Effect of different quality of light on growth and production of secondary metabolites in adventitious root cultivation of Hypericum perforatum

Najafabadi, Ahmad Sobhani; Khanahmadi, Morteza; Ebrahimi, Mortaza; Moradi, Kosar; Behroozi, P.; Noormohammadi, Nafiseh

doi:10.1080/15592324.2019.1640561

Cited by 40 publications

(25 citation statements)

References 34 publications

(26 reference statements)

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“…Light (light quality, photoperiod, and light intensity) is an essential environmental factor that affects most plant metabolic processes. There is evidence that light intensity and light quality affect AR development and the photosynthate quantity of Hypericum perforatum [40].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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Background Lotus is an aquatic horticultural crop that is widely cultivated in most regions of China and is used as an important off-season vegetable. The principal root of lotus is degenerated, and adventitious roots (ARs) are irreplaceable for plant growth. We found that no ARs formed under darkness and that exposure to high-intensity light significantly promoted the development of root primordia. Four differential expression libraries based on three light intensities were constructed to monitor metabolic changes, especially in indole-3-acetic acid (IAA) and sugar metabolism. Results AR formation was significantly affected by light, and high light intensity accelerated AR development. Metabolic changes during AR formation under different light intensities were evaluated using gene expression profiling by high-throughput tag-sequencing. More than 2.2 × 104 genes were obtained in each library; the expression level of most genes was between 0.01 and 100 (FPKF value). Libraries constructed from plants grown under darkness (D/CK), under 5000 lx (E/CK), and under 20,000 lx (F/CK) contained 1739, 1683, and 1462 upregulated genes and 1533, 995, and 834 downregulated genes, respectively, when compared to those in the initial state (CK). Additionally, we found that 1454 and 478 genes had altered expression in a comparison of libraries D/CK and F/CK. Gene transcription between libraries D/F ranged from a 5-fold decrease to a 5-fold increase. Twenty differentially expressed genes (DEGs) were involved in the signal transduction pathway, 28 DEGs were related to the IAA response, and 35 DEGs were involved in sugar metabolism. We observed that the IAA content was enhanced after seed germination, even in darkness; this was responsible for AR formation. We also observed that sucrose could eliminate the negative effect of 150 μMol IAA during AR development. Conclusions AR formation was regulated by IAA, even in the dark, where induction and developmental processes could also be completed. In addition, 36 genes displayed altered expression in carbohydrate metabolism and ucrose metabolism was involved in AR development (expressed stage) according to gene expression and content change characteristics.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Cheng¹,

Han²,

Minrong³

et al. 2020

BMC Genomics

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“…Irradiation with blue LEDs triggered increased phenolic compound accumulation and phenolic compound biosynthesis-related gene expression [ 59 , 74 , 77 ]. Irradiation with red LEDs alone was also shown to enhance the concentrations of important phytochemicals in various plant species [ 65 , 69 , 70 , 73 ]. Additionally, irradiation with LED combinations at different ratios enhanced phytochemical contents in plant species [ 54 , 56 , 67 , 71 , 76 , 177 , 178 , 179 ].…”

Section: Resultsmentioning

confidence: 99%

Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

et al. 2021

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Light is a key factor that affects phytochemical synthesis and accumulation in plants. Due to limitations of the environment or cultivated land, there is an urgent need to develop indoor cultivation systems to obtain higher yields with increased phytochemical concentrations using convenient light sources. Light-emitting diodes (LEDs) have several advantages, including consumption of lesser power, longer half-life, higher efficacy, and wider variation in the spectral wavelength than traditional light sources; therefore, these devices are preferred for in vitro culture and indoor plant growth. Moreover, LED irradiation of seedlings enhances plant biomass, nutrient and secondary metabolite levels, and antioxidant properties. Specifically, red and blue LED irradiation exerts strong effects on photosynthesis, stomatal functioning, phototropism, photomorphogenesis, and photosynthetic pigment levels. Additionally, ex vitro plantlet development and acclimatization can be enhanced by regulating the spectral properties of LEDs. Applying an appropriate LED spectral wavelength significantly increases antioxidant enzyme activity in plants, thereby enhancing the cell defense system and providing protection from oxidative damage. Since different plant species respond differently to lighting in the cultivation environment, it is necessary to evaluate specific wavebands before large-scale LED application for controlled in vitro plant growth. This review focuses on the most recent advances and applications of LEDs for in vitro culture organogenesis. The mechanisms underlying the production of different phytochemicals, including phenolics, flavonoids, carotenoids, anthocyanins, and antioxidant enzymes, have also been discussed.

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“…phytochromes, blue-light receptor and less explored UV-B photoreceptors ( Jiao et al., 2007 ). Studies by Najafabadi et al. (2019) , highlighted the significance of red light on the growth and hypericins (therapeutically important secondary metabolite) from in vitro culture of Hypericum perforatum .…”

Section: Resultsmentioning

confidence: 99%

Nexus between light and culture media on morphogenesis in Bacopa monnieri and saponin yield thereof

Aggarwal

Mathur

2020

Heliyon

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Bacopa monnieri, a well-documented nootropic plant of high commercial global demand had been explored for its effect in alleviating other diseases and symptoms. This is primarily attributed to different phytocompounds present in the plant. One of the major constituents among them are saponins. However, variation in agro-climatic conditions and choice of germplasm often affect the growth rate and yield of phytocompounds that significantly impact the efficacy of the plant and its extract. Tissue culture has been attempted to improve the yield of phytocompounds but is often restricted by higher cost and scalability. Current study explores the role of commercial hydroponic media 'Leafy 200' vis-a-vis Murashige and Skoog (MS) media, under different color and intensity of lights, on plant morphogenesis, biomass and saponin yield. Blue light induced more shoot differentiation than normal white light. Statistical studies performed using fractional factorial design showed no significant variations in the yield of saponins among the extracts. The study suggests that hydroponic culture to be a sustainable solution and possible substitute to tissue culture that may be exploited for scalable cultivation of the plant.

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Effect of different quality of light on growth and production of secondary metabolites in adventitious root cultivation of Hypericum perforatum

Cited by 40 publications

References 34 publications

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Gene expression profiling reveals the effects of light on adventitious root formation in lotus seedlings (Nelumbo nucifera Gaertn.)

Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

Nexus between light and culture media on morphogenesis in Bacopa monnieri and saponin yield thereof

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