Effect of different planting areas on the chemical compositions and hypoglycemic and antioxidant activities of mulberry leaf extracts in Southern China

Abstract

Guangdong, Guangxi and Chongqing are emerging sericulture areas in China where the production of mulberry leaves is huge. In order to identity high quality mulberry leaves that are suitable for healthy products to expand planting, 24 samples from three regions (Guangdong, Guangxi, Chongqing) in the south of China were quantified for two alkaloids (1-deoxynojirimycin and fagomine) and five phenols (chlorogenic acid, rutin, isoquercitrin, etc.) using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Additionally, the total phenolic and total flavonoid contents, antioxidant and glycosidase inhibitory activities (hypoglycemic activity) were tested using different assays (DPPH, ABTS, FRAP) to comprehensively evaluate the quality of the mulberry leaves. The contents of DNJ and fagomine ranged from 0.401±0.003 to 5.309±0.036 mg/g and from 0.279±0.031 to 2.300±0.060 mg/g, respectively. The main phenolic constituents were chlorogenic acid, rutin and isoquercitrin, with chlorogenic acid present in the highest concentrations, ranging from 3.104±0.191 to 10.050±0.143 mg/g. The antioxidant activity exhibited a tendency as follows: Guangxi > Guangdong > Chongqing, except for two samples from Chongqing, which showed the highest antioxidant activity. Based on our study, mulberry leaves from Guangdong and Guangxi could be future sources of natural hypoglycemic and antioxidant products.

Effect of cellulase and Lactobacillus casei on ensiling characteristics, chemical composition, antioxidant activity, and digestibility of mulberry leaf silage

“…Means in the same row followed by different capital superscript letters differ (P < 0.05). investigations and those of previous studies showed that nutritional and functional components of mulberry leaves were highly dependent on their variety, planting area, and conservation method (Hao et al, 2018;Neto et al, 2018;Yu et al, 2018). Zheng et al (2017) found wide variation in the contents of DM (61.94 to 79.30%), CP (13.61 to 24.97%), and WSC (3.99 to 17.44%) of leaves from 45 mulberry germplasms and varieties.…”

Effects of Mulberry (Morus alba L.) Leaf Extracts on Growth, Immune Response, and Antioxidant Functions in Nile Tilapia (Oreochromis niloticus)

“…Pharmacological researches has shown that the extracts of mulberry leaf have a range of promising bioactivities, including immunomodulatory activity, anti-inflammatory activity, antioxidant activity, antidiabetic activity, hepatoprotective activities and antiobesity activities (El-Sayyad, 2015;Hao et al, 2018;He et al, 2018;Wang et al, 2012).…”

UPLC-DAD-QTOF/MS Analysis of Flavonoids from 12 Varieties of Korean Mulberry Fruit

“…For example, phenylalanine ammonia-lyase is reported as the key enzyme in the formation of flavonoids in mulberry leaves. e expression of the phenylalanine ammonia-lyase gene can be induced by environmental factors such as low temperatures, and content of flavonoids can be increased under such conditions [49]. However, there is a general lack of studies on the variation of flavonoid compounds in mulberry fruit caused by environmental factors, such as growing conditions, regional differences, and climatic conditions.…”