Effect of direct-fed microbials on utilization of degradable intake protein in receiving steers

KenneyN., M.; VanzantE., S.; HarmonD., L.; McLeodK., R.

doi:10.4141/cjas-2014-021

Cited by 8 publications

(5 citation statements)

References 34 publications

(38 reference statements)

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“…An alternative hypothesis is that DFM resulted in increased bacterial utilization of ammonia. This hypothesis is supported by growth performance data that suggests that degradable intake protein (DIP) requirements are increased with DFM provision (Kenney et al, 2015). Growth efficiency increased linearly with increasing DIP supply in DFM-fed animals.…”

Section: Rumen Fermentationmentioning

confidence: 83%

“…The first compared CON with DFM containing LAB and the second compared the 2 types of DFM (i.e., LAB vs. combination of LAB/LU). The first comparison was derived from previous work that demonstrated increased cattle growth with Vit-E-Men 10G compared to CON (Kenney et al, 2015). The second comparison was utilized to evaluate the potential effects of the addition of LU bacteria in a DFM.…”

Section: Methodsmentioning

confidence: 99%

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Direct-fed microbials containing lactate-producing bacteria influence ruminal fermentation but not lactate utilization in steers fed a high-concentrate diet1,2

Kenney

Vanzant

Harmon

et al. 2015

Journal of Animal Science

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Direct-fed microbials (DFM) have been shown to improve gain and growth efficiency and also modulate ruminal fermentation. In Exp. 1,72 beef steers were used to compare a lactate-producing bacterial (LAB) DFM consisting primarily of Lactobacillus acidophilus and Enterococcus faecium,and a lactate-producing and lactate-utilizing (LAB/LU) DFM consisting primarily of L. acidophilus and Propionibacterium both fed at 10(9) cfu/d. Steers were fed a corn-based finishing diet for 153 d and then slaughtered for collection of carcass characteristics. In Exp. 2, 12 ruminally cannulated steers were fed acorn-based finishing diet and treated with 10(9) cfu/d of LAB DFM. Rumen fluid was sampled on d 14 and 28 over a 12-h period. Steers were ruminally dosed with a 2-L solution of neutralized DL-lactate (0.56 M)and Cr-EDTA (13.22 M) 3 h postfeeding on d 15 and 29. Ruminal samples were collected at 10- and 20-minintervals for the first and second hour postdosing. No differences (P ≥ 0.14) between control (CON) and LAB for DMI, ADG, growth efficiency, or carcass characteristics were observed. Dry matter intake was greater (P = 0.04) for LAB/LU than LAB from d 0 to 28 but did not differ (P ≥ 0.29) thereafter. Average daily gain was greater (P = 0.04) and efficiency tended(P = 0.06) to be greater for LAB than LAB/LU over the entire 153 d. In Exp. 2, total VFA concentration and molar proportions of butyrate were unaffected(P ≥ 0.24). Molar proportions of acetate exhibited a DFM by hour interaction (P = 0.04); however, on average, molar proportion of acetate was 4.4% greater for DFM. Conversely, DFM did not affect the molar proportion of propionate (P = 0.39). On average,molar proportions of propionate tended to increase(P = 0.07), and acetate tended to decrease (P = 0.07)across days. Mean daily ruminal pH was similar for CON on d 14 and 28, whereas mean pH increased from d 14 to 28 for DFM (DFM × day; P = 0.08).Minimum pH remained unchanged for CON over time but increased from d 14 to 2 for DFM (DFM × day;P = 0.10). Maximum pH decreased from d 14 to 28 in CON but increased over time with DFM (DFM × day;P = 0.05). DL- and L-lactate utilization were unaffected by DFM (P ≥ 0.33) or day (P ≥ 0.50). Although the LAB DFM did not impact growth performance, itd id modulate ruminal fermentation, as evidenced by shifts in ruminal VFA profile and pH; however, DFM did not appear to influence ruminal lactate utilization.

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Section: Rumen Fermentationmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Direct-fed microbials containing lactate-producing bacteria influence ruminal fermentation but not lactate utilization in steers fed a high-concentrate diet1,2

Kenney

Vanzant

Harmon

et al. 2015

Journal of Animal Science

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“…29 Diets were formulated to meet all National Research Council nutrient requirements for steers growing at a rate of 1.0 to 1.25 kg/d, except for degradable intake protein, which was a treatment variable in both years. Steers were stratified by weight.…”

Section: Methodsmentioning

confidence: 99%

Discrimination of healthy versus sick steers by means of continuous remote monitoring of animal activity

Smith¹,

Vanzant²,

Carter³

et al. 2015

ajvr

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“…Konelab analysis (Model 20XTi, Thermo Fisher Scientific, Waltham, MA, USA) was used to determine NH 3 -N concentrations following procedures described previously [18]. DL-lactate was determined by gas chromatography (Model 6890N, Network GC, Agilent Technologies, Santa Clara, CA, USA) following the derivatization of culture broth samples [19,20] using 13 C-lactate as the internal standard.…”

Section: Sample Analysismentioning

confidence: 99%

“…In vitro gas production was determined on days 14 and 28 subsequent to initiation of dietary treatments. These sampling days were chosen based on previous research in which positive effects of DFM on performance were largely limited to the first 28 d of feeding (Kenney et al, 2015a, b) [13,14].…”

Section: Introductionmentioning

confidence: 99%

Direct-Fed Microbials Alter Ruminal in vitro Gas Production and Fermentation with a High-Concentrate Diet

Nm¹,

Es²,

Dl³

et al. 2020

J Anim Sci Res

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By simulating the rumen environment, the in vitro gas production technique allows characterization of DFM mediated changes in the rumen. Previous in vitro gas production experiments have focused primarily on the effects of providing Saccharomyces cerevisiae or combinations of yeast and various Lactobacilli strains and have produced variable results on the impact on gas production and the formation of endpoint fermentation end products [10][11][12]; however, there is limited data on the impact of bacterial DFM on in vitro gas production [2]. This study was designed to differentiate between immediate (in vitro, addition to fermentation vessel) and adaptive (in vivo, consumed for 28 d) effects of LAB containing DFM on ruminal in vitro fermentation of high concentrate substrates, as indicated by alterations in gas production and fermentation end products. Materials and Methods Experimental design and treatmentsAll procedures were approved by the University of Kentucky Institutional Animal Care and Use Committee. Twelve ruminally cannulated Angus steers (initial body weight 385 ± 35 kg) were used in a split-split plot design experiment (Figure 1). The whole plot consisted of steer and dietary DFM provision; the subplot consisted of the in vitro fermentation vessel and media DFM, and within sub-plot

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Effect of direct-fed microbials on utilization of degradable intake protein in receiving steers

Cited by 8 publications

References 34 publications

Direct-fed microbials containing lactate-producing bacteria influence ruminal fermentation but not lactate utilization in steers fed a high-concentrate diet1,2

Direct-fed microbials containing lactate-producing bacteria influence ruminal fermentation but not lactate utilization in steers fed a high-concentrate diet1,2

Discrimination of healthy versus sick steers by means of continuous remote monitoring of animal activity

Direct-Fed Microbials Alter Ruminal in vitro Gas Production and Fermentation with a High-Concentrate Diet

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