Effect of dissolved oxygen concentration on microalgal culture in photobioreactors

Kazbar, Antoinette; Cogne, Guillaume; Urbain, Brieuc; Marec, H.; Le-Gouic, B.; Tallec, J.; Takache, Hosni; Ismail, Ali; Pruvost, Jérémy

doi:10.1016/j.algal.2019.101432

Cited by 108 publications

(40 citation statements)

References 42 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, sludge extract additions, which provided iron, carbon, and nitrogen sources, among other nutrients, were evaluated to setback the oxygen dependant activity, which was deduced from monitoring the dissolved oxygen concentration in the medium. Hence, high values of pO 2 % (>70%) indicated no or low-active cellular metabolic activity [25].…”

Section: Use Of Anaerobically Digested Waste Sludge Extract As a Media Supplement For C Sorokiniana Cultivation Under Heterotrophic Condimentioning

confidence: 99%

Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

et al. 2021

View full text Add to dashboard Cite

Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h−1) compared with those reported for the heterotrophic culture of Chlorella were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker’s yeast company) was pretreated to obtain an extract as a media supplement for C. sorokiniana. According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (KFe ~12 mg L−1). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of Chlorella and provide an alternative for the valorization of industrial anaerobic sludge.

show abstract

Section: Use Of Anaerobically Digested Waste Sludge Extract As a Media Supplement For C Sorokiniana Cultivation Under Heterotrophic Condimentioning

confidence: 99%

Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Normal temperatures for the growth of microalgae ranged between 25 and 30°C; an increment in the temperature affects the lipid production; at higher temperatures saturated free fatty acids are produced, while low temperatures favor unsaturated free fatty acid formation [15]. High concentration of O 2 can build up in closed PBR; if this happens photosynthesis can be damaged by decreasing microalgae growth, and an improvement in the PBR should be implemented as an effective gas exchange [16].…”

Section: Main Problems In Closed Photobioreactors: Light Supply Temperature and Oxygen Accumulationmentioning

confidence: 99%

Microalgae Cultivation for Secondary Metabolite Production

Márquez-Rocha¹,

Palma‐Ramírez²,

García-Alamilla³

et al. 2020

Microalgae - From Physiology to Application

View full text Add to dashboard Cite

Microalgae including cyanobacteria have been recognized as an excellent source of fine chemicals, renewable fuels, vitamins, and proteins and usually are found in health food stores around the world. However, the accumulation of these compounds generally occurs at end of the exponential growth phase; furthermore, biomass density in cultivation commonly is low. Open cultures have been used for pigment, biofuels, and biomass production, but these types of culture system are not a good choice for the production of fine chemicals, due to contamination problems and the expensive production costs. Closed photobioreactors can be operated in a continuous cultivation providing an increase on biomass density and contamination-free condition and generally working at a maximum growth rate under specific conditions; besides, these systems can recycle the consumed culture medium at least three times before a new enriched medium is supplied, generating a more cost-effective production system. In addition, microalgae metabolism can be manipulated to provoke a specific secondary metabolite accumulation by the addition of organic carbon source or changing light intensity or both. In other words, photobioreactors can operate in continuous mode, with efficient light supply and the supplementation of organic carbon source to produce fine biochemicals such as anticancer, antibacterial, antioxidant, lectins, antiviral compounds, and biofuels.

show abstract

“…For the improvement of algal productivity, various components of algal cultivation have been intensively studied, including control and optimization of pH, culture medium, temperature, nutrient supply, and reactor design (Yew et al, 2019). While the high-density microalgal culture in a closed reactor is advantageous for high-quality biomass and low harvesting cost (Richmond., 2013), photosynthetic oxygen easily accumulates and inhibit the algal growth (Weissman et al, 1988;Kazbar et al, 2019). For example, the dissolved oxygen concentration exceeded 70 mg L −1 (more than 6 times the air saturation) in the outdoor Arthrospira platensis culture, at which the chlorophyll synthesis decreased to less than half (Torzillo et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Effect of Membrane Hydrophobicity and Thickness on Energy-Efficient Dissolved Oxygen Removal From Algal Culture

Kishi

Nagatsuka

Toda

2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Removal of dissolved oxygen from algal photobioreactors is essential for high productivity in mass cultivation. Gas-permeating photobioreactor that uses hydrophobic membranes to permeate dissolved oxygen (pervaporation) from its body itself is an energy-efficient option for oxygen removal. This study comparably evaluated the characteristics of various commercial membranes and determined the criteria for the selection of suitable ones for the gas-permeating photobioreactors. It was found that oxygen permeability is limited not by that in the membrane but in the liquid boundary layer. Membrane thickness had a negative effect on membrane oxygen permeability, but the effect was as minor as less than 3% compared with the liquid boundary layer. Due to this characteristic, the lamination of non-woven fabric with the microporous film did not significantly decrease the overall oxygen transfer coefficient. The permeability in the liquid boundary layer had a significantly positive relationship with the hydrophobicity. The highest overall oxygen transfer coefficients in the water-to-air and water-to-water oxygen removal tests were 2.1 ± 0.03 × 10 −5 and 1.39 ± 0.09 × 10 −5 m s −1 , respectively. These values were considered effective in the dissolved oxygen removal from high-density algal culture to prevent oxygen inhibition. Furthermore, hydrophobicity was found to have a significant relationship also with water entry pressure, which needs to be high to avoid culture liquid leakage. Therefore, these results suggested that a microporous membrane with strong hydrophobicity laminated with non-woven fabric would be suitable characteristics for gas-permeating photobioreactor.

show abstract

Effect of dissolved oxygen concentration on microalgal culture in photobioreactors

Cited by 108 publications

References 42 publications

Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production

Microalgae Cultivation for Secondary Metabolite Production

Effect of Membrane Hydrophobicity and Thickness on Energy-Efficient Dissolved Oxygen Removal From Algal Culture

Contact Info

Product

Resources

About