Effect of Different Pollination Dates With Irradiated Pollens on Fruit Set, Haploid Embryo Induction and Plant Obtention in Turkish (Kirkagac, Yuva and Hasanbey) Melons

Sarı, Nebahat; Solmaz, I.; Kasapoglu, Serkan; Gursoy, I.; Szamosi, Csaba; Ünlü, Hüsnü; Park, K. S.

doi:10.17660/actahortic.2010.871.88

Cited by 9 publications

(5 citation statements)

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“…We used 300 Gy of Co 60 and obtained 42.79% average fruit setting. Our result is compatible with a previous work reported by Sari et al, (2010) who used 300 Gy of Co 60 and found the rate of the fruit set as 42% in Kirkagac melons and 46% in Yuva-Hasanbey melons. However, a higher rate of fruit set (71.6%.)…”

Section: Discussionsupporting

confidence: 93%

“…However, melon is known to be the most successful species of the family Cucurbitaceae in terms of irradiated pollen technique. Since the first report, several studies have been conducted (Sarı et al, 1992;Abak et al, 1996;Koksal et al, 2002;Lotfi et al, 2003;Yetisir and Sari, 2003;Lim and Earle, 2008;Lotfi and Salehi, 2008;Ari et al, 2010;Gursoy et al, 2010;Sari et al, 2010;Gonzalo et al, 2011;Solmaz et al, 2011;Baktemur et al, 2013) and so far this technique is routinely used in melon breeding programs.…”

Section: Introductionmentioning

confidence: 99%

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Effect of different irradiation sources and doses on haploid embryoinduction in Altinbas (Cucumis melo L. var. inodorus) melons

Dal¹,

Sarı²,

Solmaz³

2016

Turk J Agric For

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In vivo induction of parthenogenesis using an irradiated pollen technique provides successful results in melon and accelerates breeding by shortening the time for obtaining 100% homozygous lines. Gamma rays (cobalt-60) are usually used for the pollen irradiation, although it is difficult to find a suitable source for irradiation. Haploid technology is widely used in melon breeding programs, so there is a need to find alternative sources for pollen irradiation. This study was performed to compare the efficiency of different irradiation sources to induce haploid embryos. One dose of cobalt-60 (300 Gy), two different doses (200 Gy and 300 Gy) of cesium-137, and two different doses (200 Gy and 300 Gy) of X-ray were used for the irradiation of melon pollen. All irradiation sources were efficient for fruit set, while the highest fruit set (60.38%) was obtained from 200 Gy cesium-137 and the lowest (20.01%) was obtained from 300 Gy X-ray. A total of 2294 haploid embryos were rescued, of which 2052 regenerated into plants. The number of rescued haploid embryos per fruit ranged between 8.77 (300 Gy cobalt-60) and 4.23 (200 Gy X-ray). The highest regeneration rate of haploid embryos into plants was 92.56% (300 Gy cobalt-60) and the lowest was 61.86 % (200 Gy cesium-137).

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Effect of different irradiation sources and doses on haploid embryoinduction in Altinbas (Cucumis melo L. var. inodorus) melons

Dal¹,

Sarı²,

Solmaz³

2016

Turk J Agric For

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“…Recently, the application of this method resulted in haploid induction in C. maxima and C. moschata (Kurtar et al 2009, Kurtar andBalkaya 2010). Further attempts have also been made to optimise the method for cucumber and melon (Lofti et al 2003, Claveria et al 2005, Lofti and Salehi 2008, Ari et al 2010, Sari et al 2010, Godbole and Murthy 2012. So far pollination with irradiated pollen has been the leading method of haploid induction in these two species.…”

Section: Pollination With Irradiated Pollenmentioning

confidence: 99%

“…One of the most common methods used for the detection and isolation of embryos consists of the physical opening of all seeds , Kurtar et al 2002, Sari et al 2010, Godbole and Murthy 2012. At the Department of Plant Genetics, Breeding and Biotechnology cucumber seeds are inspected over a light source and the transparent (empty) seeds are discarded.…”

