifically schedule adjuvant therapy to improve the recurrence-free survival (RFS) rate. This study aims to develop a biomarker to predict RFS for patients with PCa based on the data of methylation, an important heritable contributor to carcinogenesis. Methods: Methylation expression data of PCa patients were downloaded from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus database (GSE26126), and the European Bioinformatics Institute (E-MTAB-6131). The stable co-methylation modules were identified by weighted gene co-expression network analysis. The genes in modules were overlapped with differentially methylated RNAs (DMRs) screened by MetaDE package in three datasets, which were used to screen the prognostic genes using least absolute shrinkage and selection operator analyses. The prognostic performance of the prognostic signature was assessed by survival curve analysis. Results: Five co-methylation modules were considered preserved in three datasets. A total of 192 genes in these 5 modules were overlapped with 985 DMRs, from which a signature panel of 11 methylated messenger RNAs and 3 methylated long non-coding RNAs was identified. This signature panel could independently predict the 5-year RFS of PCa patients, with an area under the receiver operating characteristic curve (AUC) of 0.969 for the training TCGA dataset and 0.811 for the testing E-MTAB-6131 dataset, both of which were higher than the predictive accuracy of Gleason score (AUC = 0.689). Also, the patients with the same Gleason score (6-7 or 8-10) could be further divided into the high-risk group and the low-risk group. Conclusion: These results suggest that our prognostic model may be a promising biomarker for clinical prediction of RFS in PCa patients.

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Effect of DNA methylation profile on OATP3A1 and OATP4A1 transcript levels in colorectal cancer

Cited by 16 publications

References 43 publications

The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR‐125b, miR‐17, and miR‐181b in NALM6 cell line

The effect of decitabine on the expression and methylation of the PPP1CA, BTG2, and PTEN in association with changes in miR‐125b, miR‐17, and miR‐181b in NALM6 cell line

LncRNA SLCO4A1-AS1 modulates colon cancer stem cell properties by binding to miR-150-3p and positively regulating SLCO4A1

Signature Panel of 11 Methylated mRNAs and 3 Methylated lncRNAs for Prediction of Recurrence-Free Survival in Prostate Cancer Patients

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