Effect of DL-α-lipoic acid on the status of lipid peroxidation and protein oxidation in various brain regions of aged rats

Arivazhagan, Palaniyappan; Thilakavathy, Thangaswamy; Ramanathan, Kadirvel; Sundaram, Kumaran; Panneerselvam, Chinnakkannu

doi:10.1016/s0955-2863(02)00217-6

Cited by 28 publications

(18 citation statements)

References 34 publications

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“…Thiols are thought to play a pivotal role in protecting cells against lipid peroxidation. Also lipoic acid extends its role in scavenging free radicals thereby protecting cells from lipid peroxidation (Arivazhagan and Panneerselvam 2002). Thus the present observation shows that carnitine and lipoate administration eventually resulted in decrease in lipid peroxidation levels.…”

Section: Group Isupporting

confidence: 67%

Oxidative Stress and DNA Single Strand Breaks in Skeletal Muscle of Aged Rats: Role of Carnitine and Lipoicacid

Sundaram

Panneerselvam

2006

Biogerontology

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The exposure of biological system to various conditions of oxidative stress is the major contributor for aging process. Oxidative stress in turn increases the cellular levels of oxidatively modified proteins, lipids and nucleic acids resulting in a loss of physical activity and metabolic integrity. In this study, we evaluated the role of L-carnitine and DL-alpha-lipoic acid in minimizing oxidant generation and macromolecular damage in skeletal muscle of aged rats. We found that the oxidant generation was increased in aged rat skeletal muscle when compared to young rats. There was a simultaneous increase in the levels of lipid peroxidation, protein carbonyl content and DNA strand breaks in aged rat skeletal muscle. Administration of L-carnitine (300 mg/kg body wt/day) and DL-alpha-lipoic acid (100 mg/kg body wt/day) to aged rats for 30 days, decreased the oxidant generation, lipid peroxidation, protein carbonylation and DNA strand breaks. We concluded that co-administration of carnitine and lipoic acid to aged rats has the potential to prevent oxidative stress mediated macromolecular damage in skeletal muscle of aged rats by their putative role as efficient antioxidants.

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Section: Group Isupporting

confidence: 67%

Oxidative Stress and DNA Single Strand Breaks in Skeletal Muscle of Aged Rats: Role of Carnitine and Lipoicacid

Sundaram

Panneerselvam

2006

Biogerontology

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“…Reduced lipid peroxidation following α-LA administration has been observed to be associated with a protective effect against adriamycin-induced nephrotoxicity and cardiotoxicity in rats (34,35). Additional studies have found that α-LA treatment results in a decreased MDA level in the plasma (36), liver, kidney (17) and different regions of the brain (37).…”

Section: Discussionmentioning

confidence: 94%

Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats

et al. 2013

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Background: Oxidative stress is believed to be an important factor in the pathogenesis of acute lung injury (ALI).Aims: The aim of this study was to investigate the possible protective role of alpha-lipoic acid (α-LA) on oleic acid (OA)-induced ALI in rats.Study Design: Animal experiment.Methods: A total of thirty-five rats were divided into five groups in the study. Group 1 served as a control group. Rats in Group 2 (α-LA) were administered α-LA intraperitoneally at a dose of 100 mg/kg body weight (BW). Rats in Group 3 (OA) were administered OA intravenously at a dose of 100 mg/kg BW. In Group 4 (pre-OA-α-LA), α-LA was given 15 minutes prior to OA infusion, and in Group 5 (post-OA-α-LA), α-LA was given two hours after OA infusion. Four hours after the OA infusion, rats were decapitated. Blood samples were collected to measure serum levels of malondialdehyde (MDA) and glutathione (GSH), and the levels of activity for superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Lung tissue samples were taken for histopathological examination.Results: Exposure to OA resulted in increases in serum MDA levels (p<0.001), as well as histopathological lesions in lung tissue, and decreases in CAT (p<0.05), GSH-Px (p<0.05) activities and GSH (p<0.05) levels. On the other hand, MDA levels were decreased significantly (p<0.001), while CAT (p<0.05), GSH-Px (p<0.01) activities and GSH (p<0.05) levels were increased significantly in the pre-OA-α-LA group compared with the OA group.Conclusion: α-LA was found to lessen oxidative stress and to have positive effects on antioxidants in cases of OA-induced ALI. In conclusion, α-LA appears to have protective effects against ALI and potential for the prevention of ALI.

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“…To our knowledge, there is only one study evaluating LA supplementation in ageing brain in terms of PCO. 37 Arivazhagan et al 37 reported that PCO levels were significantly decreased in brain tissue of aged Wistar rats given LA supplementation. Although we used the same protocol as Arivazhagan et al, 37 we found a contrasting effect of a LA on protein oxidation.…”

Section: Discussionmentioning

confidence: 99%

“…37 Arivazhagan et al 37 reported that PCO levels were significantly decreased in brain tissue of aged Wistar rats given LA supplementation. Although we used the same protocol as Arivazhagan et al, 37 we found a contrasting effect of a LA on protein oxidation. In the present study, PCO, and NT levels were increased significantly inaged Sprague-Dawley rats receiving LA supplementation compared to aged control rats.…”

Section: Discussionmentioning

confidence: 99%

“…In this respect, our findings concerning the brain LHP levels support the findings of other authors. 33,37 Detection of lipid peroxidation has relied largely on indirect methods, i.e., analysis of secondary or end-products derived from hydroperoxides such as malondialdehyde and TBARS. The malondialdehyde test is very sensitive but poor in specificity.…”

Section: Discussionmentioning

confidence: 99%

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Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat

Kayalı

Çakatay

Akçay

et al. 2005

Cell Biochem. Funct.

176

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In the present study, we investigated whether DL-alpha-lipoic acid (LA) supplementation could have prooxidant or antioxidant effects on oxidative protein damage parameters such as protein carbonyl (PCO), nitrotyrosine (NT), advanced oxidation protein products (AOPP), and protein thiol (P-SH), as well as oxidative stress parameters such as total thiol (T-SH), non-protein thiol (Np-SH), and lipid hydroperoxide (LHP) in the brain and the skeletal muscle tissue of aged rats. PCO, and NT levels were increased, AOPP and P-SH levels were not changed in the brain tissue of aged rats given LA supplementation. On the other hand, TSH, Np-SH, and LHP levels were decreased in the brain tissue of aged rats given LA supplementation. The levels of the same parameters were not significantly different in the skeletal muscle tissue of aged rats given LA supplementation. The increased levels of protein oxidation markers such as PCO, and NT in the brain tissue of LA-supplemented aged rats compared with non-supplemented aged rats may suggest that oxidative protein damage is increased in LA-supplemented aged rats. We assume that an explanation for our findings regarding LA supplementation on protein oxidation markers in the brain tissue of aged rats may be due to the prooxidant effects of LA. Depending on post-mitotic tissue type and dosage of LA, the prooxidant effects of LA supplementation, should be considered in future studies.

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Effect of DL-α-lipoic acid on the status of lipid peroxidation and protein oxidation in various brain regions of aged rats

Cited by 28 publications

References 34 publications

Oxidative Stress and DNA Single Strand Breaks in Skeletal Muscle of Aged Rats: Role of Carnitine and Lipoicacid

Oxidative Stress and DNA Single Strand Breaks in Skeletal Muscle of Aged Rats: Role of Carnitine and Lipoicacid

Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats

Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat

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