Effect of dimethyl sulfoxide in combat with gentamicin induced nephrotoxicity in rats

Abstract

Introduction: Gentamicin sulphate (GS) induces nephrotoxicity by increasing of reactive oxygen species (ROS). Objectives: The aim of this research was to assess the renoprotective effect of dimethyl sulfoxide (DMSO) as an antioxidant agent against GS-induced nephrotoxicity. Materials and Methods: Forty male rats (Sprague-Dawley) were divided equally in to five groups and were treated as follows; group 1; received normal saline and served as normal controls, group 2; received GS (100 mg/kg/d), groups 3; received GS and DMSO (0.5 mL/kg/d), groups 4; received GS and DMSO (1 mL/kg/d), groups 5; received GS and DMSO (2 mL/kg/d). After eight days of treatment, serum was prepared. Paraffin sections (3 µ thickness) were prepared from the left kidneys and stained through periodic acid-Schiff (PAS) method. Serum creatinine and urea were assessed by the kits and serum malondialdehyde (MDA) was assessed by thiobarbituric acid (TBA) test. Volume density of proximal tubules, tubular necrosis, tubular cast and lymphocyte infiltration were evaluated histopathologically. The data were analyzed by Mann-Whitney U test at P< 0.05 by SPSS 12 software. Results: Serum MDA, creatinine, urea, tubular volume density, tubular necrosis, tubular cast and lymphocyte infiltration were ameliorated significantly in groups four and five compared with group two (P<0.05). Conclusion: DMSO improved GS-induced renal toxicity significantly through prevention of lipid peroxidation and inflammation, but could not save kidney functional tests and histological changes at the same level as that of the normal group.

An Investigation into the Effects of Ubiquinone on Inflammation, Diabetic Myopathy and Endotheliopathy, and CBC Parameters in Diabetic Rats

“…Our research validated that ubiquinone supplementation substantially improved this oxidative status by reducing MDA concentration (Figure 4). The beneficial role of ubiquinone treatment was proved to reduce lipid peroxidation and serum MDA levels, according to the findings of earlier studies [27,54,57].…”

Protective effect of Crocus sativus stamens extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney

“…Plasma levels of creatinine and urea are crucial parameters of kidney function. An increase in creatinine means impaired kidney function or kidney disease (Gilbert et al 1989;Hasanvand et al 2019;Edeogu et al 2020). The present study showed that intraperitoneal injection of GEN (80 mg/kg) induced significant nephrotoxicity (p<0.05) by increasing serum creatinine, urea, sodium, and urine volume.…”