Effect of dimethylsulphoxide on the functional characteristics of isolated perfused rat liver

Groufsky, Anne K.; Holland, Robyn K.; Lee, D.

doi:10.1016/0011-2240(78)90064-0

Cited by 3 publications

(3 citation statements)

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“…As with BSA, increasing the P V P concentration in the perfusate decreased the volume of bile produced during a three-hour perfusion, but unlike BSA, it seemed to decrease urea synthesis a little. During the three-hour perfusion with diluted blood, GOT was released at a constant rate, and this observation is different both from those reported above ( Figure 5) and those of other workers (Bartosek ef ulii, 1972;Krone et ulii, 1974 ;NovAkovA et alii, 1975 ;Groufsky et a&, 1977). Nevertheless, the concentration of GOT in the perfusate decreased as the concentration of P V P was increased, but it is not possible to consider this observation on osmotic terms in the absence of a molecular weight range for the PVP.…”

Section: Discussioncontrasting

confidence: 72%

“…Samples of the perfusion fluid (0.4 ml) were collected at timed intervals for analysis of potassium, urea, glucose, and aspartate aminotransferase, E.C. 2.6.1.1, (GOT), the methods having been described previously by Groufsky et a& (1977). Bile production was monitored by collecting the bile into a I-ml syringe the outlet of which had been blocked, and after fractionation, samples of bile were analyzed for bile salts by the method of Groufsky et a& (1977).…”

Section: Methodsmentioning

confidence: 99%

“…(Abernathy et alii, 1975 ;Utili et a&, 1976). Recent studies on the hypothermic perfusion of rat liver in this laboratory (Lee and Walker, 1977 ;Groufsky et a&, 1977) have employed BSA at 70 g/l. in order to raise the oncotic pressure of the perfusate to about its physiological level.…”

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confidence: 99%

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The Effect of Colloid Concentration on the Functional Characteristics of Isolated Perfused Rat Liver

Lee

Holland

Groufsky

1977

Australian and New Zealand Journal of Surgery

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The volume of bile produced by isolated perfused r a t liver was dependent on the concentration of bovine serum albumin (BSA) in t h e perfusate, the lowest volumes being obtained w i t h Krebs Ringer bicarbonate ( K R B ) alone and with 70 g/l. BSA In KRB. Increasing amounts were produced as the concentration o f BSA was reduced f r o m 70 g/l. t o 8.5 g/l. T h e concentration of bile salts in the bile decreased rapidly during the first hour, b u t much more slowly thereafter. T h e highest concentration observed during the first hour was w i t h K R B alone and the lowest w i t h 20 g/l. BSA;subsequently, t h e highest concentrations were obtained with 70 g/l. BSA and t h e lowest w i t h 20 g/l. BSA. Urea synthesis was lowest w i t h K R B alone, but increased as the concentration of BSA increased; however, values obtained w i t h 8.5, 20, and 40 g/l. were f a i r l y close together. W i t h K R B alone there was a rapid release of potassium during the f i r s t hour, but thereafter the rate of release was similar t o t h a t found when BSA was present. The lowest concentrations o f potassium were obtained w i t h 20 and 40 g/l. BSA. W i t h K R B alone, GOT was released f r o m the start of perfusion o f 300 units per minute. W i t h BSA present, GOT release was delayed 4 hours, and thereafter the rate was dependent on t h e concentration o f BSA, being lowest w i t h 20 and 40 g/l. (200 units per min), higher w i t h 8 . 5 g/l. (350 units per m i n ) and highest w i t h 70 g/l. (400 units per mln).ATTEMPTS to preserve the isolated h e r are much less successful than with the kidney. Perfusates containing high concentrations of potassium, which have proved successful with the latter organ, have been much less effective with the liver (Lambotte et Schalm et alii, 1975 ;Toledo-Pereyra and Najarian, 1974). It seems likely, therefore, that the development of methods for preservation of the isolated liver at temperatures above 0°C will come from an understanding of the effect of the composition of perfusates on the functional characteristics of the organ.Isolated rat liver has been studied extensively, and there have been marked differences in the composition of perfusates used for its support. One major source of variation has been the concentration of colloid used in conjunction with a balanced salt solution such

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Section: Discussioncontrasting

confidence: 72%

Section: Methodsmentioning

confidence: 99%