Effect of divalent cations on the porcine kidney cortex membrane-bound form of dipeptidyl peptidase IV

Pascual, Isel; Gómez, Hansel; Pons, Tirso; Chappe, Mae; Vargas, Miguel; Valdés, Gilberto; López, A. O.; Saroyán, Angélika; Charlí, Jean-Louis; Chávez, María Á.

doi:10.1016/j.biocel.2010.11.006

Cited by 7 publications

(7 citation statements)

References 54 publications

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“…The optimal activity was similar to a purified DPP-IV from porcine kidney. 15) As expected as for the inhibition assay, the enzymatic activity of rDPP-IV was significantly reduced by 80 or 60% in the presence of sitagliptin (a DPP-IV inhibitor) or PMSF (a serine protease inhibitor), but was not apparently affected by iodoacetamide (a cysteine protease inhibitor) (Fig. 6).…”

Section: Affinity Purification Glycostaining and Lc/ms-ms Confirmatisupporting

confidence: 77%

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Functional Expression and Characterization of Dipeptidyl Peptidase IV from the Black-Bellied HornetVespa basalisin Sf21 Insect Cells

Hsieh

Tzen²,

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: Affinity Purification Glycostaining and Lc/ms-ms Confirmatisupporting

confidence: 77%

“…5), similar to previously reports. 15,21,22) On the basis of some reports, 15,23,24) we speculate that the optimal activity for rDPP-IV involves important physiologic mechanisms of V. basalis, such as defense or antagonist of the external stress.…”

Section: Discussionmentioning

confidence: 80%

Functional Expression and Characterization of Dipeptidyl Peptidase IV from the Black-Bellied HornetVespa basalisin Sf21 Insect Cells

Hsieh

Tzen²,

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…Biochemical inhibition of either furin or CyPB prevents exposure of the RG-1 epitope and causes a blockage of HPV16 infection at late stages postentry (4,49). Bac has been reported to have pleiotropic effects, including the inhibition of certain proteases (3,35,46,81), and it is conceivable that Bac could have some inhibitory effects on either furin or CyPB, thereby blocking infection at a late stage by preventing exposure of the RG-1 epitope. We therefore tested the effects of Bac on RG-1 epitope exposure by immunostaining with the RG-1 antibody at 4 h postinfection.…”

Section: Bacitracin Inhibition Of Hpv Infectionmentioning

confidence: 99%

Opposing Effects of Bacitracin on Human Papillomavirus Type 16 Infection: Enhancement of Binding and Entry and Inhibition of Endosomal Penetration

Campos

Chapman

Deymier

et al. 2012

J Virol

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e Cell invasion by human papillomavirus type 16 (HPV16) is a complex process relying on multiple host cell factors. Here we describe an investigation into the role of cellular protein disulfide isomerases (PDIs) by studying the effects of the commonly used PDI inhibitor bacitracin on HPV16 infection. Bacitracin caused an unusual time-dependent opposing effect on viral infection. Enhanced cellular binding and entry were observed at early times of infection, while inhibition was observed at later times postentry. Bacitracin was rapidly taken up by host cells and colocalized with HPV16 at late times of infection. Bacitracin had no deleterious effect on HPV16 entry, capsid disassembly, exposure of L1/L2 epitopes, or lysosomal trafficking but caused a stark inhibition of L2/viral DNA (vDNA) endosomal penetration and accumulation at nuclear PML bodies. ␥-Secretase has recently been implicated in the endosomal penetration of L2/vDNA, but bacitracin had no effect on ␥-secretase activity, indicating that blockage of this step occurs through a ␥-secretase-independent mechanism. Transient treatment with the reductant ␤-mercaptoethanol (␤-ME) was able to partially rescue the virus from bacitracin, suggesting the involvement of a cellular reductase activity in HPV16 infection. Small interfering RNA (siRNA) knockdown of cellular PDI and the related PDI family members ERp57 and ERp72 reveals a potential role for PDI and ERp72 in HPV infection. Human papillomaviruses (HPVs) are one of the most common sexually transmitted infections in the world. HPVs are small 55-nm icosahedral nonenveloped double-stranded DNA (dsDNA) viruses that replicate in differentiating cutaneous and mucosal epithelium. Infection of mucosal epithelium by oncogenic HPV genotypes can lead to cervical, anogenital, and other head and neck cancers. HPV type 16 (HPV16) is the most common of the high-risk types and is alone responsible for over 50% of cervical cancers worldwide (77). Although HPVs have been known to be the etiological agent of cervical cancer for nearly 30 years, and despite intensive research in recent years, the infectious entry pathway of HPV16 is still not well defined. Our current understanding of HPV cellular invasion reveals a complex and prolonged process, complicated by differences between in vitro cell culture systems and the recently described in vivo mouse cervicovaginal challenge model (33,37,50,62).The HPV capsid is assembled from 360 molecules of the L1 protein, arranged as 72 pentamers. L1 monomers from neighboring pentamers are disulfide bonded to each other as dimers and trimers, providing stability to the capsid (45). The minor capsid protein L2 is localized within a central cavity beneath the L1 pentamers. L2 can be present at a maximum stoichiometry of one L2 molecule per L1 pentamer or 72 molecules per virion; however, most preparations of virus contain submaximal levels of L2, typically 20 to 25 copies per virion (6). Packaged within the capsid is the ϳ8-kb viral genome (viral DNA [vDNA]), condensed as chromatin with cellu...

