Effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling

Horák, Daniel; Babič, Michal; Jendelová, Pavla; Herynek, Vı́t; Trchová, Miroslava; Likavčanová, K.; Kapcalová, Miroslava; Hájek, Milan; Syková, Eva

doi:10.1016/j.jmmm.2009.02.082

Cited by 54 publications

(46 citation statements)

References 35 publications

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“…In our previous study, we showed that the efficiency of cell labeling with poly-L-lysinecoated SPION ranges from 72%-84%. 35 After we seeded labeled cells on a Petri dish, they were attracted to the region above the magnet and concentrated in the target area.…”

Section: Discussionmentioning

confidence: 99%

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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Abstract:The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells' accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.

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Section: Discussionmentioning

confidence: 99%

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček¹,

Zablotskii²,

Forostyak³

et al. 2012

IJN

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“…As a result, many investigators have turned to noninvasive i maging modalities which are capable of monitoring and tracking stem cells within a single model over time. [9][10][11] The imaging approaches previously investigated require use of contrast agents to label the cells. Efficient cell labeling with contrast agents is necessary in order to image and track MSCs adequately.…”

Section: Introductionmentioning

confidence: 99%

Function of mesenchymal stem cells following loading of gold nanotracers

Ricles¹,

Nam²,

Sokolov

et al. 2011

IJN

117

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Background: Stem cells can differentiate into multiple cell types, and therefore can be used for cellular therapies, including tissue repair. However, the participation of stem cells in tissue repair and neovascularization is not well understood. Therefore, implementing a noninvasive, long-term imaging technique to track stem cells in vivo is needed to obtain a better understanding of the wound healing response. Generally, we are interested in developing an imaging approach to track mesenchymal stem cells (MSCs) in vivo after delivery via a polyethylene glycol modified fibrin matrix (PEGylated fibrin matrix) using MSCs loaded with gold nanoparticles as nanotracers. The objective of the current study was to assess the effects of loading MSCs with gold nanoparticles on cellular function. Methods: In this study, we utilized various gold nanoparticle formulations by varying size and surface coatings and assessed the efficiency of cell labeling using darkfield microscopy. We hypothesized that loading cells with gold nanotracers would not significantly alter cell function due to the inert and biocompatible characteristics of gold. The effect of nanoparticle loading on cell viability and cytotoxicity was analyzed using a LIVE/DEAD stain and an MTT assay. The ability of MSCs to differentiate into adipocytes and osteocytes after nanoparticle loading was also examined. In addition, nanoparticle loading and retention over time was assessed using inductively coupled plasma mass spectrometry (ICP-MS). Conclusion: Our results demonstrate that loading MSCs with gold nanotracers does not alter cell function and, based on the ICP-MS results, long-term imaging and tracking of MSCs is feasible. These findings strengthen the possibility of imaging MSCs in vivo, such as with optical or photoacoustic imaging, to understand better the participation and role of MSCs in neovascularization.

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“…18,23,24 CZF were shown to have good electromagnetic performance and excellent chemical stability so they are promising candidates for biomedical application. 25 However, their biological effect has not been studied in much detail.…”

mentioning

confidence: 99%

“…Similar labeling efficiency was found in PLL-coated γ-Fe 2 O 3 -labeled hMSCs and rMSCs. 23,24 Comparable labeling efficiencies were shown in the labeling of neural stem cells with various types of contrast agents. [28][29][30][31] Further, we confirmed the internalization of both types of contrast agents in cell cytoplasm using TEM.…”

mentioning

confidence: 99%

“…Generally, the relaxivities of labeled iPSC-NPs were lower than in labeled rMSCs or hMSCs, regardless of the type of nanoparticles. 22,23 While the percentage of labeled cells is comparable in MSCs and iPSC-NPs, MSCs tend to endocytose more nanoparticles, and the labeling is more intense (Turnovcova, unpublished data, 2014) than in neural precursor cells, using the same concentrations and the same labeling time. These results correspond to the significantly higher amount of iron per cell in magnetically labeled MSCs.…”

mentioning

confidence: 99%

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The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

Jiráková

Šeneklová

Jirák

et al. 2016

IJN

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Effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling

Cited by 54 publications

References 35 publications

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Function of mesenchymal stem cells following loading of gold nanotracers

The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

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