“…Sectioning was carried out using a precision cutting machine (Isomet 5000, Buehler, Lake Bluff, IL, USA), with an ample supply of cooling water, in accordance with the International Organization of Standards (ISO standards 6872:2015) . Subsequently, the specimens underwent polishing and crystallization as mentioned in previous studies ( Ellakany et al, 2021a , Ellakany et al, 2023a , Ellakany et al, 2023b ) in the research lab of College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.…”