Effect of Different Temperature and Culture Media on the Mycelia  Growth of Trichoderma viride Isolates

Maurya, Manoj Kumar; Srivastava, Mukesh; Singh, Anuradha; Pandey, Sonika; Ratan, Ved

doi:10.20546/ijcmas.2017.602.032

Cited by 4 publications

(5 citation statements)

References 3 publications

(2 reference statements)

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“…The number of replications for each treatment was three. All Petri dishes were divided into two groups; the first was incubated at 25 • C and the second was incubated at 35 • C. Mycelial growth was recorded after seven days [14].…”

Section: Growth Under Thermal Stress and Different Substratesmentioning

confidence: 99%

“…At 25 ºC on PDA, Ta1 and Tv8 had the highest and lowest growth rates, respectively, and on CMD, Th6 and Th4 had the highest and lowest growth rates, respectively. Several studies have claimed that potato dextrose medium is the best choice for the growth of Trichoderma fungi [6,14,15,72]. One study [73] evaluated the growth of T. harzianum on five culture media namely, PDA, modified potato dextrose agar, water agar (WA), carrot agar (CA) and cornmeal agar (CMA), PDA and WA were found to be more and less effective for growth, respectively.…”

Section: Growth Under Thermal Stress and Different Substratesmentioning

confidence: 99%

“…Inhibition of pathogens growth in the contact zone with Trichoderma spp. in dual cultures might be attributed to the production of inhibitory volatile and non-volatile compounds such as terpenes, pyrones, and polyketides [13], production of extracellular hydrolytic enzymes [7], and inactivation of the pathogen's enzymes [14].…”

Section: Ability To Antagonize Soil-borne Pathogenic Fungimentioning

confidence: 99%

“…Trichoderma spp. plays an essential role through pathogens inhibition under the contact zone due to its multiple biocontrol mechanisms such as mycoparasitism, antibiosis, competition for sites and nutrients [11], availability of metals [12], non-volatile and volatile compounds production [13], extracellular hydrolytic enzymes production [7], and pathogen's enzymes inactivation [14]. Biotic and abiotic factors influence antagonistic activity and growth of Trichoderma species against pathogens, including growth medium [6,15], temperature [16] and metal availability [17].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Hewedy

Lateif

Seleiman

et al. 2020

Biology

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Trichoderma species are known as excellent biocontrol agents against soil-borne pathogens that cause considerable crop losses. Eight strains of Trichoderma were isolated from five Egyptian regions. They identified based on translation elongation factor-1α (TEF1) sequencing as four different Trichoderma species: Trichoderma asperellum, Trichoderma harzianum, Trichoderma viride, and Trichoderma longibrachiatum. Optimal growth conditions (temperature and media), and the phosphate solubilization capability of Trichoderma strains were evaluated in vitro. Further, the ability of these strains to antagonize Fusarium solani, Macrophomina phaseolina, and Fusarium graminearum was also evaluated. The results revealed that Trichoderma harzianum (Th6) exhibited the highest antagonistic ability against F. solani, M. phaseolina and F. graminearum with inhibition rates of 71.42%, 72.97%, and 84.61%, respectively. Trichoderma viride (Tv8) exhibited the lowest antagonism against the same pathogens with inhibition rates of 50%, 64% and 69.23%, respectively. Simple-sequence repeats (SSRs) and random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic variability of the Trichoderma strains. The results revealed that of 45 RAPD amplified bands, 36 bands (80%) were polymorphic and of SSRs amplified 36 bands, 31 bands (86.11%) were polymorphic. The amplification of calmodulin and β-1,3-endoglucanase was noted at 500 bp and 230 bp, respectively. Data indicated that T. viride (Tv8) had the highest phosphate solubilization index (10.0 mm), while T. harzianum (Th6) had the lowest phosphate solubilization index (4.0 mm). In conclusion, T. harzianum (Th6) had the highest antagonistic activity in dual culture assay along with the growth rate; while T. viride (Tv8) had the highest phosphate solubilization activity. There are still gaps in obtaining new formulations, selecting potent Trichoderma strains to confirm disease control in planta. For improving Trichoderma recommendation in the organic agricultural system and sustaining the fertility of the soil, the field application of highly antagonistic biocontrol agents in different types of soil and plant species will be the first approach toward bio-pesticide treatments along with bio-fertilizer inoculation. Furthermore, secondary metabolites will be investigated for the most promising strains with the combination of different pathogens and application timing.

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Section: Growth Under Thermal Stress and Different Substratesmentioning

confidence: 99%

Section: Growth Under Thermal Stress and Different Substratesmentioning

confidence: 99%

Section: Ability To Antagonize Soil-borne Pathogenic Fungimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Hewedy

Lateif

Seleiman

et al. 2020

Biology

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“…Sharma et al [11] also reported that media, pH, and temperature showed profound effects on the growth and sporulation of fungi. Similarly, the effects of different temperatures and culture media on ten strains of Trichoderma viride were studied by Maurya et al [5] . The maximum growth of Trichoderma was found at 25-30 °C and the maximum weight was found in potato dextrose agar (276 mg), while the lowest was recorded in Czapek Apart from the effect of cultural studies on rhizospheric Trichoderma isolates, this paper describes a comparison between the cultural characteristics of rhizospheric and nonrhizospheric Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Extensive intra-species comparative assessment of nutrient media for growth between Rhizospheric and Non-Rhizospheric Trichoderma Isolates

Chaithra,

Pankaja,

Mahadeva

et al. 2024

Int. J. Adv. Biochem. Res.

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For their growth characteristics on seven distinct media, five Trichoderma isolates from nonrhizospheric regions were compared to one rhizospheric and one commercial isolate. The majority of Trichoderma isolates showed early sporulation and maximum mycelial development on corn meal agar and oat meal agar media, two of the seven different solid media. In terms of mycelia growth (90 mm) and early sporulation (48 hours after inoculation) on the greatest number of different solid media, nonrhizospheric isolates namely, PSV (Paddy straw) and GMV (Goat manure) performed better than commercial isolates. A rhizospheric Trichoderma isolate, Rkd-Cu, came in second (66 mm). Nonrhizospheric sources can also be used in search of effective bio-control agents, especially Trichoderma spp. for eco-friendly management against various plant pathogens, as fungi with the fastest growth rate and the earliest sporulation contribute to the virulence of bio-control agents against plant pathogens.

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Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases - A review

Asad

2022

Ecological Complexity

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Effect of Different Temperature and Culture Media on the Mycelia Growth of Trichoderma viride Isolates

Cited by 4 publications

References 3 publications

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Extensive intra-species comparative assessment of nutrient media for growth between Rhizospheric and Non-Rhizospheric Trichoderma Isolates

Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases - A review

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