Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish (Danio rerio) spermatozoa

Abstract

An increase in reactive oxygen species (ROS) or decrease in antioxidant barriers can provoke lipid peroxidation of the membranes or DNA damage of the spermatozoa. The aim of this work is to study the effect of the different degrees of oxidative stress generated by H O incubation on total motility, kinetics, and DNA fragmentation of zebrafish (Danio rerio) spermatozoa. For this process, experimental groups were incubated in 50 µM (Low; L) and 200 µM (High; H) H O , respectively, for 20 min at 4ºC. Sperm motility parameters were obtained with a computer-assisted sperm analysis (CASA) system. Sperm DNA fragmentation (SDF) was assessed using the sperm chromatin dispersion test. Both low and high H O concentration groups showed lower motility than control groups. Progressive motility of spermatozoa incubated in the H group dropped rapidly in comparison with other groups. Regarding SDF, the control and L groups had significantly lower values than the H group (25.0% and 31.6% vs. 48.1% fragmented sperm for C, L, and H groups, respectively; p < 0.05). Sperm motility, mostly progressive motility, decreased as H O concentration increased, mainly when time after sperm activation increased. SDF increased as the H O concentration increased. However, measurements of the halo area did not agree with the subjective SDF rate.

Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model

“…Moreover, and concomitantly with total motility, progressive motility (P-MOT) was also significantly raised ( p = 0.0137) after 21 days in the PROBIO group from 15.22% ± 4.71% to 22.73% ± 5.09% (Figure 2E; Table S2). P-MOT is another key parameter in sperm quality since it influences fertilization success, and it is a focus of attention in zebrafish research [25,26]. In our experiment, we considered progressive those cell in which swimming track was forwards in 80% of a straight line.…”

Sperm Quality Assessment in Honey Bee Drones

“…The loss of redox homeostasis in sperm may generate oxidative stress that may have deleterious effects on the spermatozoa, including lipid peroxidation of the membranes and DNA damage [118]. In fact, spermatozoa are more susceptible to oxidative stress than somatic cells because they contain a reduced cytoplasm with few antioxidants and their plasma membrane is rich in unsaturated free acids highly susceptible to peroxidation.…”

“…Furthermore, the high cellular metabolism of spermatozoa required to obtain flagella movement generates free radicals. This process may be aggravated by some treatments, such as dilution and cryopreservation [118].…”

“…The direct determination of oxidative stress through the analysis of reactive oxygen species (ROS) is very challenging due to their instability, the short lives of their intermediates [114] and the fact that spermatozoa intrinsically produce ROS [118]. The measurement of the defence capacity of sperm against oxidative damage and of the consequences of oxidative stress (lipoperoxidation of membranes and DNA damage) seem to be better alternatives.…”

A multiplier peroxiporin signal transduction pathway powers piscine spermatozoa

“…Such changes have logically been associated with increased mitochondrial production of ATP for improved flagellar motility. However, the biochemical reactions that lead to increased ATP synthesis also generate elevated levels of hydrogen peroxide (H 2 O 2 ), a reactive oxygen species (ROS) that inhibits mitochondrial function and suppresses flagellar motility (23)(24)(25)(26)(27). With osmotic stress of the exposed ejaculate generating additional ROS (26,28), it has been unclear how sperm evolved molecular mechanisms that surmount such signaling conflicts.…”