Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro

Pan, Qunxing; Huang, Kehe; He, Kongwang; Lu, Fuzeng

doi:10.1016/j.jtemb.2008.02.002

Cited by 27 publications

(26 citation statements)

References 19 publications

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“…Selenium deficiency also has a significant impact on the morphology and influenza-induced host defense responses in human airway epithelial cells (35). Porcine circovirus type 2 replication in PK-15 cells is inhibited by DLselenomethionine in a concentration-dependent manner (277). Except for Keshan disease no causal links between selenium intake and viral response have been demonstrated in humans, but selenium status and viral responses are associated.…”

Section: Immune Systemmentioning

confidence: 99%

Selenium in Human Health and Disease

Fairweather‐Tait

Bao

Broadley

et al. 2011

Antioxidants & Redox Signaling

1,071

820

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This review covers current knowledge of selenium in the environment, dietary intakes, metabolism and status, functions in the body, thyroid hormone metabolism, antioxidant defense systems and oxidative metabolism, and the immune system. Selenium toxicity and links between deficiency and Keshan disease and Kashin-Beck disease are described. The relationships between selenium intake/status and various health outcomes, in particular gastrointestinal and prostate cancer, cardiovascular disease, diabetes, and male fertility, are reviewed, and recent developments in genetics of selenoproteins are outlined. The rationale behind current dietary reference intakes of selenium is explained, and examples of differences between countries and/or expert bodies are given. Throughout the review, gaps in knowledge and research requirements are identified. More research is needed to improve our understanding of selenium metabolism and requirements for optimal health. Functions of the majority of the selenoproteins await characterization, the mechanism of absorption has yet to be identified, measures of status need to be developed, and effects of genotype on metabolism require further investigation. The relationships between selenium intake/status and health, or risk of disease, are complex but require elucidation to inform clinical practice, to refine dietary recommendations, and to develop effective public health policies.

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Section: Immune Systemmentioning

confidence: 99%

Selenium in Human Health and Disease

Fairweather‐Tait

Bao

Broadley

et al. 2011

Antioxidants & Redox Signaling

1,071

820

View full text Add to dashboard Cite

show abstract

“…Extracted DNA from PK-15 cells, and assayed by SYBR green real-time PCR [22]. In short, PK-15 cells were pretreated with different concentrations of APS in for 12 h before PCV2 infection, or subjected to APS and PCV2 simultaneously, and then harvested the cells 60 h post-inoculation.…”

Section: Quantitative Real-time Pcr For the Quantification Of Pcv2 Dnamentioning

confidence: 99%

Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway

Xue

Gan

Zhang

et al. 2015

International Journal of Biological Macromolecules

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“…11 Briefly, the PCV2-infected PK15 cells were harvested at 72 hours post-inoculation and DNA extraction was carried out using a TaKaRa DNA Mini kit (TaKaRa Co., China). The purified DNA was used as a template for real-time PCR amplification.…”

Section: Quantification Of Pcv2 Dna By Real-time Polymerase Chain Reamentioning

confidence: 99%

“…It has been reported that the addition of antioxidant feed additives can ameliorate the clinical effects of PMWS and selenium, an antioxidant trace element, has also been shown to reduce PCV2 replication in PK15 cells in a concentration-dependent manner. 10,11 However, the mechanisms by which these antioxidants influence PCV2 infection have not been elucidated. In addition, there is no information available concerning either the effects of PCV2 infection on intracellular redox status in host cells or whether intracellular redox status affects the replication of PCV2.…”

Section: Introductionmentioning

confidence: 99%