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“…These findings suggest that the binding of sCD26 to the spike (S) glycoprotein may also depend on T-cell population.Although SARS-CoV-2 may primarily enter the cells of the lungs, the small bowel may also be an important site of entry or interaction site, as the enterocytes are rich in angiotensin converting enzyme (ACE)-2 receptors[33]. Duodenal mucosa expresses mCD26 particularly on the apical site of the brush border epithelial cells where Tcells present and bind with many divalent metals including Fe + , Ca + , Co + , Zn + and Hg +[57]. Particularly, Iron (Fe + ) play an important role in inflammation and causes disbalanece and hyperferritinaemia in Covid-19[58].…”

mentioning

confidence: 99%

Investigation of CD26, a potential SARS-CoV-2 receptor, as a biomarker of age and pathology

et al. 2020

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Objective: In some individuals, coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection leads to a variety of serious inflammatory symptoms, including blood clotting and acute respiratory distress. Death due to COVID-19 shows a steep rise in relation to age. Comorbidities such as type 2 diabetes mellitus (T2DM), hypertension, and cardiovascular disease also increase susceptibility. It has been reported that T-cell regulatory dipeptidyl peptidase 4 (DPP4; cluster of differentiation 26 (CD26)) binds to the external spike (S) glycoprotein of SARS-CoV-2 as a receptor, for the viral entry into the host cell. CD26 is expressed on many cells, including T and natural killer (NK) cells of the immune system, as a membrane-anchored form. A soluble form (sCD26) is also found in the blood plasma and cerebrospinal fluid (CSF). Approach and results: To investigate a possible relationship between sCD26 levels, age and pathology, serum samples were collected from control, T2DM and age-related dementia (ARD) subjects. A significant reduction in serum sCD26 levels was seen in relation to age. ARD and T2DM were also associated with lower levels of sCD26. The analysis of blood smears revealed different cellular morphologies: in controls, CD26 was expressed around the neutrophil membrane, whereas in T2DM, excessive sCD26 was found around the mononucleated cells (MNCs). ARD subjects had abnormal fragmented platelets and haemolysis due to low levels of sCD26. Conclusions: These findings may help to explain the heterogeneity of SARS-CoV-2 infection. High serum sCD26 levels could protect from viral infection by competively inhibiting the virus binding to cellular CD26, whereas low sCD26 levels could increase the risk of infection. If so measuring serum sCD26 level may help to identify individuals at high risk for the COVID-19 infection.

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Effect of divalent cations on the porcine kidney cortex membrane-bound form of dipeptidyl peptidase IV

Cited by 7 publications

References 54 publications

Functional Expression and Characterization of Dipeptidyl Peptidase IV from the Black-Bellied HornetVespa basalisin Sf21 Insect Cells

Functional Expression and Characterization of Dipeptidyl Peptidase IV from the Black-Bellied HornetVespa basalisin Sf21 Insect Cells

Opposing Effects of Bacitracin on Human Papillomavirus Type 16 Infection: Enhancement of Binding and Entry and Inhibition of Endosomal Penetration

Investigation of CD26, a potential SARS-CoV-2 receptor, as a biomarker of age and pathology

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