Section: Embryo Detection Excision and Culturementioning

confidence: 99%

Review of research on haploid production in cucumber and other cucurbits

Gałazka

Niemirowicz-Szczytt

2013

Folia Horticulturae

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This review provides a summary of haploid induction methods and factors affecting the efficacy of specific methodologies as applied to cucumber (Cucumis sativus L.), melon (Cucumis melo L.), watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai), winter squash (Cucurbita maxima Duch. ex Lam.), summer squash (Cucurbita pepo L.) and other cucurbits. This report is focused on studies that were carried out during the last 20 years. The main objective of the research on the production of haploid cucurbit plants is to accelerate breeding programs through the use of homozygous double haploid lines (DHL) and to facilitate the selection of desired (e.g. disease-resistant) genotypes for breeding. Unfortunately, currently used protocols result in a low number of double haploids (DH). The most common and best-known method of obtaining haploid cucurbit plants is via pollination with irradiated pollen, which induces parthenogenetic development of haploid embryos in planta. The embryos are extracted from immature seeds and cultured in vitro to facilitate the maturation and development of plants. The studies described below were primarily aimed at the determination of an appropriate dose of radiation and the evaluation of the impact of the genotype and the time of year on the number of haploid embryos and plants obtained. A less popular method of haploid production - ovule and ovary culture - is based on in vitro gynogenesis. The studies related to this method concentrated on optimising the composition of the medium and pre-treatment conditions (primarily temperature) to which the flower buds were subjected. Recently, increasing attention has been paid to anther and microspore culture. As in the case of in vitro ovule and ovary culture, the medium composition and flower bud pre-treatment conditions were optimised. The most recent studies suggest that anther culture is comparable in effectiveness to the irradiated pollen technique.

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“…In vitro androgenesis, gynogenesis and parthenogenesis are the unique methods for the recovery of fertile homozygous DH's. In Cucurbitaceous, irradiated pollen technique is the efficient method to generate the genetically uniform DH's in melon [Sari et al 2010] and cucumber [Lotfi et al 1999], but this technique has not yet been fully optimized for production of high quantity haploids in squash [Kurtar et al 2002], pumpkin [Kurtar et al 2009] and winter squash [Kurtar and Balkaya 2010]. Thus, the alternative method, we conducted to anther culture with the aim of developing high quantity DH's in winter squash and pumpkin [Kurtar et al 2016].…”

Section: Introductionmentioning

confidence: 99%

THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

Kurtar¹

2018

Acta Sci.Pol.Hortorum Cultus

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Double-haploidization (DH) is one of the favorable techniques to obtain 100% pure double haploid plants (DH's) for generating the new F 1 cultivars in a short time. The fecundity of this technique depends on the high quantity of haploids and also fertile DH's. However, there are no comprehensive reports on the chromosome doubling and fertility (fruit and seed-set) of winter squash and pumpkin haploids, currently. Thus, to obtain high frequency and fertile DH's, the efficiency of different anti-mitotic agents (colchicine, amiprophos methyl, trifluralin and oryzalin) was tested at various concentrations and exposure times for both in vitro and in vivo conditions. Haploid plantlets recovered from anther cultures of winter squash and pumpkin lines were used for DH program. The haploid plants were wholly immersed in aqueous solutions of antimitotic agents in vitro and apical parts of haploid plantlets were treated with anti-mitotic agents three times in vivo. Since some plants remained haploid, and the highest DH efficiency was obtained from multiple treatments of colchicine to shoot tips of haploid plants in the rate of 93.3%. In vivo multiple treatments of 1% colchicine for an hour was found to be the best doubling procedure for the recovery of high-frequency fertile DH's in our winter squash and pumpkin breeding program.

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Effect of Different Pollination Dates With Irradiated Pollens on Fruit Set, Haploid Embryo Induction and Plant Obtention in Turkish (Kirkagac, Yuva and Hasanbey) Melons

Cited by 9 publications

References 0 publications

Effect of different irradiation sources and doses on haploid embryoinduction in Altinbas (Cucumis melo L. var. inodorus) melons

Effect of different irradiation sources and doses on haploid embryoinduction in Altinbas (Cucumis melo L. var. inodorus) melons

Review of research on haploid production in cucumber and other cucurbits

THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

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