Effect of Disodium Cromoglycate on Lymphocyte Response to Antigen and Mitogen

Abstract

The effect of disodium cromoglycate (DSCG) on the three following in vitro correlates of cell-mediated immunity has been studied: (1) Transformation of human peripheral blood lymphocytes after stimulation by an antigen, purified protein derivative and a mitogen, phytohaemagglutinin; (2) release of macrophage migration inhibitory factor, and (3) cytotoxic factor production, from these stimulated lymphocytes. At two non-toxic concentrations DSCG did not inhibit these three lymphocyte functions. These results suggest that DSCG’s clinical effectiveness does not depend upon modulating cell-mediated immune response. The implications of these findings in relation to present concepts of the cellular action of DSCG and the intracellular control of the initiation of lymphocyte transformation are discussed.

Actions of anticolitic drugs on a guinea-pig model of immune colitis

“…To our knowledge this compound has not previously been investigated in an animal model of colitis. However, experiments have investigated the action of DSCG on in vitro correlates of cell-mediated immunity and have shown it to be without modulatory properties [18]. In addition, in vivo studies in guinea-pigs have examined the ability of DSCG to inhibit the 24 h delayed-type hypersensitivity skin reaction and found it to be devoid of any activity in doses up to 1 mg/kg i.v.…”