Effect of differently sized nanoparticles’ accumulation on the optical properties of<i>ex vivo</i>normal and adenomatous human colon tissue with OCT imaging and diffuse reflectance spectra

Zhang, Y Q; Wu, Gongqing; Wei, Huajiang; Guo, Zhouyi; Yang, Hongqin; He, Yonghong; Xie, Shusen; Liu, Y

doi:10.1088/1612-2011/11/8/085901

Cited by 8 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gold nanoparticles (AuNPs) are of particular interest for OCT because they have strong localized surface plasmon resonance (LSPR) for interaction with light 44 and can be tuned towards the NIR-I and NIR-II windows. [45][46] Studies show that the size and shape of AuNPs, nanospheres, [47][48] nanocages, nanoshells, [49][50][51] nanobipyramids, 40 branched AuNPs, 52 nanoprisms, 53 nanorods, [54][55][56][57][58][59] and nanodisks, 60 have high scattering efficiencies and OCT contrast when the SPR band is within the central operating wavelength of the OCT. [61][62] Silver nanoparticles (AgNPs), 63 silica-coated gold nanostars, 64 silica-coated silver nanorods (AgNRs), [65][66] and titanium oxide (TiO2) 67 also have high scattering efficiencies. For cell labeling studies nanomaterials such as quantum dots, 68 luminescent nanoparticles, 69 iron oxide nanoparticles, 70 , and AuNPs [71][72] have been employed as they produce intense signal sensitivity to detect a low number of cells with a distinct advantage over reporter genes.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

Mackiewicz

Marquart

Stoddard

et al. 2021

Preprint

View full text Add to dashboard Cite

Gold nanorods (AuNRs) hold tremendous potential to improve the diagnosis and therapeutic options across the blood-retinal barrier to treat retinal diseases. For clinical ophthalmological translation, a fundamental understanding of how their physicochemical properties such as size, shape, charge, surface chemistry, and concentration, impact their stability biological environments, mechanism and efficiency of uptake, and toxicity is a necessity. Here we interrogated the uptake efficiency, biocompatibility, and stability of two subtypes of AuNRs with different types of surface coatings and varying charges, including a commercially available set of AuNRs with a 5 nm mSiO2-polymer coating and hybrid lipid-coated AuNRs developed in-house.Confocal and bright field microscopy images showed uptake of both subtypes of AuNRs in retinal pigment epithelium (RPE), neural progenitor (NP), and baby hamster kidney (BHK) cells.Transmission electron microscopy (TEM) confirms both types of AuNRs are taken up into the cytoplasm of the cells; however, larger aggregates of AuNRs are observed with the more positive and "sticky" AuNRs with a 5 nm mSiO2-polymer coating than the slightly negative hybrid lipidcoated AuNRs. Inductively Coupled Mass Spectroscopy (ICP-MS) confirm that ~3,000 of the slightly negative hybrid lipid-coated AuNRs cells and ~5,400 of the positively charged AuNRs with a 5 nm mSiO2-polymer coating (+35 mV) are taken up into RPE and BHK cell lines. Stability studies in a variety of cellular media showed that hybrid lipid-coated AuNRs are stable and disaggregated in water, 10 mM PBS buffer pH 7, and BHK media except for NP media. In contrast, the positively charged AuNRs with a 5 nm mSiO2-zeta polymer coating aggregated in all media, indicating more interactions with each other and components of the media. Bright-field and TEM confirm the presence of large aggregates of AuNRs on the surface and within the cytoplasm.Cytotoxicity studies both subtypes of AuNRs have an 80 ± 8 % cell viability, indicating mild toxicity. The hybrid lipid-coated AuNR with the cell-penetrating peptide had the least toxicological impact with a > 92 ± 7 % cell viability. Our study highlights the importance of evaluating the impact of the physicochemical features of each new nanoparticle design on their stability in biologically relevant environments and their impact on cellular uptake and toxicity in stem cell-derived therapeutic cells. Here we also provide a simple design strategy for tuning the surface chemistry of robust hybrid lipid-coated AuNRs to enhance cellular uptake to label stem cells with minimal aggregation and toxicity.

