Effect of Different Materials used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium

Aizawa, Peter; Karlsson, Göran; Benemar, Charlotte; Segl, Vera; Martinelle, Kristina; Lindner, Elisabeth

doi:10.1007/978-1-4020-5476-1_77

Cited by 1 publication

(2 citation statements)

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“…The CM cell viability result was comparable with that of the negative control, which indicates that both the fluidic system and SIS-based vascular graft are capable to sustain viable and healthy cells and, therefore, it could be potentially used as a model to study the endothelization processes on vascular grafts. Despite some authors suggestong that inert materials such as glass and stainless steel 316 might reduce cell viability [60], our results demonstrated the opposite. This non-cytotoxic response can be attributed to the presence of 2% FBS on the recirculated medium, which contains proteins such as albumin that deposit on the surface and can potentially reduce the inherent toxicity effects of inert materials [61,62].…”

Section: Discussioncontrasting

confidence: 99%

“…This non-cytotoxic response can be attributed to the presence of 2% FBS on the recirculated medium, which contains proteins such as albumin that deposit on the surface and can potentially reduce the inherent toxicity effects of inert materials [61,62]. In addition, our results agree well with the reduced cytotoxicity of silicone, which was the material of O'rings in the fluidic system [60].…”

Section: Discussionsupporting

confidence: 80%

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Design and Characterization of a Fluidic Device for the Evaluation of SIS-Based Vascular Grafts

et al. 2020

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Currently available small diameter vascular conduits present several long-term limitations, which has prevented their full clinical implementation. Commercially available vascular grafts show no regenerative capabilities and eventually require surgical replacement; therefore, it is of great interest to develop alternative regenerative vascular grafts (RVG). Decellularized Small Intestinal Submucosa (SIS) is an attractive material for RVG, however, the evaluation of the performance of these grafts is challenging due to the absence of devices that mimic the conditions found in vivo. Thereby, the objective of this study is to design, manufacture and validate in silico and in vitro, a novel fluidic system for the evaluation of human umbilical vein endothelial cells (HUVECs) proliferation on SIS-based RVG under dynamical conditions. Our perfusion and rotational fluidic system was designed in Autodesk Inventor 2018. In silico Computational Fluid Dynamics (CFD) validation of the system was carried out using Ansys Fluent software from ANSYS, Inc for dynamical conditions of a pulsatile pressure function measured experimentally over a rigid wall model. Mechanical and biological parameters such as flow regime, pressure gradient, wall shear stress (WSS), sterility and indirect cell viability (MTT assay) were also evaluated. Cell adhesion was confirmed by SEM imaging. The fluid flow regime within the system remains laminar. The system maintained sterility and showed low cytotoxicity levels. HUVECs were successfully cultured on SIS-based RVG under both perfusion and rotation conditions. In silico analysis agreed well with our experimental and theoretical results, and with recent in vitro and in vivo reports for WSS. The system presented is a tool for evaluating RVG and represents an alternative to develop new methods and protocols for a more comprehensive study of regenerative cardiovascular devices.

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Section: Discussioncontrasting

confidence: 99%