New emission standards implemented in EU has resulted in an increasing use of passenger hybrid vehicles as well as hybrid heavy-duty transport vehicles. Manufacturers have been offering a wide range of efficient and lower emission vehicles to fulfil these Regulations. Besides commercial vehicles, it it's also important to consider the use and implementation of hybrid urban buses because it is a massive way of transportation that is used every day in the bigger cities. The use of hybrid urban buses is significant to achieve the goal for lower emissions and better energy efficiency, due to the city utilization involves several stops and accelerations. This paper considers the comparison between the results obtained from software simulation of a complete in-series hybrid urban bus platform (develop under AVL Cruise® software) and the data obtained from real on-board measurements made in the city of Madrid. The model involves an in-series hybrid electric bus, meaning that the propulsion system is supplied by an electric motor which receives energy from a stored source (battery). The analysis of the results shows a very accuracy approach and lower emission vehicle, with an error below 5% in fuel consumption modelling a real driving cycle up to 8km.