show abstract

Section: Introductionmentioning

confidence: 99%

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

Mackiewicz

Marquart

Stoddard

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

Mackiewicz

Marquart

Stoddard

et al. 2021

Preprint

View full text Add to dashboard Cite

Gold nanorods (AuNRs) hold tremendous potential to improve the diagnosis and therapeutic options across the blood-retinal barrier to treat retinal diseases. For clinical ophthalmological translation, a fundamental understanding of how their physicochemical properties such as size, shape, charge, surface chemistry, and concentration, impact their stability biological environments, mechanism and efficiency of uptake, and toxicity is a necessity. Here we interrogated the uptake efficiency, biocompatibility, and stability of two subtypes of AuNRs with different types of surface coatings and varying charges, including a commercially available set of AuNRs with a 5 nm mSiO2-polymer coating and hybrid lipid-coated AuNRs developed in-house. Confocal and bright field microscopy images showed uptake of both subtypes of AuNRs in retinal pigment epithelium (RPE), neural progenitor (NP), and baby hamster kidney (BHK) cells. Transmission electron microscopy (TEM) confirms both types of AuNRs are taken up into the cytoplasm of the cells; however, larger aggregates of AuNRs are observed with the more positive and “sticky” AuNRs with a 5 nm mSiO2-polymer coating than the slightly negative hybrid lipid-coated AuNRs. Inductively Coupled Mass Spectroscopy (ICP-MS) confirm that ~3,000 of the slightly negative hybrid lipid-coated AuNRs cells and ~5,400 of the positively charged AuNRs with a 5 nm mSiO2-polymer coating (+35 mV) are taken up into RPE and BHK cell lines. Stability studies in a variety of cellular media showed that hybrid lipid-coated AuNRs are stable and disaggregated in water, 10 mM PBS buffer pH 7, and BHK media except for NP media. In contrast, the positively charged AuNRs with a 5 nm mSiO2-zeta polymer coating aggregated in all media, indicating more interactions with each other and components of the media. Bright-field and TEM confirm the presence of large aggregates of AuNRs on the surface and within the cytoplasm. Cytotoxicity studies both subtypes of AuNRs have an 80 ± 8 % cell viability, indicating mild toxicity. The hybrid lipid-coated AuNR with the cell-penetrating peptide had the least toxicological impact with a > 92 ± 7 % cell viability. Our study highlights the importance of evaluating the impact of the physicochemical features of each new nanoparticle design on their stability in biologically relevant environments and their impact on cellular uptake and toxicity in stem cell-derived therapeutic cells. Here we also provide a simple design strategy for tuning the surface chemistry of robust hybrid lipid-coated AuNRs to enhance cellular uptake to label stem cells with minimal aggregation and toxicity.

show abstract

“…Currently, many imaging techniques are used for the clinical diagnosis of tumors, such as a gastroscope, computed tomography (CT), ultrasound, positron emission tomography and magnetic resonance imaging (MRI) [9][10][11]. A gastroscope is a rapidly evolving imaging tool in the diagnosis and treatment of gastric cancer with the advantages of safety, minimal morbidity and mucosal sensitivities [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Electroporation-assisted discrimination of normal, benign and cancerous human gastric tissues by OCT and diffuse reflectance spectra images

Wei

Zhao

et al. 2018

Laser Phys.

View full text Add to dashboard Cite

The purpose of this study is to illustrate experimentally the optical coherence tomography (OCT) signal slope and diffuse reflectance (DR) spectra of 30% and 80% glycerol combined with electroporation (EP) diffusion in normal, benign and cancerous human gastric tissues in vitro. The results of OCT showed that the permeability coefficients of 80% and 30% glycerol (both with and without EP) have the following trend: human cancerous gastric tissue > human benign gastric tissue > human normal gastric tissue under the same conditions. The permeability coefficient of the 30% glycerol group is larger than that of the 80% glycerol group under the same circumstances; the permeability coefficient of glycerol combined with the EP group is larger than that without the EP group under the same conditions. The permeability coefficient and the reduction of the DR spectra have perfect linear correlation (R 2 = 0.9745). The research results suggest that OCT and the DR spectra combined with an optical clearing agent (glycerol) and the EP method can potentially become a powerful tool for the early diagnosis and monitoring of human gastric cancer.

show abstract

Effect of differently sized nanoparticles’ accumulation on the optical properties ofex vivonormal and adenomatous human colon tissue with OCT imaging and diffuse reflectance spectra

Cited by 8 publications

References 33 publications

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

The Impact of Surface Chemistry on Gold Nanorods Uptake in Stem Cell-derived Therapeutic Cells

Electroporation-assisted discrimination of normal, benign and cancerous human gastric tissues by OCT and diffuse reflectance spectra images

Contact Info

Product

Resources

About